质粒

The expression of Foxp3 was determined with immune fluoresce nt microscopy.The function of Foxp3 gene transferred...

结论以质粒PMFOXP3-IRES2-EGFP转染的CD4+CD25-T细胞对同种T细胞增殖反应具有显著抑制作用，与CD4+CD25+T细胞的抑制作用等同。

Results:The results showed that all of four mdr1-shRNA expression vector can significantly knockdown the expression of p-glycoprotein as compared with control vector,moreover,the vector targeting 508-526 sites of MDR1 gene is the best one.

结果：与对照载体相比，构建的4个MDR1-shRNA质粒载体均能有效下调MDR1的表达，P-糖蛋白的功能也明显降低，其中508-526靶位点的shRNA作用效果最好。

PART FOUR EFFECTS OF MIL-4RA EXPRESSION VECTOR ON ASTHMATIC AIRWAY INFLAMMATION AND TH1/TH2 CELL DISFUNCTION THROUGH INTRAPERITONEAL ADMINISTRATION In the study of part three, we treated the mouse asthmatic model by intratracheal administration with mIL-4RA expression plasmid and observed obvious therapeutic effects. But intratracheal administration belongs to one of the insult therapies.

第四部分：腹腔注射mIL-4RA重组载体对哮喘小鼠气道炎症及Th失衡的干预作用前一部分研究中我们通过气道局部干预途径，即气管内直接滴注的方式，观察到mIL-4RA真核表达质粒对哮喘小鼠具有很好的治疗作用，但气管内滴注毕竟属有创性治疗，存在需要实验条件高，有较大的风险性，不易反复进行等缺点，这势必大大限制其在动物实验及进一步临床研究中的实际应用价值。

It is the fact that three methionines distributed in the Hsp16.3 will begradually oxidated, in vitro, by hydrogen peroxide(H2O2). When the hsp16. 3 gene was cloned with pMV261 plasmid vector and transfected into Mycobacterium smegmatis, we found that the ability of anti-H2O2 will be decreased greatly among the strains of M. smeg. containing pMV261-hspl6. 3 while compared with the control strains of M. smeg. containing free plasmid vector. But we found that the ability of anti-H2O2 will be increased greatly among the strains of M. smeg. containing pMV261-38kDa while compared with the same control.

尽管Hsp16.3中有三个甲硫氨酸在体外能被过氧化氢(H_2O_2)逐一氧化，我们将Hsp16.3基因克隆到pMV261质粒上，并转染到耻垢分枝杆菌中发现，转染了pMV261-16.3的耻垢分枝杆菌抗过氧化氢的能力大大下降，而对照含pMV261-38kDa的耻垢分枝杆菌抗过氧化氢的能力却有明显的增强，据此，我们认为，Hsp16.3在细胞内可能不具有抗过氧化氢的能力。

The results showed that the both two plasmids could stablely maintained in 7653R under both free-living and symbiotic conditions and the stability was up to 100%.

结果表明：在自生与共生条件下，供试标记质粒在华癸中生根瘤菌中均能稳定存在，且稳定性达到100％。

Agrobacterium method was used to transform the plasmid into Gerbera hybrida.

用农杆菌介导的方法将该质粒转化到非洲菊中。

Inthe group injected with plasmid in 10% glyceryl,increase of GH was significantly deferentfrom control in 5d after injection.

其中在甘油组(质粒注射液中含10％的甘油)注射后第5天与对照组经t检验分析相差显著(P＜0.05)。

The plasmid of pMD-T-GO and pPIC3.5K were extracted from JM109 strain and then glycolate oxidase gene fragment was cloned into the expression vector (pPIC3.5K). The recombinant plasmids were transformed into E.coli TOP 10Fˊ. The recombinant clones were identified by PCR, digested with SnaB I /Not I and then sequenced.

本文首先从大肠杆菌JM109中提取质粒pMD-T-GO和pPIC3.5K，将菠菜乙醇酸氧化酶基因c DNA片段克隆至表达载体pPIC3.5K，转化进E.coli TOP 10Fˊ，利用设计的引物进行PCR扩增、SnaB I和Not I酶切鉴定，最后进行序列分析。

The appearance of MUC2protein,an intestinal goblet cell marker,was determined as formation of intestinalphenotype.

将pcDNA3.1 /Cdx2质粒和Sox2siRNAs分别及共同转染到人胃上皮细胞系GES-1中分别使Cdx2上调和Sox2下调。

Objective To amplify the hexokinase gene of Plasmodium falciparum isolate FCC1/HN, compare the sequence with that of other P. falciparum isolates and human, and construct its prokaryotic and eukaryotic expression plasmids.

目的 扩增恶性疟原虫FCC1／HN株的己糖激酶编码基因，构建其原核和真核表达重组质粒，测定其序列，比较它及它推导的蛋白质与恶性疟原虫其他株和人之间的差异。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！