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Results:Expression of elastase mRNA has been found in the endothelial cells,the medial smooth muscle cells and the adventitial fibroblasts of the abdominal aorta,the lymphocytes,monocytes in blood,the tracheal hyaline cartilaginous cells,the glandular cells of the pancreas,the epithelial cells of the parotid gland and submaxillary gland,the hepatoeytes,the endothelial cells of the liver sinusoid wall,the goblet cells of the mucous membrane of the small intestine,the cardiac myocytes,the renal interstitial fibroblasts,the alveolar epithelial cells,the cerebral glial cells,the fibroblasts of the dermis oorium of the skin,the primary spermaocytes,the secondary spermaocytes and sperm in the seminfferous tubule of the testis,the lymphocytes in the spleen and thymus.

结果正常大鼠腹主动脉的内皮细胞、中膜平滑肌细胞以及血管外膜成纤维细胞,血液细胞中的淋巴细胞、单核细胞,气管透明软骨细胞,胰腺的腺细胞、腮腺、颔下腺上皮细胞,肝细胞、肝窦壁的内皮细胞,小肠黏膜杯状细胞,心肌细胞,肾间质的纤维母细胞,肺泡上皮细胞,大脑胶质细胞,皮肤真皮纤维母细胞,睾丸曲精细管内的初级精母细胞、次级精母细胞以及精子,脾脏以及胸腺的淋巴细胞等,均有弹力蛋白酶mRNA的表达。

These two series of probes differ in their mechanism of action. The first series exploits the concept of suicide substrate. When the probe is hydrolyzed by the target sulfatase, it will release an intermediate that undergoes elimination to form a reactive quinone methide which in turn reacts with nearby nucleophiles. The second series belongs to an electrophilic reagent which utilizes a cyclic sulfamate moiety serving both as the recognition unit and the trapping device.

这二系列标示分子的主要差异在於其标示机制,第一系列是源自於自杀性受质的概念,利用水解后的中间体会生成高反应性的quinone methide,来达到标示的效果;第二系列则是属於亲核性试剂型,采用环状胺基磺酸衍生物做为辨识端及捕捉机制,预期在其进入酵素的受质结合区后能与酵素催化中心形成共价键结。

This result suggests that cytomixis occurred at synizesis stage may produce some kind of stress which results in activation of retrotransposon to insert into a new locus, or/and breakage rearrangement at the site of retrotransposon in genomic DNA, that both can be detected by fingerprinning.

分析认为在兰州百合减数分裂前期Ⅰ的凝线期时,由于染色质的胞间运动造成对基因组DNA的胁迫,导致了逆转座子del的激活,所插入的位点序列即为所检测到的特异片段;或者是由于染色质的胞间运动造成染色体的断裂重排。

The concentrations of CODcr and NH4+ vary from low to high, then from high to low in the process of refuse leaching, that is to say that there are three phases which are aerobian decomposition, facultative aerobian and anaerobian decomposition;(3)The time of landfill reaching to stabilize under the condition of filtration, for landfill of Jinqianpu, is at least 86 years if CODcr is regarded as a index; but is about 98 years when NH4+ as an index;(4)The column leaching experiment simulated is carried on interactivity between leachate and different lithologic characters, water-bearing media of different thickness. Some parameters can be determined by calculation, such as, the retained factor and hydro-chemical transportation rate of pollutants in suspended media, which quantitatively analyse the suspended soil capability of components purification and the pollution scope of water bearing media.

垃圾淋滤过程中和经历了一个由低到高、再由高到低的过程,即垃圾填埋过程中经历了好氧分解、兼性好氧分解及完全厌氧分解等三个阶段;(3)垃圾组分在淋滤条件下分解达到稳定的时间,对于金钱堡垃圾填埋场,若以作为考核指标,填埋场达到稳定化时间至少需要86年;若以作为考核指标,则填埋场达到稳定化至少需98年的时间;(4)对垃圾浸泡液与不同岩性、不同厚度含水层介质之间的作用进行了土柱淋滤模拟试验,通过计算污染质在包气带介质中运移的阻滞因子和水化学迁移率等参数,定量地描述了包气带土层对污染质的净化能力和污染范围。

In the1990s.go with the tide of economic golbalization,French cosmetics Danli series beganin the famous global beauty salon for sale in the Pearl of the Orient Shanghai,set up specifically for Asian skin on the study of Danli Center and introduced for the Asian skin cosmetics,so that the vast majority of the world's Oriental women have begun to have their own private skin expert.

