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Some factors were found to affect the expression of aguA in E.

得到一系列突变基因并将它们成功的插入到表达载体pTrc99A和pHsh中得到重组质粒

In this review, the application of anion exchange chromatography to pharmaceutical plasmid analysis was reviewed.

本文对阴离子交换色谱在药用质粒分析方面的应用与研究进展进行综述。

Recombinant plasmid pFf-22-Trp operon was successfully constructed, and both the anthranilate synthase and tryptophan synthase activities of expressed product increased. It laid a foundation of construction of recombinant E. coli strain for production of tryptophan in a large scale.

已成功构建了重组表达质粒pET-22b-Trp operon,邻氨基苯甲酸合成酶和色氨酸合成酶的活性在大肠杆菌中得到了提高,为高产色氨酸基因工程菌的构建奠定了基础。

ThyA. ctxB was also cloned in this plasmid to elicit anti-toxin immunal response. This improved vaccine candidate derived from IEM101 is named IEM108.which can elicit both antibacterial and antitoxic immunity.

并采用以管家基因thyA为选择压力的质粒-染色体致死平衡系统使之稳定表达,构建生物安全的抗菌抗毒基因工程活菌苗,该菌苗株命名为IEM108。

But the material particle DNA sharp separation purification to the ultra centrifugal equipment (supercentrifuge, turned the head with appurtenance) to set a higher request.

质粒DNA的快速分离纯化又对超离心设备(超速离心机、转头和附属设备)提出了更高要求。

Objective: To express rALR in GS115 with the constructed pichia pastoris expression plasmid of rat augmenter of liver regeneration and purify it with ultrafiltration and identify its bioactivity in vitro, for studying its biological functions.

目的:利用已构建的大鼠肝再生增强因子毕赤酵母重组表达质粒pPIC9K-rALR,在酵母菌GS115中诱导表达rALR,并进行纯化和体外生物学活性鉴定,为进一步研究rALR的生物学功能提供基础。

Azole resistance was found in the candida albicans isolate from the renal transplant recipients, and there is no connection between incidence of resistance and isolate locus.

通过PCR扩增该基因上游启动子调控序列,转化质粒扩增后测序未找到耐药/敏感菌株在调控序列上的任何点突变。

Instead of plasmids the strong bacteriophage T5 promoter has been used to replace native promoters in E.

而非质粒强烈噬菌体T5的启动子已被用来取代本土启动子在大肠杆菌中。

Used of a bacteriophage or plasmid.

用于噬菌体或质粒

Successful construction and expression of PEA in prokaryotic expression vector are basis for further study of genetic engineering vaccine and recombinant inununotoxin for immune target therapy.

PET-32a-PEA原核表达质粒的成功构建及表达,为进一步研究有效的基因工程疫苗和免疫导向治疗的基因重组毒素奠定了基础。

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