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The profiles of pathogenic strains had less difference from each other but more difference from nonpathogenic strains.

致病性钩端螺旋体间MRSP谱型差别相对较小,遗传距离较近,而它们与非致病性钩端螺旋体的MRSP谱型相差大,遗传距离远。

Membrane affinity quantified by IAM and liposome/buffer systems was a more efficient predicator of α values than hydrophobicity from n-octanol/buffer system. Consequently, not only hydrophobic force but also attractive polar extra-interactions involved in the binding to IAM and liposomal membranes but not in an n-octanol phase, ascribed to the binding process within AM. The binding to ordered lipid membrane in the larger part determined drugs'binding to AM intracellular compositions, and the ordered phospholipid plasma membrane was a potential site for drugs'binding in AM.

脂质体/水系统和磷脂膜色谱所测定的膜亲和性比正辛醇/水系统的疏水性更好的预测巨噬细胞内药物的α值,因此包含在磷脂膜色谱中但并不体现在正辛醇/水系统中药物与磷脂膜之间的额外吸引性极性作用力对药物在细胞内结合有着重要贡献;药物与有序磷脂膜的结合在很大程度上决定着药物与巨噬细胞内成分的结合,有序磷脂分子膜可能是巨噬细胞内部潜在的结合部位。

Objective To develop a RP-HPLC method for the enantiomeric separation of indapamide on a new chiral column CHIRALCEL OD-RHcellulose tris(3,5-dimethylphenylcarbamatecoated on a5μm silica-gel substratefor pharmaceutical study of indapamide.Methods To obtain optimistic method for the enantiomeric separation of indapamide,the inferences of the composition of mobile phase,the flow rate and the temperature on the enantiomer retentivity and selectivity were examined and discussed.

目的采 用新型反相手性色谱柱CHIRALCEL OD-RH(纤维素3,5-二甲基苯基氨基甲酸酯涂敷在5μm硅胶上),建立吲达帕胺对映体的反相高效液相色谱拆分方法,为研究吲达帕胺手性对映体药代动力学提供基础。

In this paper, total logistics amounts are chosen as evaluation index, and artificial time series are designed to solve the shortness of statistical data in Chinese logistics. Spectrum estimation theory depicts time characteristics in frequency domain thus has such benefit than time domain methods. This paper evaluates the period of Chinese logistics using spectrum estimation. Stationarity of artificial series is verified, parameter sensitivity of truncation point is analyzed, six periods with different delay window are compared, and significance of period evaluated is tested.

中国物流统计数据缺乏,客观上限制了对其周期规律的研究,本文选取社会物流总额作为测度指标,设计了一种人工序列,解决了统计数据过短问题;谱估计法从频域角度揭示客观事物的时间特性,在研究时间序列周期波动方面具有时域分析法所不具备的优势,作者采用谱估计法对中国物流发展周期进行测定,检验了指标序列平稳性,分析了截断点参数灵敏度,比较了6种不同滞后窗下周期值,并检验了所测得周期的显著性。

ABSTRACT: Random amplified polymorphic DNA was used in analysing the relationship among twenty-six speices of Amanita from Mangshan, Hunan. Of 40 primers, 6 ones generated reproducible RAPD profiles, and each one produced 1-10 bands. Two primers (OPG15 and OPH04) could differentiate 26 species of Amanita by its RAPD profiles. The similarity coefficients obtained from profiles generated by six primers among Amanita were about 20-60%. A dendrogram was constructed using average linkage clustering for clustering analysis. It indicated that there were two groups in 26 species and some species with ring and membranous volva clustered a group which coincided with morphological classification.

