质粒

However, 2 inserted fragments of recombined plasmids have no overlapped regions. Further more, when these recombined plasmids were retransformted to M145::SCE25AT, no Km~sApr~R and "bald" phenotype transformant were obtained in 5,000 Thio~R transformants.

但是，测定2个重组质粒的插入片段没有发现相互重叠区；而且，提取2株重组质粒重转化到M145：：SCE25AT中，分别得到5，000株Thio~R转化子，但没有得到Km~SApr~R及&光秃&表型的转化子。

The recombinant plasmid DNA of PrM-E gene but not unmethylated CpG was capable of inducting antibodies against D2-43 virus.

我国登革2型病毒PrM-E基因重组质粒DNA具有一定的免疫原性。在本实验条件下，含非甲基化细菌性CpG的pUC19质粒DNA，对PrM-E基因的DNA免疫效果没有促进作用。

Results :The essay had amplified fragment of gene from 20 white colons, and sequenced the DNA.

结果：实验从20个白色菌落中经PCR扩增出基因片段，对其中6个克隆的质粒进行DNA序列测定，发现其中1个质粒含有特定的人hnRNP A2／Bl基因序列。

DNA immunization with the Sj16 gene Plasmid DNA was prepared by QIAGEN plasmid mega kit and redissolved in NS at a final concentration of 1μg/μl.

Sj16基因DNA免疫研究用QIAGEN Plasmid mega大量质粒提试剂盒大量制备高纯度的pcDNA〓-Sj16、pcDNA〓和pUC19质粒DNA。

To test this hypothesis, we performed experiments using plasmids containing a luciferase reporter with amber, ochre and opal nonsense mutations within the luxB gene in Escherichia coli.

为验证此假设，我们用几个含有大肠杆菌荧光素酶报告基因的质粒进行了实验，这些质粒中的luxB基因分别包含有琥珀色、赭色和乳白色的无义突变。

The fragment was then cloned into the unique Xba Ⅰ site of the transfer vector pVL1393 by blunt-ended ligation.

目的基因片段和表达质粒载体pVL1393片段进行钝端连接，获得表达质粒载体pVL1393-HμOR-6His。

The optimal conditions for electroporation transformation of Pichia Pastoris were obtained with the maximum transformation efficiency of 36 transformants/μg plasmid DNA when the intermediate logarithmic phase yeast cells were used for competent cells, and the electroporations were under the conditions of voltage of 1.5kV and 0.15mg / mL plasmid DNA and 0.2 cm cuvettes.

结果表明：当采用对数生长中期的菌体制备感受态细胞、电压为 1.5kV、质粒浓度为 0.15μg/μL和 0.2 cm 电转化杯时，转化效率达到最大值，为每微克质粒 DNA 36个转化子。

A recombinant pl asmid (designated as p8.0) was obtaining by ligating these three fragments. The p8.0 was subcloned into a plasmid (designated as p8.2) containing entire LTR of EIAV DLA. The complete nucleotide sequence of DLA strain of EIAV was determined by sequencing the p 8.2 . The purified p8.2 was used to transfect donkey leuko cyte cultures.

将此8.0kb EIAV全基因再亚克隆到含有一完整EIAV DLA株长末端重复序列的质粒中，获得一含有EIAV驴白细胞弱毒前病毒全基因的重组质粒，将其命名为p8.2，经核苷酸序列分析，证明p8.2含有EIAV前病毒的全基因。

DNA efficiency and quality can be analysed by agarose gel electrophoresis and double enzymatic digestions.

结果 C、D法提纯的质粒DNA的含量、纯度和收获率均较A、B高，上述4 方法提纯的质粒DNA都能进行酶切反应。

Dna efficiency and quality can be analysed by agarose gel electrophoresis and double enzymatic digestions.

结果 c、d法提纯的质粒dna的含量、纯度和收获率均较a、b高，上述4种方法提纯的质粒dna都能进行酶切反应。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！