质粒

The results were testified by enzymolysis and sequence scan.

酶切和测序结果均显示这两种重组质粒构建成功。

RESULTS:①Enzymolysis and nucleotide sequence analysis of recombinant plasmids showed that two VEGF-C siRNA eukaryotic expression vectors were constructed successfully.

结果：①重组质粒的酶切、核苷酸序列分析结果：经酶切和测序鉴定证实成功构建了血管内皮生长因子C小干扰RNA表达载体，建立了稳定转染细胞株。

The novel primers were evaluated by detecting BTV serotypes 1, 3, 5, 8, 10, 11, 21 and 22. The specificity of the primers was estimated by comparing to gene sequences of viruses published in GenBank, and further assessed by detecting BTV serotype 1-12 and Epizootic hemorrhagic disease virus serotype 1-4. The sensitivity and repeatability of PCR with the novel primers were evaluated by successfully detecting the recombinant plasmid pGEM-T121 containing the diagnosed nucleotide sequence.

Blast软件分析引物与GenBank发表的病毒基因的同源性，显示引物特异性较好；利用8个不同血清型BTV标准毒株进行RT-PCR检测，证实该引物能有效检测不同血清型BTV；通过BTV1-12和EHDV1-4进行检测，证实该引物具有较好的检测特异性；通过制备的含特检序列的质粒标准品P121进行检测，显示该引物检测的敏感性为105 copies，重复检测CV%为0；对模拟血清样品进行检测，结果均为阳性。

The vitro methods include autoagglutination test,calcium dependency test,pyrazinamidase test,salicin fermentation,esculin hydrolysis,Congo red colony test and virulence plasmid.

体外法包括自凝试验、依钙试验、吡嗪酰胺酶试验、水杨苷发酵、七叶苷水解、刚果红菌落试验和毒力质粒。

The construction of the recombinant plasmid carrying shRNA to HPV6bL1 lays the basis for the study of its inhibitive effect on cauliflower excrescence.

经测序，测序结果均与目的序列相同，均符合设计要求。结论 HPV6bL1 shRNA质粒重组体的成功构建为研究尖锐湿疣基因靶向抗体打下基础。

Three RNAi target sites (named as Abil1, Abil2, Abil3) targeting the E3B1 (NCBI: NM-024397), an identified target sites and positive control GAPDH-A were selected. The pGenesil-1 eukaryotic expression vectors with the neoR mark and GFP green fluorescent mark were selected to construct E3B1 RNAi plasmid. The effect of RNAi targeting various sites on E3B1 genes expression was evaluated by testing the transfection efficiency with fluorescence microscope and western blot. The most effective siRNA in inhibiting E3B1 genes were screened. The most effective siRNA in inhibiting E3B1 genes screened and used to transfect the neuronal cells. After that, the inhibitor of axon growth was added and the axon growth was observed. Result: The siRNA expressing plasmids targeting the E3B1 were constructed successfully.

选择针对E3B1(NCBI： NM-024397)RNAi的3个靶位点Abil1、Abil2、Abil3和1个不针对任何mRNA的RNAi靶位点以及甘油醛－3-磷酸脱氢酶，用带有neoR选择标志和GFP绿色荧光标志的真核表达载体pGenesil-1构建E3B1 RNAi质粒，分别转染培养的神经元，在荧光显微镜下观测转染率，经G418筛选得到单一的转染细胞，并用Western blot法检测各转染组神经元E3B1蛋白的表达情况，选出具有最佳抑制效应的siRNA转染神经元，加入轴突生长抑制物，观测轴突生长情况。

And the expressional efficiency was analyzed with flow cytometry.

并通过流式细胞仪，与同样是CMV启动转录egfp的pGFPN1质粒在细胞中的表达的水平进行了比较。

We describe the specific silencing of reporter gene lacZ in FHM cells (muscle cells of fathead minnow, a fish cell line) by either expressing small hairpin RNAs from plasmids or transfecting small interfering RNAs transcribed in itro.

在FHM细胞内通过转染质粒表达小发夹RNA或者直接转染小干扰RNA能特异沉默报告基因LacZ的表达，且siRNAs 抑制效果更加明显；这两种类型的小双链RNA在Hela细胞内同样能抑制报告基因的表达。

Then the flic gene was cloned into the E.coli expression vector pGEX-KG, which was induced with IPTG and the fusion protein was analyzed by SDS-PAGE and Western-blot.

构建重组表达质粒pGEX-flic，转化大肠杆菌工程菌BL21，经IPTG诱导表达后，通过SDS-PAGE及Western-blo对表达蛋白进行鉴定。

The study cloned the flic gene of App and performed sequencing and analysis of biological information. Polymerase chain reaction PCR was used to amplify the flic gene from.

构建猪传染性胸膜肺炎放线杆菌(Actionobacillus pleuropneumoniae， App)鞭毛蛋白编码基因的重组表达质粒，对其编码蛋白进行生物信息学分析，并将其在原核细胞中进行表达。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！