质粒

BALB/c mice were co-inoculated muscularly by this plasmid and the nucleic acid vaccine plasmid pVAXGE expressing the HIV-1 (Human immunodeficiency virus type I) gag-gp120 protein. The level of serum antibodies after immunization showed that the specific antibodies against HIV-1 appeared at the second week and raised to the peak level at the sixth week for the co-immunization group.

将它与表达I型人免疫缺陷病毒(Human immunodeficiency virus 1， HIV-1) gag-gp120的核酸疫苗质粒pVAXGE共同肌肉注射BALB/c小鼠，免疫3次后，以ELISA法检测免疫小鼠血清中抗HIV-1抗体水平，结果显示联合免疫组小鼠在免疫2周后已有抗体产生，6周后进入高峰。

Study on mechanism of repairing myocardial necrosis by skeletal myoblast transplantation ［J］.

血管内皮细胞生长因子真核表达质粒的构建及其在心肌细胞中的表达［J］。

HDAC inhibitor NaBu also promotes the expression of ALAS2 gene promoter reporter gene in a dose-dependent manner.

以启动子报告基因质粒转染细胞，然后用含有NaBu的培养基培养细胞，结果表明NaBu能以剂量依赖的方式增强ALAS2基因启动子转录活性。

These constructs were delivered into tobacco Nicotiana tabacum L.

通过基因枪法将质粒载体导入烟草（Nicotianatabacum L。

An F plasmid may integrate into the nucleoid of the recipient strain.

F质粒可整合入受体菌的拟核。

Nucleonics employs an expressed interfering RNA approach whereby scientists insert plasmid DNA coding for relevant short hairpin RNAs into targeted cells, inducing the cells to produce and deliver specific shRNA sequences.

nucleonics公司表示，分子干扰核糖核酸方法方法，科学家插入质粒编码有关短发夹核糖核酸植入目标细胞中，让细胞产生并传递特定的shrna序列。

Objective To construct a recombinant plasmid for co-expression of nucleoprotein and glycoprotein of rabies virus aG strain in CHO-K1 cells.

目的 构建狂犬病毒aG株核蛋白和糖蛋白双表达重组质粒，在中国仓鼠卵巢细胞(CHO-K1)中表达核蛋白和糖蛋白。

Null vector transfection as the control.

以空质粒转染组为对照。

The results showed that DNA vaccine pcDNA3.1-3-1E could remarkably increase the number of CD8+ T lymphocyte after two immunizations,and also could provide partial immune protection against homologous Eimeria challenge,such as significantly decreasing oocyst shedding,reducing the average lesion score in duodenum,improving body weight gain and so on.

结果显示，与载体对照组相比，质粒pcDNA3.1-3-1E 2次免疫后可显著提高脾CD8+ T淋巴细胞亚型数量，并具有一定的抗球虫免疫保护作用，可显著减少每克粪便的卵囊排出量，降低十二指肠病变记分，减少增重下降等。

Methods The total RNA was extracted from the brain of human embryo. The segment of open read frame of DOC-1 was amplified by RT-PCR and was further cloned into the vector, pMD18-T, and the prokaryotic expression vector, pGEX-4T-1, by turns, to produce the new construct pGEX-4T-1-DOC-1. After restriction enzymes digestion analysis and sequenced, pGEX-4T-1-DOC-1 was transformed into E.coli BL21（DE3）. GST-p12 recombinant protein was expressed under IPTG induction and purified by affinity column.

从人胎脑组织中提取总RNA，经逆转录-聚合酶链式反应扩增DOC-1的CDS序列，再通过基因重组技术将该基因片段依次克隆到pMD18-T和pGEX-4T-1载体中，构建融合表达载体pGEX-4T-1-DOC-1，经酶切、测序鉴定后，用该重组质粒转化E.coliBL21，用IPTG诱导表达，Glutathione Sepharose 4B柱亲和层析纯化重组蛋白。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！