谱性

Proteins associated with the membrane and the cytosolic portions of the proteome under different copper ion concentrations were differentially labeled with an acid cleavable isotope-coded affinity tag reagent, and the labeled peptides were separated by two-dimensional chromatography and characterized by electrospray ionization-tandem mass spectrometry.

我们分别将在不同铜离子浓度下的细胞质以及细胞内膜中所有蛋白质标识上酸裂性同位素亲和性标识(acid cleavable isotope-coded affinity tag， cICAT)试剂，用二次元管柱层析将所有标识并酵素水解过的片段分离，并且用电洒式串连质谱(electrospray ionization-tandem mass spectrometry， ESI-MS/MS)以及基因体定序资料库决定蛋白质序列。

Based on these previous work, in this research, in order to explore the the molecular mechanism of enhanced tumor cell immunogenicity treated by elemene and mytomycin C compositely, we used murine Hca-F ascitic hepatic carcinoma ( high lymphatic metastasis substrain of H22) as an experimental model to compare the immunoprotective effects on Hca-F cells as well as the mechanism of the effects caused by HSP70-peptide complex purified from untreated tumor cells, EC-TCV and BCG; We studied the mechanism of increased tumor cell membrane HSP expression treated with elemene by the techniques of RT-PCR and immunoelectron microscopy; In order to deeply elucidate the antitumor effects and the molecular mechanism of elemene in increasing tumor cell immunogenicity we analyzed and compared the effects of elemene and heat shock on the gene expressions of tumor cell using gene expression profile chip.

本论文在上述研究成果的基础上，选择小鼠肝癌H22的高淋巴道转移亚系Hca-F为模型，进一步比较了未处理肿瘤细胞来源、榄香烯复合瘤苗来源的和卡介苗来源的HSP70-肽复合物对Hca-F的免疫保护效应、效应机制，探讨榄香烯-丝裂霉素复合处理增强肿瘤细胞免疫原性的分子机制；以Hca-F和人肝癌细胞株HepG〓等为模型研究了榄香烯处理增强肿瘤细胞膜热休克蛋白表达的机制；以HepG〓为模型用表达谱基因芯片研究比较了榄香烯与热休克处理对肿瘤细胞基因表达的影响，从而进一步阐明榄香烯抗肿瘤作用及增强肿瘤细胞免疫原性的分子机制。

The fingerprints of 10 species including 27 accessions in genus Corchorus were investigated with the technique of RAPD.Twenty-five primers were screened from 119 random primers,and a total of 329 DNA fragments were amplified ranging from 0.3～3.0 kb,253 (87.78%),which were polymorphic.

采用RAPD标记构建了黄麻属植物10个种27份材料的指纹图谱，从119个随机引物中筛选出清晰且多态性高的25个引物，共扩增出329条DNA片段，分子量在0.3～3.0 kb之间，其中294条谱带具有遗传多态性，多态比率为89.36％，平均每个引物扩增出13.16条带。

The fingerprints of 10 species including 27 accessions in genus Corchorus were investigated with the technique of RAPD. Twenty-five primers were screened from 119 random primers, and a total of 329 DNA fragments were mplified ranging from 0.3~3.0 kb, 253 (87.78%), which were polymorphic.

采用RAPD标记构建了黄麻属植物10个种27份材料的指纹图谱，从119个随机引物中筛选出清晰且多态性高的25个引物，共扩增出329条DNA片段，分子量在0.3～3.0 kb之间，其中294条谱带具有遗传多态性，多态比率为89.36％，平均每个引物扩增出13.16条带。

Based on the higher alcohols and lactic acid metabolism theory, a method was developed to low higher alcohols capability Saccharomyces cerevisiae strains by screening high lactic acid dehydrogenization enzyme activity in this study.

本研究对Y1110菌株及其出发菌株、突变等株16株不同来源的酵母菌株进行全基因组RAPD分析，建立并优化了酵母RAPD扩增体系和分型系统，从20条随机引物中筛选出了3条具有较好的扩增多态性，共扩增出34条RAPD谱带，多态性为85.3％；获得了稳定清晰的RAPD指纹图谱。

Fingerprints of broiler chickens divergently selected for very low den sity lipoprotein in fat line and lean line of the third generation were studied using inter simple sequence repeated technique. Geneti c polymorphism and genetic similarity coefficient between two groups were analyze d. Ten primers were screened from 60 SSR primers and 62 sites were deprived. Of which 39 bands were polymorphic sites and the frequency was 63 percent.

通过对初步进行血浆极低密度脂蛋白双向选择的三世代高、低脂群肉鸡进行ISSR指纹分析，评定其遗传多态性及两群体间的遗传相似系数。60个引物筛选出10个引物用于检测分析；共扩增出62条谱带，其中39个是多态性片段，多态率为63%。

Methods 209 patients with CHD and 101 controls were selected. MTHFR genetic C677T polymorphism was determined by PCR-RFLP, and CBS T833C polymorphism by ARMS method. Plasma homocysteine levels were detected with HPLC. Results The frequencies of MTHFR T homogenetic type and heterogenetic type (27.8%and 45.4%) in case group were higher than those in normal group(22.8%and 34.6%).

