负鼠

In addition the chance of a mammoth home, and her own, however, as the opossum and opossum, and two brothers live together, they joined the team, and with the run.

而巧遇另外一只母猛犸象，而她却将自己当作负鼠，并且和两个负鼠兄弟生活在一起，他们加入了这个团队，并一起逃亡。

Omnivorous opossum of the eastern United States; noted for feigning death when in danger; esteemed as food in some areas; considered same species as the crab-eating opossum of South America.

美国东部的杂食性负鼠；以危险时装死而闻名；在有些地区被当作珍贵的食物；南美洲的一些品种被当作食蟹负鼠。

We show that these germline infiltrations likely occurred around the same evolutionary time (15–46 mya) and spawned some of the largest bursts of DNA transposon activity ever recorded in any species lineage (nearly 100,000SPINcopies per haploid genome in tenrec).

这组科学家在调查夜间活动的灵长类动物——丛猴的DNA转座子的时候发现了这些成分，在经过进一步检查之后，他们在其他物种中也发现了这些"侵略者"，包括大鼠和小鼠、负鼠和非洲爪蛙。

The RT-PCR product was inserted into pTG19-T vector and transformed into E. coli successfully. By blastn, the sequence results of Kunming mus musculus were in complete accordance with the conservative sequence of Genbank NR_003278 (791bp-1153bp). By Blastn in NCBI, the sequence with little difference among animals was confirmed to be conservative. After Blastn, fourteen complete CDS coding for different animals were chosen. According to VECTOR NIT 9.0 software, the similarities between Kunming mus musculus and bos taurus, homo sapiens, erinaceus europaeus, cricetulus griseus, sus scrofa, dasypus novemcinctus, rattus norvegicus, rabbit, equus caballus, macaca fascicularis, didelphis virginiana, monodelphis domestica and vombatus ursinus was 67%, 100%, 100%, 36%, 100%, 100%, 67%, 100%, 100%, 92%, 99%, 99% and 99%. In the phylogenetic tress constructed with the forteen 18S rRNA by Treeview, the Kunming mus musculus clustered with cricetulus griseus, sus scrofa and rabbit, which was nearer to cricetulus griseus and was most far away from macaca fascicularis.(3) After sencodary structure analyses of 18S rRNA of mus musculus, an oligonucleotide fragment for RNAi was designed and synthesized, which was transformed into plasmid, and restriction enzyme analyses and sequencing results should the expression plasmid pGPH1/ GFP/Neo-mouse-sh 18S rRNA were constructed for RNAi successfully.

结果①通过RT-PCR检测显示18S rRNA基因在小鼠卵巢组织和单个GV期、MⅠ期卵母细胞中均有表达，且在未成熟卵母细胞中，MⅠ期的表达明显强于GV期的表达；②RT-PCR产物克隆测序结果显示：昆明小鼠18S rRNA基因保守区序列与基因库序列[NR_003278保守区部分(791bp～1153bp)]完全一致；Blastn比对结果发现：在不同物种中差异较小，选出14种生物18S rRNA全序列经VECTOR NIT 9.0软件分析，提示昆明小鼠18SrRNA与牛、人类、刺猬、中国仓鼠、猪、犰狳、褐鼠、兔子、马、食蟹猴、负鼠、短尾猊、袋熊的18S rRNA的相似率依次为67%，100%，100%，36%，100%，100%，67%，100%，100%，92%，99%，99%，99%；Clustal 1.81和Treeview构建出的分子进化树表明：在上述14种生物中昆明小鼠与中国仓鼠进化关系最近，与兔子、猪聚成一簇，与食蟹猴进化关系最远；③根据18S rRNA二级结构设计并合成RNA干扰寡核苷酸片段，重组质粒经过限制性内切酶及测序表明成功构建了pGPH1/GFP/Neo-mouse-sh 18S rRNA干扰表达质粒。

Any of various nocturnal, usually arboreal marsupials of the family Didelphidae, especially Didelphis marsupialis of the Western Hemisphere, having a thick coat of hair, a long snout, and a long prehensile tail.

负鼠一种夜间行动的杂食性树栖种袋鼠，尤指生长于西半球的负鼠，毛皮粗糙厚实，身体较长且粗，长有缠绕性的长尾

Actually also very much has a headache, beautiful Li is adopted since childhood by the didelphis lanigera family, has been contaminated all didelphis lanigera's habit, crawls the tree, drills the corner and so on.

其实Manny也很头疼，美丽的Ellie从小被负鼠家族收养，浸染了所有负鼠的习性，爬树，钻墙角等。

Get up close to wallabies, possums, echidnas and potoroos on one of the many bushwalks.

在众多丛林健行路线上近距离观察岩袋鼠、负鼠、针鼹鼠以及长鼻袋鼠。

Possums were possums and mammoths were mammoths.

负鼠是负鼠、长毛象是长毛象。

Possums were possums and...

负鼠是负鼠。

The most common possum species in Australia, the brushtail possum is a tree-dwelling nocturnal marsupial.

在澳大利亚，刷尾负鼠是最常见一种负鼠。它们是一种居住在树上并且喜欢夜间活动的有袋动物。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。