质粒

Altogether, these data suggest that recombinant plasmid BCSP31/pVAX1 is a good immunogen for the production of humoral and cell-mediated immune responses in mice and would be a better candidate for use in the future studies of vaccination against brucellosis.

研制的BCSP31/pVAX1重组表达质粒免疫BALB/c小鼠能产生高水平的特异性体液免疫和细胞免疫，以细胞免疫为主，并产生有效的免疫保护效果，为布氏杆菌病新型疫苗的研究奠定了基础。

The promoter activity of SV40 enhancer in the reporter vector of pCAT-Enhancer was induced by cycloheximide, a common protein synthesis inhibiter.

在蛋白质合成抑制剂放线菌酮诱导下，pCAT-E质粒中SV40增强子能启动CAT基因的表达。

The occurance rate of meningitis was related to amount of inoculum.

肺炎链球菌绿色荧光蛋白报告质粒的构建及评价。

Viral vectors capitalize on the motive of viruses to enter cells and insert their genetic material into the infected cells.

但其最高表达量却低于运动训练后质粒注射。

It is impossible to obtain cells, however, in which the target gene was persistently inhibited with siRNA, Therefore it is potential to use plasmid-based RNA interference technology to study gene function.

但是由于siRNA的不稳定性，采用siRNA研究基因功能不能得到抑制靶基因表达的稳定细胞克隆，因此以质粒为基础的RNA干扰技术表现出了它的应用潜力。

The plasmids were intradermally injected with a gene gun through 3 bullets, 2μg of plasmids and 4.5Mpa for each shoot.

于质粒注入后24~144h，检测小鼠体内的荧光素酶活性。

SDS-PAGE showed that the recombinant plasmid IPSE/pET32a expressed a fusion protein with an M r 35 700 after being induced by IPTG.

经IPTG诱导，SDS-PAGE分析，含重组质粒的转化子细菌能表达相对分子质量约35 700的蛋白分子，与预计Trx-IPSE融合蛋白的相对分子质量大小相符。

Methods The IPSE gene of S. man soni was synthesized by overlapping PCR, and confirmed by DNA sequencing. The recombinant plasmid IPSE-pET32a was constructed by inserting the gene of IPSE into expression vector pET32a at the downstream of thioredoxin coding sequence.

应用重叠PCR方法合成曼氏血吸虫IPSE基因，将其定向克隆至表达载体pET32a的硫氧还蛋白序列下游，构建重组表达质粒IPSE/pET32a。

Objective To explore the mechanisms of resistance plasmid in Klebsiella pneumoniae.

目的 探讨耐药性质粒在肺炎克雷伯杆菌中的耐药机制。

Furthermore, agonist induced internalization of DOR, but KOR failed to be internalized after agonist stimulation.

而在共转GRK2和GRK5质粒的细胞中，在U69593或Dynorphin A的刺激后，未观察到KOR的磷酸化。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！