质次的

This type is accompanied by the structure-salt dissolution breccias,being vein or flamboyancy in cross section while some of them shot through the lead and zinc ore. In fact,the breccias were lead and zinc orebodies themselves.

其角砾成分单一(主要为沥青质灰岩、晶质灰岩)、大小混杂(nmm～nX10m)、无分选，角砾多呈不规则状和次棱角状或次浑圆状，部分角砾具有可拼性，角砾间为砂质充填或无充填，表明它是一套构造和膏溶共同作用形成的角砾岩。

In every group, rabbits were subdivided into experimental and control subgroups. 2 Rabbits were bullets injected followed by continuous injected with 13C labeled leucine, glucose, and lactic acid; 3 Blood were drewed before and 150, 160, 170, and 180 min after the initiation of isotopes injection for material analysis; 4 Exhale gas were collected every 5 min in the first 30 min followed by every 30 min there after for material analysis; 5 After centrifuge in low temp the supernatant of the blood samples were collected and went through axon and anon exchange column treatment; 6 Treated blood sample was used for 13C labeled leucine, glucose lactic acid examination through mass spectrograph; 7 The exhale gas was collected for 13CO2 exam through gas-phase mass spectrograph; 8 CO2 total production rate (V13CO2), body various substances production, oxidation speed, and substances metabolic percentage all can be calculated through equations provided by references below; 9 Data was processed through student t test.

分亮氨酸、葡萄糖和乳酸三组各取健康新西兰兔16只，每组再分为高代谢脓毒症组和对照组两部分，建模方法同第一部分；2）三组分别先静脉弹丸式推注再持续灌注13C标记的亮氨酸、葡萄糖和乳酸；3）灌注前抽取动脉血作为背景值，灌注达150，160，170和180min分别抽取动脉血2ml用于质谱分析；4）实验开始后前30min中每5min收集呼出气一次，随后每30min收集呼出气一次，用于气相质谱分析；5）血样经过低温离心取上清液，过阳离子及阴离子交换树脂，再经衍生化处理；6）处理过的血样进入气相色谱－质谱仪，测量其13C标记的亮氨酸、葡萄糖和乳酸丰度；7）实验兔的呼出气通过13CO2气相质谱分析仪测定其中的13CO2丰度（E13CO2）；8）利用文献提供的公式算出CO2总生成率（V13CO2）以及机体各物质的通量、氧化速率以及物质代谢百分比等；9）数据分析处理采用t检验。

On the basis of plan form and crosscutting relationship of the faults, two kinds of tectonic systems were developed in Wu-Xia area, one was strike north-east, and another was strike east-west, the first was developed early and control the structural framework of Wu-Xia area, after that three times longitudinal compress action was happed. According to the intensity of tectonic deformation, The Wu-xia faulted zone from north to south can be divided into the thrust faulted belt, the fault fold belt and the monocline belt. from east to west have three regulatory faults, Wuerhe fault fold belt, Wuxia thrust faulted belt and Xiahong fault fold belt. Base on that 9 secondary structural unit can be divided.Structural accommodation zone and the distribution of reservoir strata have intimate business connection. Usually, Structural accommodation zone was the source zone of arenaceous sediment. The Wu-xia faulted zone have 3 kinds of structural accommodation, concordant approach, concordant overlap and concordant colinearity.

首先研究本区的构造特征，根据断裂的平面形态及相互切割关系，乌夏地区发育两种构造体系：北东向构造体系和近东西向构造体系，前者形成早，控制了该区的构造格局，晚期有3次近南北向挤压作用叠加；乌夏地区南北根据构造变形的强度不同分为冲断带、断褶带、单斜带，东西由于横向调节断层的存在分为乌尔禾断褶区、乌-夏冲断区和夏-红断褶区，进一步划分为九个次级单元；构造调节带与储集层的分布关系密切，通常是大量富砂质沉积的源区，乌夏地区发育三种类型的构造调节带：同向接近型、同向叠覆型和同向共线型。

Liaoning Medical University, Jinzhou 121001 China）Abstract:Objective To study the distribution and liver targeting of glycyrrhetinic acid derivatives (stearin glycyrrhetinic acid ester-3-O-galactosidase, Gal-GAOSt) modifying norcantharidin liposomes (Gal-GAOStNC-LP) in rats.

