质粒

Western-blot and immunohistochemical assay methods were employed to detect the expression of FHIT. Apoptosis and cell cycle of cell were analyzed by FCMS. Morphological changes were observed by compound and electron microscope.

然后用流式细胞仪，普通光镜及透射电镜对获得稳定FHIT蛋白表达的转染组细胞和空真核表达质粒转染组细胞及未转染的亲本细胞凋亡的状况进行了考察，另外还通过流式细胞仪检测了三组浙江大学硕士学位论文细胞生长周期的惰况。

Finally we observed the condensation between the polymer and plasmid DNA by the methods of agarose electrophoresis, fluorescence spectrometer and Multi-angle laser light scattering.

最后，利用琼脂糖凝胶电泳、荧光分光光度计以及多角度激光散射手段，对合成的多聚物与质粒DNA的缩合作用进行了检测研究。

To solve this problem, we consider that use of tissue-specific regulative elements in construction of plasmid vector for molecular conjugate vector may limit the expression of foreign gene to targeting cells further.

为解决这一问题，我们考虑在构建分子偶联载体中的重组质粒表达载体时，应用组织细胞特异性调控序列，以期在转录水平进一步限制外源基因的表达于靶细胞中，从而保证受体介导基因转移的靶向性，同时可望导致外源基因的高效表达。

Objective To study the conjugational transfer and antibiotic resistance expression of pRST98 in different enteric bacilli(including E.coli, S.typhi, S.typhimurium and S.flexneri).

目的 研究伤寒杆菌耐药质粒pRST98在不同种属肠道杆菌间(大肠杆菌、伤寒杆菌、鼠伤寒杆菌及痢疾杆菌)互相接合传递的规律及表达。

The fragment was connected to pcDNA3.0 carrier which was cut by the same two enzymes to form the plasmid which was transformed into Escherichia coli DH5a.Appropriate clone was cultured to extract plasmid.The extract was analysed to confirm that it was constructed correctively.

将该片段连接到同样被KpnⅠ和XhoⅠ双酶切的pcDNA3.0载体中，转化大肠杆菌DH5α感受态细菌，挑取阳性克隆进行质粒提取，进行酶切鉴定，并获得阳性克隆。

MAIN OUTCOME MEASURES: The results of recombinant plasmid PCR and gene sequencing, the correlation coefficient of the standard curve and the repeatability of the plasmid standard.

主要观察指标：重组质粒聚合酶链反应和基因测序结果，标准品标准曲线的相关系数及标准品的重复性。

The results showed that plasmid DNA density was correlation to the ratio of seedset significantly but gene type and transformation time was not.

结果表明：不同基因型、转化时间对结实率影响不显著，质粒DNA浓度对结实率影响显著。

The nucleus of the transfected cells were crenation or broken down into pieces, which proved that ERIP1 could induce apoptosis.

在免疫组织化学实验中，将带有GFP标签的ERIP1转染入HEK-293T和MCF-7细胞，发现细胞大面积死亡，细胞中转染入质粒后，细胞核皱缩、破碎，说明ERIP1可能诱导细胞凋亡。

Results: Six standard serotype strains of CT were detected specifically by Plasmid Gap-LCR-ELISA with no cross-reaction to the other bacteria.

质粒Gap-LCR-ELISA对检测CT感染具有极高的灵敏度和特异度，适宜于我国进行大规模CT流行病学调查。

This causes crown gall tumors in plants, and its plasmid has been used on a range of crop plants.

这一质粒已在一系列的农作物中得到了应用。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！