质粒

The percentage of plasmid in vibrio cholerae from different countries was reviewed and the biological characteristics were introduced .

本文对国外报道的霍乱弧菌中的质粒携带率以及质粒决定的生物学功能进行综述。

The invention discloses a pyruvate oxidase gene derived from viridian aerococcus, recombinant expression plasmid constructed by the gene and an engineering strain of escherichia coli gene transformed by the recombinant expression plasmid.

本发明公开了一种绿色气球菌来源的丙酮酸氧化酶基因AvPyOD，该基因所构建的重组表达质粒，以及该重组表达质粒转化的大肠杆菌基因工程菌株。

To study the molecular structure and function of the PyNPase, and to construct a system for preparation of the enzyme for practical synthesis of nucleoside analogs, molecular cloning of the encoding the PyNPase from E. aerogenes strain EAM-Z〓 have now been completed.

我们通过以该酶的N端氨基酸序列设计上游引物，以不同来源PyNPase的C末端同源区氨基酸序列设计下游引物，采用PCR技术，扩增出该酶基因的编码区（约1206bp），并完成了其重组质粒的构建和核苷酸序列的测定，目前正在进行PyNPase表达质粒的构建。

In accordance with the present invention, optically pure-hydroxycarboxylic acid can be prepared by culturing E. coli transformed with a recombinant plasmid which expresses intracellular PHA depolymerase of Ralstonia eutropha and PHA biosynthesis enzyme of Alcaligenes latus to give-hydroxycarboxylic acid.

根据本发明，光学纯的-羟基羧酸可通过培养用重组质粒转化的E.coli制备，所述重组质粒表达真养产碱杆菌的胞内PHA解聚酶和广泛产碱菌的PHA生物合成酶从而生成-羟基羧酸。

A malignant transformant line 3T3 (T24) was obtained by transfection of mouse fibroblast NIH3T3 cells with pMAMneo-T24-ras plasmid. 3T3 (T24) cells were cultured continuously in the presence of benzamide , an NAD site inhibitor of poly polymerase .

将12位密码子突变的活化ras癌基因全cDNA序列克隆到真核表达载体pSV2neo和pMAMneo上，获得了相应的重组质粒pSV2neo-T24-ras和pMAMneo-T24-ras，将pMAMneo-T24-ras质粒利用基因转移方法导入小鼠成纤维细胞NIH3T3中，活化的ras癌基因可使受体细胞发生恶性转化。

An expression vector for one isoform of the mbC α1 subunit cDNA was constructed using the human cytomegalovirus promoter to direct expression and this expression vector was used in a novel transfection assay of primary cultures of bovine adrenal chromaffin cells.

为表达具有生物活性的钙离子通道，将mbC的一个异构体的蛋白编码区插入到由人的涎腺病毒启动子控制表达的质粒中。这个表达质粒

After 10 weeks immunization, the combinant plasmids distribute different organs and tissues of the BALB/c mice immunized by DNA vaccines of Toxoplasma gondii ,but only blood was Detected out the recombinant plasmids.

结论弓形虫DNA疫苗免疫BALB／c鼠5周后，多个不同器官组织均可检测出重组质粒，免疫后10周血液检测出重组质粒。

The GPA1 gene was obtained via PCR amplification and was cloned in the two-hybrid DNA binding domain vector pGBKT7 The combinant plasmid was designated as pGBKT7-Ga.β-galactosidase assay indicatedthat Got did not have the property of self-activation. After pGBKT7-Ga was transformed to yeast pJ69-4A, we transformed Arabidopsis vegetative tissue two-hybrid cDNA library plasmids to yeast pJ69-4A containing pGBKT7-Ga.

通过PCR扩增得到GPA1基因并将其克隆到双杂交DNA结合域载体pGBKT7中，得到的重组质粒命名为pGBKT7-Gα，β—半乳糖苷酶活性鉴定表明Gα不具有自激活特性，将其转化到酵母菌pJ69-4A中，再以此为受体菌转化拟南芥绿色营养组织cDNA文库质粒。

Methods Agarose gel electrophoresis analysis was used to detect plasmid profiles. Plate transconjugation and broth transconjugation were used to examine the conjugative resistant plasmids and the agar plate dilution method was used to analyze the minimum inhibitory concentration of 15 antimicrobial agents.

用改良Kado-Liu法提取细菌质粒，琼脂糖凝胶电泳检测质粒，用平板稀释法测定药物最低抑菌浓度，用试管内肉汤接合法和平板表面接合法进行接合转移试验。

In order to do this, we get equal quantities of plasmid pSCA1-M, pcDNAM and pSCA1 transfected vero cells. After 48 hours, we observed the presence of special green fluorescence in cytolymph in both of pSCA1-M and pcDNAM groups. However, this fluorescence is absent in the negative pSCA1 group.

分别取等量的重组质粒pSCA1-M、常规IBV M基因DNA疫苗质粒pcDNAM、空载体pSCA1转染Veto细胞。48h后经间接免疫荧光试验观察发现，pSCA1-M以及pcDNAM转染组细胞浆均可见特异性亮绿色荧光，pSCA1空载体组无荧光为阴性。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！