质粒

The detection rate of DHA-l is much higher than that in other regions; Pa isolated with DHA-1 gene showed powerful activities with resistance to cephalosporin and monocyclic lactam antibiotics. The prevalence of Pa in the same or different departments should be noted seriously.

淮北矿工总医院DHA-1型质粒AmpC酶检出率远高于国内其他地区；产DHA-1型质粒AmpC酶菌株对头孢菌素及单环酰胺类抗生素耐药严重，在同一科室或不同科室间的传播应引起高度重视。

To construct a plasmid for analyzing gene functions and expressions and to study the MXAN1334 gene in Myxococcus xanthus with the plasmid.

旨在构建一个用于基因插入失活，并同时可通过报告基因分析插入位点基因表达情况的质粒载体，并应用该质粒对黄色粘球菌MXAN1334基因功能和表达情况进行分析。

It was observed that although the pSym-curred strain HND29SR could not nodulate on the host plant Heinong 33, there was no difference in the root colonization dynamics and density of rhizobium population between HND29SR and its wild-type HN01 or reference strain HN01L in sterilized pot-sand system.

这一现象说明HN01所含共生大质粒不影响其根圈定殖行为，有理由推测影响HN01根圈定殖行为的有关基因可能存在于染色体上或另外两个内源质粒上。

The resulting strain, designated YES2MTM1, was transformed with a yeast genomic library. Transformants lost the plasmid overexpressing MTM1 after 5-FOA treatment. Yeast strains able to grow on nonfermentable carbon source with MTM1 deletion and overexpression of some DNA fragments were picked up and candidate suppressor genes were identified. Overexpression of five genes were identified to be able to rescue the growth defect on nonfermentable carbon source. The study will provide reference for MTM1 gene function and screening for suppressor of genes whose deletion result in irreversible damage.

为了避免MTM1缺失造成的不可逆损伤，在野生型酵母中先转入带有MTM1 基因的质粒，再敲除染色体上的MTM1 基因，随后转入基因组文库，再利用药物5-氟乳清酸(5-FOA)迫使细胞丢失表达MTM1基因的外源质粒，再筛选能在非发酵培养基上生长的转化子，通过这种方法筛选发现，POR2等5个基因的过表达可以挽救MTM1 基因缺失造成的非发酵培养基上的生长缺陷，为深入了解MTM1基因的功能提供了线索，对筛选其他造成不可逆损伤的突变基因的抑制基因提供了一条可行的研究思路。

After being digested with EcoR I and Xho I, they were inserted to the vectors pGEX-4T-l orientally, and then transformed into E.coli BL21(DE3) cells. The monoclone were selected and identified by means of restriction analysis, PCR and sequencing. As the result, the two recombinant plasmids with the different DNA fragements of the PrP gene were constructed and designated as pMY01[Ov rPrP(23-244)] and pMY02[Ov rPrP(91 -244)], respectively.

挑选单个菌落，提取重组质粒，经酶切、PCR扩增后琼脂糖凝胶电泳分析和测序鉴定，成功地构建了2种绵羊重组朊蛋白的表达质粒pMY01[OvrPrP(23～244)]和pMYO2[Ov rPrP(91～244)]。

In this study, the structure sequences of Shiga toxin 2B(stx2B) and Shiga toxin 1B(stx1B) were respectively amplified from EHEC O157:H7 by PCR. stx2B-L-stx1B fusion gene was constructed by a 12 bp linker ligating these two fragments. After digested with restriction endonuclease Nco Ⅰ and EcoR Ⅰ, the fusion gene was orientally inserted into expression vector pET28a.

利用PCR方法，从肠出血性大肠杆菌O157：H7的基因组DNA中，分别扩增出Stx1B和Stx2B亚基的结构基因片段，然后再通过PCR将这2个片段连接起来，并定向克隆到表达质粒pET28a的NcoⅠ和EcoRⅠ位点之间，构建了重组表达质粒pMB1。

Objective To investigate the possibility of self-assembling gene nanoparticles between the amphiphilic chitosan derivative and plasmid DNA, and study the interaction mechanism between N -methylene phosphonic chitosan and plasmid DNA, which might provide a new strategy for developing safe and efficient non-viral vectors.

目的 探讨双亲性壳聚糖衍生物与质粒DNA通过自组装的方式形成基因纳米粒子的可行性，考察载体与质粒DNA的相互作用机制，为开发安全高效的非病毒载体提供新的途径。

The phytopathogenic fungus Fusarium graminearum has been transformedusing a plasmid (pAN7-1) containing the Escherichia coli hygromycinphosphotransferase gene. Stable hygromycin-resistant transformant coloniesappeared at frequencies between 2 and 10 per μg plasmid DNA.

将含有大肠杆菌中潮霉素B磷酸转移酶控制基因的质粒pAN7-1转化入小麦赤霉病菌野生菌株中，结果表明，pAN7-1的转化效率为2-10个/μg质粒DNA。

The chromatin unfolding assay showed that ,like the wild-type transactivation domain, two variants that represent benign polymorphisms did not induce chromatin unfolding or only induced subtle change. Contrary to the behaviors of the wild type and two benign variants, four cancer-predisposing mutations in the transactivation domain superactivate the chromatin unfolding. The results suggest that the chromatin unfolding assay can aid in the characterization of deleterious mutations in the C-terminal transactivation domain of BRCA1 and may provide more reliable presymptomatic risk assessment.

对这些重组质粒的染色质伸展活性检测表明，野生型pwt和两种良性多态性突变体不具有染色质伸展活性或只有极微弱的染色质伸展活性，而其他4种乳腺癌易感突变体均具有过强的染色质伸展活性，提示利用染色质伸展技术可预测BRCA1转录激活区基因型与乳腺癌发生风险的表现型的关系。

The plasmid pAO815 is then used in homologous recombination, and the recombinant plasmid pETnsGH is further converted, jointed and inserted into GS115 saccharomycetes for the foreign gene to express normally.

再选择质粒pAO815进行同源重组，进而有效地将重组质粒pETnsGH转化接合插入GS115菌种中，使外源基因sGH正常表达。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！