目的 研究甘草次酸衍生物（甘草次酸硬脂醇酯-3-O-半乳糖苷，Gal-GAOSt）修饰去甲斑蝥素脂质体(Gal-GAOStNC-LP)在小鼠体内的分布及肝靶向性。方法利用薄膜分散法制备甘草次酸衍生物修饰去甲斑蝥素脂质体后，经小鼠尾静脉给药，采用HPLC法检测去甲斑

The median patient age was 41 years, 1 female and 3 male. The patients received BMSCs infusion at a dose of (1.0-2.0)×107 cells every time by intrabone marrow injection from the anterosuperior iliac spine and BMSCs from the same donor for the same patient were infused more than once.

骨髓间充质干细胞髓内注射具体方法：取右髂前上棘为穿刺注射点，注射骨髓间充质干细胞悬液，4例患者的给药剂量基本控制在(1.0~2.0)×107个细胞/次，行间断多次给药。

In the normal uterus, Cytokeratins immunolabelling were detected in glandular cell, luminal epithelial cell, Vimentin immunolabelling were detected in stromal cell and endoblastic cell; CK7 immunolabelling were not detected in any tissue of the yak utenus.

研究结果显示：未妊娠时，泛角蛋白在子宫内膜腺上皮细胞、腔上皮细胞内表达，波形蛋白在子宫内膜基质细胞内表达，平滑肌肌动蛋白在子宫平滑肌和血管平滑肌内表达，牦牛子宫任何部位均不表达角蛋白7；妊娠30天左右时，泛角蛋白在子宫内膜腺上皮细胞、子宫内膜腔上皮细胞、滋养层细胞、内胚层细胞和尿囊细胞内表达，波形蛋白在子宫内膜基质细胞和内胚层细胞内表达，平滑肌肌动蛋白在子宫平滑肌和血管平滑肌内表达，角蛋白7在尿囊细胞内表达，偶尔在腔上皮细胞的细胞核边缘表达；消化法进行原代培养时，组织经胶原酶消化并通过100目和400目筛网组合可以有效地分离原代子宫内膜基质细胞和子宫内膜腺上皮细胞；分离得到的子宫内膜基质细胞活率达90%以上，并可在体外传代7次以上；分离得到的子宫内膜腺上皮细胞活率可达85%以上，并可在体外传代5次以上；RPMI1640培养基最适合子宫内膜基质细胞和子宫内膜腺上皮细胞的生长，维持子宫内膜基质细胞正常生长的FBS添加量为20%，维持子宫内膜腺上皮细胞正常生长的胎牛血清添加量为30%。

Our study show that 9, 11-Octadecadienoic acid, methyl ester is the highest content of organic acid ester components in non-hydrocarbon fraction, higher content of 6-Octadecenoic acid, methyl ester reflects mainly terristrial source input, higher content of pentadecane acid, 14-methyl, methyl ester or Heptadeeanoic acid, 16-methyl, methyl ester shows mainly aquatic source input.

原油和岩石抽提物非烃中有机酸酯类化合物的有机地球化学研究报道很少，系统研究发现非烃有机酸酯中9，11-十八二烯酸甲酯含量最高，而次高峰的丰度可用作生源判识，陆源母质输入多的样品次高峰为6-十八单烯酸甲酯，水生母质供给多者次高峰为14-甲基十五烷酸甲酯或16-甲基十七烷酸甲酯。

You BE Evolution incompletely of life body, gene mutation of Alien, The senior high school of kindergarten degree living, inborn Mongolia the frog head of disease, The abandoned baby of Mount Everest snow man, the septic tank stop of murderer, The Africa make top black*of descendant, the yin and yang out of tune chimpanzee, The hippo been ran over by the Noah's ark, new volcano eruption, Super big shameless transmit sound to expand a sound trumpet, the humiliation of the Eskimo, With cockroach coexistence live of super individual, the vitality putrefied half plant, Will send out bad smell of**person,"cast off" the source head of word, Everyday deteriorate three time dinosaur, mankind history top most strongly discard a material, God lose the old washer which fall off, ability thinking of have no head living creature, Damage the disaster of compatriot fame in Asia, the ancestry receive for it abashed of descendant, The Fu of deposition thousand years plant quality, the scientist also dare not a research of originality species, The deposition raw material of 1000% petroleum densities, is disfigure of McDonald's uncle, Be like your this kind of damned guy: Only one Tuo muck that can play the inside of the television drama, Canned not compare with a wayside the chewing gum be spread urine by the dog, Connect such as flower all handsome you 1000% above, Seek the girl friend get to even want to leave the Earth to the zoo, Wanting to commit suicide would someone advise you and don't leave corpse in order to prevent a pollution environment, You once touched of keyboard top connect rice the protozoa all live not bottom go to, Jet come of the saliva return than the SARS fatal, Pack the lovely words can the moment solve population inflation of problem, Play cool pack handsome words mankind to have to use asexual reproduction, The idiocy can be you of teacher, mentally retarded can teach you how to say person's words, Want ～only your rise ozone layer meeting hole Wanting to immigrate Mars is in order to leave you, If you of ugly can generate electricity of the nuclear power station of words whole world can be with the lockout, The words bullet missile fight would be unbearable to fly toward you, The hand grenade see you would from explode, Other people would not° until open an airplane to bump a pair of son star line but you as long as bail out and then have same of power, All of the famous spot that you have been to change historic monument, you have been to of the historic monument will become history, 18 generation son all have no stem good matter would understanding you, connect throw into the sun all suspicion not enough environmental protection Anyway horizontal Shu a words:Don't let me see you again, if saw you, I must put out you!

