豆蛋白酶

In order to optimize enzymolysis technology for production of antioxidant peptides from chickpea protein, effect of ratio of enzyme to substrate, enzymolysis temperature, and hydrolysis time on the technology in which reducibility and superoxide anion radical capturing rate were taken as response values was analyzed with response surface methodology.

为优化Alcalase蛋白酶酶解鹰嘴豆蛋白制备抗氧化肽的工艺条件，采用响应面分析法，以还原能力、超氧阴离子捕获率为响应值，研究了酶与底物的比值、酶解温度和酶解时间对制备抗氧化肽工艺的影响。

The differences of hydrolysis degree, antioxidative activity, moisture absorption, moisture retention and oil absorption between soybean peptides and chickpea peptides were mainly investigated and the influences of the protease kinds on the functional properties of peptides were also considered.

研究付比了鹰嘴豆肽和大豆肽的水解度、抗氧化性、吸油性、吸湿及保湿性等功能特性的差异，以及蛋白酶种类对肽产物功能性质的影响。

Ratio of enzyme to substrate, temperature, and hydrolysis time for the production of antioxidant peptides were analyzed with Response surface methodology in which reducibility and superoxide anion radical capturing rate was response value to build a predictive model for the enzymolysis of chickpea protein and the optimization of the enzymolysis technology parameters.

为优化Alcalase蛋白酶酶解鹰嘴豆蛋白制备抗氧化肽的工艺条件，采用响应面分析法（response surface methodology，简称RSM），以还原能力、超氧阴离子捕获率为响应值，研究了酶与底物的比值、酶解温度和酶解时间对制备抗氧化肽工艺的影响。

The purpose is to study the enzyme characterizations of neutral protease fermented by synergism strains of Bacillus natto SH and B1. The optimal reaction temperature, optimal reaction pH, pH stability, thermal stability, metal ions, surface-active agent, inhibiter, simulated intestinal environment on enzyme stability were determined by Folin-phenol method, diversify graphs of residual activity were drawed.

为研究纳豆芽孢杆菌SH和B1配伍发酵产物中中性蛋白酶的性质，通过Folin-酚法测定中性蛋白酶的最适作用温度、最适作用pH值、pH稳定性、热稳定性和金属离子、表面活性剂、抑制剂、模拟胃肠道环境对酶活力的影响，绘制相对酶活力变化图。

To produce the scientific evidence for developing and manufacturing new antitumor drugs.Methods: 1 The inhibitory effect on cell growth of Hela was measured by MTT assay in treated or untreated groups (3.125, 6.25, 12.5, 25, 50μg/ml TAM and control) for three different treatment times (24h, 48h and 72h).2 Apoptosis and cell cycle were measured by FCM in four experimental groups (0, 4, 16, 40μg/ml TAM) for 48h.3 Adopting Wright and Giemse's staining to observe the morphology of Hela cells which treated with 40μg/ml TAM.4 Using invasion experiment to detect the Hela cells'invasive abilities which treated with 40μg/ml TAM.5 The protein expressional levels of P-ERK, ERK, C-myc and Cyclin D1 in Hela cells untreated or treated with 4, 16, 40μg/ml TAM for 24h were measured by Western blot.6 Expression of anti-apoptotic gene bcl-2, apoptotic gene bax and MMP-9 in Hela cells of four experimental groups (0, 4, 16, 40μg/ml TAM for 24h), were observed by revers transcription PCR.7 The protein expression of P-ERK, ERK, Bcl-2 and Bax in Hela cells treated with 40μg/ml TAM for 24h observed by laser scanning microscopes.

1采用四甲基偶氮唑蓝法检测不同浓度北豆根总碱(3.125、6.25、12.5、25、50μg/ml)处理不同时间(24、48和72小时)对Hela细胞增殖反应的抑制作用。2采用流式细胞技术(flow cytometry，FCM)检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用48小时，对Hela细胞凋亡及周期变化的影响。3瑞氏-姬姆萨染色后显微镜观察北豆根总碱(0、40μg/ml)作用24小时后Hela细胞形态学变化。4采用Transwell小室法检测北豆根总碱(0、40μg/ml)作用24小时后对Hela细胞侵袭性的影响。5采用免疫印迹方法检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用24小时后，Hela细胞中磷酸化ERK、ERK、C-myc、CyclinD1的表达变化。6采用逆转录-聚合酶链反应(revers transcription PCR，RT-PCR)半定量检测北豆根总碱(0、4、16、40μg/ml)作用24小时，Hela细胞抗凋亡基因bcl-2、促凋亡基因bax、基质金属蛋白酶-9(MMP-9)的表达变化。7应用激光共聚焦显微镜(laser scanning microscope，LSM)观察北豆根总碱(0、40μg/ml)作用24小时后，Hela细胞内P-ERK、ERK、Bcl-2、Bax蛋白的表达变化。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。