质粒

The adenovirus plasmid was identified by PacI digestion and transfected into 293A cells to package a recombinant adenovirus which expressed the Fhit protein. Furthermore, the adenovirus rAd-Fhit was infected into colon cancer cells,and the expression of the ectogenic protein was detected by Western blotting. Finally, the proliferation of colon cancer cells was observed in adenovirus-infected cells by the MTT assay.

经PacI酶切鉴定正确后，将重组腺病毒质粒转染293A细胞获得表达Fhit蛋白的重组腺病毒rAd-Fhit，将获得的重组腺病毒感染结肠癌细胞，采用蛋白印迹法检测外源Fhit蛋白的表达，并进一步观察其对细胞增殖能力的影响。

Pocine interferon α mature protein genes were amplified by reverse transcription polymerase chain reaction, and the recombinant replication-defective human adenovius serotype 5 plasmid pAd-poIFN-α was constructed. When the recombinant plasmid pAd-poIFN-α was linearized with PacI, and then transferred into HEK-293A cells, the virus plaque was isolated and purified in HEK-293A cells by three of plaque purification passages.

本研究利用RT-PCR方法扩增猪α干扰素成熟蛋白基因后构建了重组腺病毒质粒pAd-poIFN-α，经PacI酶切后转染HEK-293A细胞，3次噬斑纯化后获得了重组腺病毒rAd-poIFN-α。

The pALTER plasmid was successfully transfected into CHO cells and positive clones were screened and detected immunohistologically.

结果 含有人CD59两种突变体的重组pLATER质粒经酶切和琼脂糖凝胶电泳鉴定，得到长496 bp的电泳条带，与所插入片段完全相符。

Objective To investigate the effect of gene transfection of FKBP12.6 on cardiac function and pathological changes of canine heart failure induced by rapid ventricular pacing.

目的探讨白蛋白微泡介导的pcDNA3.1-FKBP12.6质粒转染对心衰犬心功能及心肌病理变化的影响。

With genomic DNA of Clostridium stercorarium as a template, a structural gene pel9A encoding pectate lyase was obtained by PCR amplification. The pel9A was cloned into pET28a to recombine an express plasmid pPel9A, and then the plasmid pPel9A was transformed into E. coli BL-21, expressed under the control of T7lac promoter at 37℃.

利用PCR技术从耐热梭状芽孢杆菌中扩增得到产耐热果胶裂解酶的结构基因pel9A，并将其克隆于表达载体pET28a中，最后将此重组质粒转化到受体菌E.coli BL-21中进行表达。

The key to this riddle appeal to be plasmids, which can block phage adsorption.

这个难题的关键是质粒，它能够阻碍噬菌体的吸附作用。

Locations of transgenes in the recovered plasmids were determined by southern blotting. And then adjacent sequences of transgenes integration sites in three recovered aberrant classes were subcloned and analyzed. Transgene of A-2 was inserted into common carp DNA sequences homologous to the mouse phosphoglycerate kinase-1 gene and those of A-3 and A-6 were inserted into common carp DNA sequences homologous to the human epidermal keratin 14 gene and sequence of common carp β-actin gene, respectively.

通过Southern杂交对转植基因在回收质粒上的位置进行了定位分析，并对三种回收到的变异型转植基因旁侧顺序进行了亚克隆和顺序测定。A-2的转植基因整合到了与小鼠磷酸甘油酸盐激酶-1基因同源的鲤鱼基因组顺序旁侧，A-3的转植基因整合到了与人表皮角蛋白14基因同源的鲤鱼基因组顺序旁侧，A-6的转植基因整合到了普通鲤鱼β-actin基因顺序中。

The function of proliferation and phosphorylation in vitro or in vivo was further studied.

利用基因重组的方法构建该基因的表达质粒，并研究该基因在细胞水平和活体水平对细胞增殖度磷酸化功能的影响。

This reporter plasmid provides a useful tool to investigate the hrp regulation and to dissect the pathogenesis of this phytopathogen.

该报告质粒的构建，为研究hrp基因表达调控机制，揭示植物病原细菌的致病机制提供了有效的材料。

In this paper the thymus DNA of calf and the pBs plasmid are the main objects of our studies in ultraweak luminescence. By means of PIAS(Photon-counting image acquisition), ultraweak luminescence detector and Lambda Bio 40 of UV/VIS spectrophotometer, we studied the UL of DNA induced by the three oxygen free radicals: singlet oxygen Oa, hydroxy radical and superoxide anion(O2 ). We found that the OH can greatly increase the UL intensity of DNA.

本论文以小牛胸腺DNA和质粒pBs为实验材料，利用光子图像探测系统(Photon-counting image acquistion，PIAS)、微弱发光测量仪和UV／VIS分光光度计和生物统计学的成组比较法原理对单线态氧(~1O_2)、羟自由基、超氧阴离子(O_2~-)三种氧自由基诱导DNA超弱发光的变化过程进行了研究，结果发现这三种氧自由基中·OH能诱导DNA的超弱发光发生显著的增强变化，而其他两种氧自由基对DNA的超弱发光并无显著诱导作用。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！