你是 进化不完全的生命体，基因突变的外星人，幼稚园程度的高中生，先天蒙古症的青蛙头，圣母峰雪人的弃婴，化粪池堵塞的凶手，非洲人搞上黑*的后裔，阴阳失调的黑猩猩，被诺亚方舟压过的河马，新火山喷发口，超大无耻传声扩音喇叭，爱斯基摩人的耻辱，和蟑螂共存活的超个体，生命力腐烂的半植物，会发出臭味的**人，&唾弃&名词的源头，每天退化三次的恐龙，人类历史上最强的废材，上帝失手摔下来的旧洗衣机，能思考的无脑袋生物，损毁亚洲同胞名声的祸害，祖先为之蒙羞的子孙，沉积千年的腐植质，科学家也不敢研究的原始物种， 10倍石油浓度的沉积原料，被毁容的麦当劳叔叔，像你这种可恶的家伙：只能演电视剧里的一陀粪，比不上路边被狗洒过尿的口香糖，连如花都帅你10倍以上，找女朋友得去动物园甚至要离开地球，想要自杀只会有人劝你不要留下尸体以免污染环境，你摸过的键盘上连阿米吧原虫都活不下去，喷出来的口水比SARS还致命，装可爱的话可以瞬间解决人口膨胀的问题，耍酷装帅的话人类就只得用无性生殖，白痴可以当你的老师，智障都可以教你说人话，只要你抬头臭氧层就会破洞要移民火星是为了要离开你，如果你的丑陋可以发电的话全世界的核电厂都可以停摆，去打仗的话子弹飞弹会忍不住向你飞，手榴弹看到你会自爆，别人要开飞机去撞双子星才行而你只要跳伞就有同样的威力，你去过的名胜全部变古迹，你去过的古迹会变成历史， 18辈子都没干好事才会认识你，连丢进太阳都嫌不够环保反正横竖一句话：别让我再看见你，要是见着了你，我一定要把你灭了！

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length P1, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length P1, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3. 1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus , which were expressed in BHK-21 cells, were confirmed by sandwich-ELISA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3. 1/IFN which includes the gene IFN-α of cattle. Subsequently, Recombinant plasmids were injected to cattles with or without pcDNA3. 1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies titers were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫苗，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接/酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1/P12X3C和pcDNA3.1/P12X3C3D、pTARGET/P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1/IFN分别/同时免疫，第二次免疫后第三周豚鼠攻以1OOID〓或1000ID〓的O型FMDV China99株；随后将质粒pcDNA3.1/P12X3C、pcDNA3.1/P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1/IFN同时免疫牛，三周后经牛舌皮攻以10〓ID〓的O型FMDV China99株。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length PI, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length PI, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3.1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus, which were expressed in BHK-21 cells, were confirmed by sandwich-ELlSA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P 12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3.1/lFN which includes the gene IFN-a of cattle. Subsequently, Recombinant plasmids were injected to catties with or without pcDNA3.1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies liters were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫曲，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接／酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1／P12X3C和pcDNA3.1／P12X3C3D、pTARGET／P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1／IFN分别／同时免疫，第二次免疫后第三周豚鼠攻以100ID_(50)或1000ID_(50)的O型FMDV China99株：随后将质粒pcDNA3.1／P12X3C、pcDNA3.1／P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1／IFN同时免疫牛，三周后经牛舌皮攻以10~4ID_(50)的O型FMDV China99株。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。