质粒

The number of His-EGFP-CRP detection peaks increased after the protein was incubated with lysate of THP-1 cells.

为解决这一技术难题，构建了带his纯化标签和EGFP荧光标记的人CRP原核表达质粒［4］。

The cell lysate was used for Western blotting to examine the expression of the target protein.

结果 PCR、酶切和DNA测序结果均表明重组质粒pcDNA3-flag/HPC2构建正确，并可在HEK293细胞内高效表达。

Objective to explore the action of main reagent alkaline lysis method of extracting plasmid DNA.

目的 对碱裂解法提取质粒DNA中主要试剂的作用进行研究。

Finally,alkaline lysis and anion exchange chromatography were used to release and purify plasmids.

研究了包括菌体生长和质粒含量等的关系。

The Cre gene was cloned from lysogen BM25.8 by PCR. The Cre gene was then inserted in between the CaMV35S-35S double promtor and Nos terminator of PBI525 plasmid after sequencing. The expression box was cut down and inserted into plasmid pBINPLUS to obtain the final fertility-restoring expression vector pBINPLUSCre.

PCR方法从溶原菌BM25.8基因组DNA扩增出Cre基因，克隆测序验证无误后插入PBI525 CaMV35S-35S双启动子和Nos终止子之间，再将表达盒切下插入pBINPLUS质粒相应位点，得到恢复基因植物表达载体pBINPLUSCre。

Objective To determine the invasive ability of recombinant attenuated Salmonella typhimurium X4064（pQEM1）strain containing gene fragment of Plasmodium falciparium merozoite surface protein 1 （MSP1）,the stability of its plasmid, and its re-expression.

目的 检测含恶性疟原虫裂殖子表面蛋白1基因片断的重组减毒鼠伤寒杆菌X4064(pQEM1)的入侵宿主能力，所含质粒的稳定性和再表达能力。

Twenty clones showed a high level of tetracycline resistance (over 200 microgram tetracycline per ml).

测定了这些含有重组质粒的菌株抗四环素能力，其中有4株在平板上抗四环素水平超过225微克/毫升。

It was demonstrated that the BADHgene promoter region and its two mutants are active in monocot rice calli.

实验表明，质粒pDMC202中GUS基因在BADH基因启动区以及两个启动区突变体的控制下获得了表达。

The study in this paper, a Pollen-specific Lysine-rich Protein cDNA, from tomato by RT-PCR was obtained. A plant expression plasmid pBIUB-TSB was constructed and lysgene was under the control of maize ubiquitin promoter which is the highest efficient monocotyledon promoter.

本实验通过RT-PCR从番茄花粉中获得高效特异表达的高赖氨酸基因TSB的cDNA序列，构建其受控于单子叶植物Ubil强启动子下的高效表达质粒pBIUB-TSB。

Part Ⅱ Objective: To study the immunoprotective and the immunotherapic effects of the new nucleic acid vaccine of the hepatitis B: pVAX1-C3d-S2S; respectively in normal and HBV transgenic mice after being inoculated intra- muscularly with this Eukaryotic Expression Plasmid, such as the in situ production of the expressed protein in muscle tissue section and specific humoral immune response in normal mice、the variation of specific antigen、antibody and HBV DNA, especialy the changes of specific antigen in liver.

第二部分新型乙肝核酸疫苗pVAX1 C3d-S〓S免疫预防效应和治疗作用的研究目的：研究含有新型分子佐剂补体C3d的HBV adw血清型的乙肝核酸疫苗重组真核表达质粒pVAX1-C3d-S〓S接种正常BALB／c小鼠和HBV转基因小鼠后，其免疫预防作用（接种局部肌肉中特异性抗原蛋白的表达和所诱生的体液免疫应答规律）以及免疫治疗作用（血清中特异性抗原的表达、特异性抗体的产生、HBV DNA的消长和肝组织中抗原表达的变化）。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！