豚鼠

Methods By using standard intracellular microelectrode technique to record action potentials, various doses of Ligs were used to investigate their electrophysiological effects on SP-induced effects of guinea pig papillary muscles.

利用常规玻璃微电极细胞内记录技术，观察SP对豚鼠心室乳头肌动作电位的影响及不同剂量Lig的干预作用。

Methods: An animal model of pigment gallstones was established in male guineapigs by hypodermic injection of lincomycin.

1.3 动物分组、造模及药物处理实验豚鼠随机分为空白组、模型组、清胆胶囊组和养肝利胆颗粒组，每组各8只。

Methods Guinea pigs were used to prepare models of asthma,and then the level of products of lpometabolism-MDA and LYM and their correlation at different time points(1 hour and 24 hour) were explored in the attack stage and after intervention.

制作豚鼠哮喘模型，检测哮喘发作及激素干预后不同时间点(1、2 4h)LPO代谢产物丙二醛、血淋巴细胞水平及两者的相关性。

Lidocaine and cefazolin sodium solution 1ml each were added to 20ml of HPAG. Then the mixed gel were injected into subdermis or mascle of guinea-pigs 0.5～2ml at each point with 18 gauge needle.

实验组以2%利多卡因1ml与头孢唑啉钠溶液1ml稀释水凝胶后，用18号注射针头将其注入到豚鼠皮下或肌肉层，每个部位注射量为0.5～2ml。

The technique of liver CYP450 enzymes was applied to the in vitro study of drug metabolism In the first part of this article, the metabolism of nimodipine in human liver microsomes was studied and in the second of part of this article the metabolism of clivorine in female rat, guinea pig, human liver microsomes as well as the mechanism of metabolism-induced toxicity were investigated.

运用肝微粒体技术进行药物的体外代谢研究。本论文第一部分运用肝微粒体技术研究了尼莫地平在人肝微粒体内的代谢及其代谢的酶动力学，第二部分研究了肝毒性生物碱Clivorine在大鼠、豚鼠和人肝微粒体内的代谢及其代谢致毒机理。

Immunofluorescence was used to observe the protein expressions of the 5 muscarinic receptor subtypes.

结果豚鼠巩膜组织中有M受体五种亚型mRNA的表达，其中以M1亚型的表达最高。

It is suggested that the HAR may be the main cause of the impaired reperfusion of the xenograft microcirculation.

结果表明豚鼠至大鼠肝移植后发生的超急性排斥反应是造成移植肝微循环再灌注障碍的主要因素。

METHODS: Lipofectamine method: The BMMSCs were obtained from the tibias and femurs of the guinea pigs. The third passage BMMSCs were cultured with plasmid-lipofectamine mixture for 6 hours, followed by fetal bovine medium for 48 hours. Immunohistochemistry was performed for transient expression. G418 was added after 48 hours.

①脂质体法：取体外分离、培养的第3代豚鼠骨髓间充质干细胞，将质粒-脂质体混合物加入含细胞的培养基中培养6 h，再加入胎牛血清的培养基，孵育48 h 后行免疫组织化学检测，即为瞬时表达。48 h后加入含G418培养基筛选。

Methods After feeding a high-cholesterol diet to guinea pigs, Oddi′s sphincters were disseced on day 30 and day 60 respectively. We used RT-PCR, western-blotting to evaluate expression level of calponin gene.

饲予豚鼠高胆固醇饮食，分别在30天和60天取出Oddi括约肌，应用逆转录聚合酶链反应和蛋白质免疫印迹法检测动物Oddi括约肌调宁蛋白基因的表达水平。

Objective To establish HPLC-UV method for determination of glycyrrhizin acid in compound nasal spray of glycyrrhizin and flower flos magnoliae,the chief active ingredient of formulation,glycyrrhizin acid,and to observethe therapeutic effect of this formulation on guinea pig's hypersensitive rhinitis.Methods A compound nasal spray formulation was prepared using extracts of glycyrrhizin and measured by HPLC-UV method.Mobile phase conˉsisted of methanol,0.2mol/L ammonium acetate and glacial acetic acid（67:33:1,v/v）,analytical column was Zorbax ODS C 18 and wavelength250nm.

目的 建立测定复方甘草和辛夷喷雾剂含量的方法并观察该制剂对豚鼠超敏性鼻炎的疗效方法以甘草提取的甘草甜素和辛夷花蒸馏提取液主药制备成鼻用喷雾剂，用反相高效液相色谱法测定制剂中甘草酸含量，检测波长为250nm，Zorbax ODS C 18 分析柱，流动相为甲醇：0.2mol/L醋酸铵：冰醋酸（67：33：1）。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。