上世纪九十年代,顺应全球化经济浪潮,法丹莉系列化妆品开始在全球各著名的美容沙龙内售卖,在东方之珠的上海,更成立了专为东方人肤质研究的法丹莉研究中心,并推出了针对东方人肤质的美容化妆品,从而让占全球绝大多数的东方女性也开始拥有了自己的私人皮肤问题专家。

The result showed that, the body wall most outer layer cuticle assumesthe translucent porodine, the surrounding entire body wall; Close isconnected with the cuticle 表皮层 for 合胞体 the structure, inthe back, the abdomen and its two sides separately adds thick to inprominent Cheng Jizhuang, separately contains the back side,腹侧the nerve and compares to the developed back, the abdomen nerve; Themyo- position to the body wall most inner layer, has the muscle cellto arrange becomes; Between the body wall and the digestive tractcavity is a body cavity; Digestive tract for 肌质 structure, afterinference for roundworm's esophagus.

结果显示,体壁最外层的角质层呈半透明的胶状,包围整个体壁;与角质层紧密相连的表皮层为为合胞体构造,在背、腹及其两个侧面分别加厚向内突出成脊状,分别包含背侧、腹侧神经和相比对发达的背、腹神经;肌层位于体壁最内层,有肌细胞排列而成;体壁与消化道之间的空腔为体腔;消化道为肌质结构,经推断为蛔虫的食道。

Moreover,it was demonstrated that the threesubstances,E,DE and DA,increased dramatically and quickly after LPS treatment.Also,it was revealed that the changes of three substances were more quickly than thechanges of the above mentioned APPs during the same LPS treatment.Thesesuggested that the three substances existed as neurotransmitter.The presence and thechange of the CAs in amphioxus after treated with LPS indicated the evolutionaryexistence of CAs and acute phase response in the protochordate lineage.

第四,我们用高效液相色谱法(High Performance Liquid Chromatography,HPLC),探讨了LPS感染文昌鱼儿茶酚胺类神经递质的变化,优化了同时测定文昌鱼体液中NE、E和DA三种CAs的HPLC方法,并首次发现,文昌鱼体液中同时存在NE、E、和DA三种CAs物质,其中,E在文昌鱼中首次报道,这三种物质在LPS感染文昌鱼后浓度升高非常明显,与同期的APPs相比变化更为迅速,也体现了神经递质的主导作用,文昌鱼的CAs系统也具有了类似脊椎动物CAs系统的功能,可以初步判断CAs系统的进化源头由脊椎动物可推回到头索类。

Continuous flow experiment was carried out using acidogenic-desulfate bioreactor and it is suggested that on quality,SRBis the hydrogen consumer and the remaker of the end products of AB;and maintains the typical characteristic of"acetate-type" metabolism of its population. On quantity, SRB maintains dynamic balance between"substrate chain"and"bio-chain",describing the dynamic of its population.

采用产酸脱硫反应器进行连续流试验,提出在&质&的方面,SRB是产酸脱硫生态系统中氢的&消费者&和产酸菌酸性末端产物的&改造者&,并维持着群落&乙酸型代谢&的典型特征;在&量&的方面,SRB种群通过数量波动维持着&基质链&与&生物链&的动态平衡,并以种群动态为表征。

Continuous flow experiment was carried out using acidogenic-desulfate bioreactor and it is suggested that on quality,SRBis the hydrogen consumer and the remaker of the end products of AB;and maintains the typical characteristic of"acetate-type" metabolism of its population. On quantity, SRB maintains dynamic balance between"substrate chain"and"bio-chain",describing the dynamic of its population.

摘 要:采用产酸脱硫反应器进行连续流试验,提出在&质&的方面,SRB是产酸脱硫生态系统中氢的&消费者&和产酸菌酸性末端产物的&改造者&,并维持着群落&乙酸型代谢&的典型特征;在&量&的方面,SRB种群通过数量波动维持着&基质链&与&生物链&的动态平衡,并以种群动态为表征。

Methods Employing cholera toxin B-subunit combined horseradish peroxidase retrograde tracing technique and cholera toxin B-subunit combined colloid goldretrograde tracing with calcitonin gene-related peptideimmunohistochemical double-labeled staining technique,the innervation of musculus sacrococcygeus ventralis medialisin rats was studied.

大鼠骶尾腹内侧肌又称荐尾腹侧中肌(musculus sacrococcygeus ventralis medialis)或屈尾短肌,是位于盆腔后壁、骶椎前方的一对梭形肌肉,属尾肌群,因其功能似乎仅为屈尾,故至今尚未引起人们的注意,而与该肌位置关系较密切的会阴肌因在某些运动神经元疾患时仍可保持其正常生理功能而一直受到神经工作者的关注,尤其是近年来有诸多文献报道了会阴肌的神经支配、相关神经元的细胞构筑、神经递质和神经调质的分布以及纤维联系特点[1~10],藉以探讨运动神经元疾病的神经病理学。

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