质粒

The omp17.3 gene of Brucella abortus was amplified by PCR and then the amplicon was cloned into the eukaryotic expression plasmid pcDNA3.1 to construct a recombinant plasmid pcDNA3.1-omp17.3. Then the recombinant plasmid pcDNA3.1-omp17.3 was transfected into COS7 cells, and the expressed OMP 17.3 was detected by Western-blotting.

采用PCR方法扩增了布鲁氏菌17.3ku外膜蛋白编码基因，并将该基因克隆至真核表达载体pcDNA3.1中，成功构建了真核表达质粒pcDNA3.1-omp17.3.pcDNA3.1-omp17.3转染COS-7细胞后，通过Western-blotting检测到了17.3ku蛋白的瞬时表达。

Recombinant colony, E.coli DH5α(pMG320),expressing O\|antigen of V.cholerae O 139 was detected from the bank by immunological agglutinative reaction.

经鉴定分析重组克隆所表达的O 抗原具有良好的免疫原性及反应原性。酶切分析质粒 pMG32 0 ，推知其O 抗原基因大小约 4 6kb。

In vitro associstion experiments indicate that the plasmid pAy6.8 containing the core region of Antheraea yamamai fibroin gene can tightly bind to the nuclear matrix of Bombyx mori fertilized eggs,and recognize the same binding sites of homogeoous MAR fragments.

体外结合实验表明，携带天蚕丝素基因核心区的质粒pAy6.8可与家蚕受精卵核基质紧密结合，而且同内源MAR片段竞争结合位点。

METHODS Human IL 6 gene was reconstructed in retrovirus vector and transferred into incasing cells PA317 by lipofectamine mediated method. The clones of the cells transferred with hIL 6 were selected by G418. Targeted NIH3T3 cells were infected with the virus granules secreted from PA317 and also selected by G418. The insertion and expression of hIL 6 gene in NIH3T3 cells were analysed with Southern blot and Northern blot. RESULTS Human IL 6 retrovirus vector (pZIPIL-6) was successfully reconstructed.

利用重组载体构建技术将质粒pUCIL 6 cDNA的目的片段连接于逆转录病毒载体上，并以脂质体介导的方法将重组载体转染包装细胞PA317，以G418筛选克隆细胞，浓缩克隆细胞上清以制备重组病毒液，继之感染NIH3T3细胞后，进行Southern blot和Northern blot分析，检测目的基因在靶细胞的整合与转录水平。

The inducible type isolations and their constitutive type changers were put into the same group.

对每组细菌的质粒ampC基因和染色质ampD基因进行扩增、测序和序列比对。

Results: it was efficient to transfect chicken embryonic fibroblast cells by the method of lipofectamine mediation. the expression levels of protein were significantly different.

结果 脂质体介导的方法能有效转染鸡胚胎成纤维细胞，实验中egfp蛋白相对表达量随质粒量的增加而明显升高（p.01）。

OBJECTIVE: To transfect rabbit bone marrow mesenchymal stem cells using pIRES2-EGFP-BMP-2 eukaryotic expression plasmid.

目的：使用前期已经构建成功的pIRES2-EGFP-BMP-2真核表达质粒转染兔骨髓间充质干细胞。

The plasmid DNA remained intact and could be stored at 4℃ for at least one month in liposomes, it was also resistent to DNase I digestion.

重组质粒DNA在脂质体的制备过程中保持了稳定性，并能抵抗DNA酶的水解破坏作用。

AIM: To construct the eukaryotic expressing vector of human cbl and test its expression in African green monkey kidney cell line COS 7. METHODS: Human cbl gene tagged with flag was amplified from pEFHAcbl plasmid by PCR. The product was cloned into pGEM T easy, sequenced and then subcloned into eukaryotic expressing vector pcDNA3.1.

以含有人全长cbl cDNA的质粒pEFHAcbl 为模板，采用PCR方法扩增cbl基因，并在其N 末端带上含24 bp的flag标签，克隆到pGEM T easy载体并测序，再亚克隆至真核表达载体pcDNA3 1，酶切鉴定正确后采用脂质体法瞬时转染COS 7细胞，Western blot检测flag cbl在细胞中的表达。

METHODS: Human cbl gene tagged with flag was amplified from pEFHAcbl plasmid by PCR. The product was cAIM: To construct the eukaryotic expressing vector of human cbl and test its expression in African green monkey kidney cell line COS7. METHODS: Human cbl gene tagged with flag was amplified from pEFHAcbl plasmid by PCR. The product was cloned into pGEMT easy, sequenced and then subcloned into eukaryotic expressing vector pcDNA3.1.

以含有人全长cbl cDNA的质粒pEFHAcbl 为模板，采用PCR方法扩增cbl基因，并在其N末端带上含24 bp的flag标签，克隆到pGEMT easy载体并测序，再亚克隆至真核表达载体pcDNA31，酶切鉴定正确后采用脂质体法瞬时转染COS7细胞，Western blot检测flagcbl在细胞中的表达。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！