质粒

ResultsUsing this method, we successfully cloned the 11kb virB operon of Brucella and constructed complementary plasmid.

我们利用该方法克隆了布鲁氏菌中长度为11kb的virB操纵子，并构建了互补质粒。

Using pUC19-SacB, we successfully constructed an unmarked deletion mutant of the VirB operon, showing that this plasmid can be efficiently used to modify the Brucella chromosome.

我们利用该质粒，成功的构建了布鲁氏菌Ⅳ型分泌系统的突变株，改建的载体能高效的应用于布鲁氏菌染色体的精确修饰。

By 42 days postvaccination, pigs in group 1 and group 2 had seroconverted to PCV2 capsid antibody.

在免疫后42天，PCV1-2组及真核表达质粒组产生了PCV2抗体。

The recombiant protein with GST tag was soluble. SjMRLC showed similar transcription level in all stages including sporocyst, miracidium, egg, cercaria, schistosomulum, female worm, male worm, and paired adults.

结果 构建pGEX 4T-3/SjMRLC重组质粒，测序表明插入的外源基因片段正确，诱导表达获得带有GST标签的可溶性蛋白（Mr 49 000）；虫期转录特异性分析显示该基因在日本血吸虫各个虫期均有转录，且水平一致。

To study and know well the rudimentary knowledge and the basic techniques of modern Microbiology are demanded for Graduate students. The experiments including the principle and operation of phase contrast microscope and fluorescence microscope; Microphotography , the technique of photographic enlargement;Applications of polymerase chain reaction ; Construction, transformation and detection of plasmid in E.coli ; Infectivity of virus and its molecular detection ; The technique of protoplast fusion in filamentary fungi ; Induction and purification of β-galactosidase from E.coli and assay the enzyme activity; Detection of GC content and DNA-DNA hybridization in bacteria ; Production , extraction and detection of poly--hyduoxy butyrate from bacteria.

以实验教学为主，要求研究生学习和掌握一些现代微生物学研究所需的基本技术，内容包括：相差显微镜、荧光显微镜的使用和显微摄影技术，PCR技术的应用，细菌质粒的构建、转化及检测技术，病毒的侵染性及其分子检测技术，丝状真菌原生质体融合技术，大肠杆菌β-半乳糖苷酶的分离及纯化及其动力学测定技术，细菌G+C mol%的测定及细菌DNA-DNA杂交技术和聚羟基丁酸产生菌的发酵、产物提取及检测技术等。

Strain MCB032 which has a large plasmid can grow with chlorobenzene as the sole carbon source.

中文摘要许多微生物能够降解异源物质，而且这些降解作用的酶通常编码在质粒上。

In our study, systematically investigated the effect of chloroquine on exogenous gene expressing in mice by hydrodynamics-based naked plasmid adminstration was reported.

我们首先系统的研究了氯喹对裸质粒介导的人凝血Ⅸ因子在小鼠体内表达的影响。

GFP and the control chymotrypsin dsRNA and only the transfection of pAct.

GFP质粒的细胞及仅转染pAct。

The amplified full-length plasmid PCR products were transformed into competent E. coli and they were able to circularize due to the terminal homologous sequences.

转化到细菌中的全长质粒DNA PCR产物可利用其末端同源序列发生同源重组而环化。

Methods The plasmid expressing green fluorescent protein was used as the reporter gene, and the chitosan-pGFP NPs were prepared using a complex coacervation process.

用表达绿色荧光蛋白的质粒作报告基因，采用复凝聚法制备壳聚糖－pGFP纳米粒。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！