质粒

Expression and Beat Frequency in Rat Myocardial Cells

质粒介导shRNA对心肌细胞kir2.1蛋白表达和搏动频率的影响

Methods Cadmium-specific promoter PcadA was obtained by PCR from pPcadA and was ligated to E.coli-B.subtilis shuttle vector pNGFP with gfp being reporter gene ,after which the recombinant plasmid was transformed into 1A751 strain to form the bio-engineered bacteria.

采用聚合酶链式反应从质粒pPcadA上扩增得到重金属镉离子特异性启动子PcadA，以绿色荧光蛋白基因为报告基因，以大肠杆菌-枯草杆菌穿梭载体pNGFP构建了绿色荧光蛋白镉离子诱导表达载体。

METHODS To analyze its biotype,antibioticgram,serotype,plasmidgram and their relationship combining the clinical test examination.

方法结合检验工作，先后用生物分型、抗菌谱分型、血清分型和质粒图谱分型，再交互分析其关系。

Objective: To Construct and identify the bivalent recombinant baculovirus transfer plasmid of HPV type 16 and type 11 gene.

目的：构建人乳头瘤病毒16型与11型L1基因双价重组杆状病毒转移质粒并对其进行鉴定。

The tetraploid embryos were obtained from diploid blastomere electrofusion which preformed at 2-cell stage.

通过电穿孔的方法将增强型绿色荧光蛋白表达质粒转入ESCs，用G418筛选获得EGFP-ESCs。

Objective To construct a prokaryotic expression vector encoding first 193 amino acids of BPI N terminal.

目的：建立杀菌/渗透增强蛋白 N 端193个氨基酸的重组表达质粒。

Objective To construct a prokaryotic expression vector encoding first 193 amino acids of BPI Nterminal.

目的：建立杀菌/渗透增强蛋白 N端193个氨基酸的重组表达质粒。

Results A 579 bp fragment was amplified by RTPCR. BPI cDNA was inserted into pET28a.

结果：从HL60细胞中扩增得到579 bp的BPI 193基因片段，构建了TBPI 193亚克隆和PETBPI 193重组表达质粒。

In this report, we constructed a new suicide plasmid to construct Brucella mutant.

本研究构建了一个可用于布鲁氏菌突变株构建的自杀质粒。

Using this plasmid, we constructed replacement mutant of Brucella omp25 gene.

利用该质粒，我们构建了布鲁氏菌外膜蛋白Omp25基因的突变株。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！