本文对采自湖南莽山的26种鹅膏菌属真菌进行了随机扩增多态性DNA分析,40个随机引物中筛选出扩增效果较好的6个引物,每个引物能产生1~10条DNA条带,获得的RAPD谱带清晰并呈现多态性OPG15、OPH04两个引物扩增的RAPD谱带能将26种鹅膏菌完全区分开来,通过6个引物的RAPD分析获得的平均相似性系数表明种与种之间的相关系数在20―60%之间,平均链锁聚类分析可将26种鹅膏菌分为二大类,且一些具菌环和苞状菌托的种类聚在一起,与形态分类基本相吻合。

The content ofone liver protein band (R_f=0.79) at 1℃was significantly higher than at room temperature (P<0.05).On the 2-D native-PAGE/SDS-PAGE gel, the content of P spot (MW=51kDa, R_1=0.47on the 1D native PAGE gel) at 1℃was significantly higher than at room temperature (P<0.05), and the content of N spot (MW=65 kDa, R_f=0,94 on the 1D native PAGE gel) at roomtemperature was significantly higher than at 1℃. These protein spots may be related with the adaptation to the low temperature of the Heilongjiang wood frog.

黑龙江林蛙肝脏蛋白电泳显示,胶浓度6%的肝脏PAGE图谱分辨率高、谱带多,可分辨出15~19条谱带,其中1℃组R_f=0.79蛋白含量比室温组显著增加(P<0.05)。2-D native-PAGE/SDS-PAGE电泳时,胶浓度为12%时分离效果较好,蛋白斑点较多。1℃组中P蛋白斑点(MW=51kDa,一向PAGE的R_f=0.47)显著性上调(P<0.05),N蛋白斑点(MW=65kDa,一向PAGE的R_f=0.94)显著性下调(P<0.05),推测这两种蛋白可能与黑龙江林蛙在低温环境下的适应性变化有关。

The method examines by national import and export merchandise occupation standard SN0352-95:" Exit shellfish is paralytic " of law of proved recipe of check of sexual shellfish toxin determines the; of content of toxin of paralytic sex shellfish of snail flesh and its content content applies fluid photograph color atlas, mass spectra couplet is used detect toxin of snail flesh balloonfish and its ramification.

方法按国家进出口商品检验行业标准SN0352-95:&出口贝类麻痹性贝类毒素检验方法&测定螺肉及其内容物的麻痹性贝类毒素含量;应用液相色谱—质谱联用检测螺肉河豚毒素及其衍生物。

Studies on genetic diversity: 252 bands were amplified by 20 primers in Carya cathayensis ,168 bands were polymorphic and PPB was 66.7%. 238 bands were amplified by 20 primers in C.dabieshanensis .162 bands were polymorphic and PPB was 68.1%.

群体遗传多样性研究:20条引物在山核桃群体中共扩增出252条谱带,其中,多态性条带168条,PPB为66.7%;20条引物在大别山山核桃群体中共扩增出238条谱带,其中,多态性条带162条,PPB达68.1%。

ConclusionThe method is reliable, stable and repeatable and can be used for the analysis of the volatile constitiuents of Evodia lepta leaves.

结论此方法稳定可靠,重现性好,可用于三叉苦叶挥发性成分的分析。三叉苦叶;挥发油;气相色谱-质谱联用

Insection 4.3, semi-discrete and fully discrete Fourier spectral and pseudo-spectral schemes are constructed. The convergence and stability for the schemes are proved, and the error estimates are obtained.Chapter 5, consider the damped coupled generalized nonlinear wave equations. In section 5.2, by coupled a priori estimates and Galerkin method, prove the existence and uniqueness of the global smooth solution for the periodic initial value problem and obtain the existence of global attractors.

第四章,考虑一类具耗散的广义KdV方程组的周期初值问题,在第二节中证明了整体光滑解的存在性和唯一性,得到整体吸引子;在第三节中构造了半离散和全离散的Fourier谱格式和拟谱格式,在整体光滑解存在的条件下,证明了这些格式解的收敛性,并得到了误差估计。

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In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国,慢性酒精中毒,肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题,你可以随时联系我。

Very pretty, but the airport looks more fascinating The other party wisecracked.

很漂亮,不过停机坪更迷人。那人俏皮地答道。