对209例冠心病患者和101例健康对照者，采用多聚酶链反应-限制性内切酶片段长度多态性技术检测MTHFRC677T基因型，用扩增阻滞突变体系法检测CBS基因T833C多态性，采用高压液相色谱法测定同型半胱氨酸血浆水平。

Libellula had different resistance to different races and Xiannong 4 was susceptible to all the races except CY18 and CY22. Resistant components had significant variances among different cultivars, races and cultivar×race. And Libellula had strong selection on different races, while the selection of Xiannong 4 was weaker than that of Libellula.

里勃留拉的抗性谱较宽，抗病性在小种间差异明显，而咸农4号对除CY18、CY22外的小种均感病；两者的抗病性组分在品种间、小种间以及品种×小种互作时均有显著差异；里勃留拉对不同菌系有较强的选择作用，而咸农4号的选择作用较弱。

Based on relative literatures,this part has included five resesearch aspects as below.1 Fifteen compounds were isolated and purified by extraction,column chromatography,and their structures were determined on the basis of spectral analysis: acteoside(Ⅰ-1),isoacteoside(Ⅰ-2),crenatoside(Ⅰ-3),cistanoside F(Ⅰ-4),sinapoyl-4-O-β-D-glucoside(Ⅰ-5),adenosine(Ⅰ-6),β-siterol(Ⅰ-7),oleanic acid(Ⅰ-8), succinic acid(Ⅰ-9),caffeic acid(Ⅰ-10),protocatechuic aldehyde(Ⅰ-11),p-hydro xybenzyl alcohol(Ⅰ-12),β-daucosterol(Ⅰ-13),D-galacitol(Ⅰ-14),D-mannitol(Ⅰ-15).Ⅰ-4～15 were obtained from this plant for the first time,andⅠ-6,7,9,and 13 were isolated from Orobanche genus for the first time.2 The scavenging test of DPPH showed that most compounds have comparative antioxidant activity as L-ascorbic acid and part of them show better activity such as the O.coerulescens extract and phenylethanoid glycosides.Acteoside showed potent free radical scavenging effects with a median inhibition concentration of 25.6μg/ml.3 The anti-HBV activities of acteoside,isoacteoside and crenatoside were measured,and all of them showed suppressive activity on the expression of HBsAg and HBeAg in the HepG2.2.15 cell line.

本论文在文献调研基础上对紫花列当化学成分及生物活性进行了研究，并从免疫抗病毒角度探讨紫花列当中特征性成分类叶升麻苷的肝保护作用及其机制。1采用大孔树脂、硅胶和Sephadex LH-20等色谱技术对紫花列当进行系统的植物化学研究，从中分离得到19个化合物，利用UV和NMR等波谱手段及理化性质鉴定了其中的15个化合物，分别为类叶升麻苷(Ⅰ-1)、异类叶升麻苷(Ⅰ-2)、crenatoside(Ⅰ-3)、cistanoside F(Ⅰ-4)、sinapoyl-4-O-β-D-glucoside(Ⅰ-5)、腺苷(Ⅰ-6)、β-谷甾醇(Ⅰ-7)、齐墩果酸(Ⅰ-8)、琥珀酸(Ⅰ-9)、咖啡酸(Ⅰ-10)、原儿茶醛(Ⅰ-11)、对羟甲基苯甲酸(Ⅰ-12)、β-胡萝卜苷(Ⅰ-13)、D-半乳糖醇(Ⅰ-14)和甘露醇(Ⅰ-15除化合物Ⅰ-1、Ⅰ-2和Ⅰ-3外，其余化合物均为首次从该植物中分离得到。2)通过大孔树脂富集该药材有效部位苯乙醇总苷，并采用HPLC测得其所含特征性成分类叶升麻苷的含量可达80%以上。3DPPH自由基清除试验显示紫花列当提取物及其所含的苯乙醇苷类化合物均具有较好的抗氧化能力，其清除DPPH自由基能力接近于抗坏血酸。4采用卡介苗整体致敏、脂多糖离体攻击构建大鼠原代肝细胞免疫性损伤模型，在体外观察类叶升麻苷的保肝作用。

Additionally, 75 volatile chemical constituents have been identified from the petroleum ether extracted part by capillary GC-MS, and the relative percentages of them are determined by GC method. The result showed that the volatile constituents of Musella lasiocarpa mainly includes advanced alkane, aliphatic acid and ester, aldehyde, ketone, aromatic and its heterocyclic compounds. With the compounds that have been isolated and identified from Musella lasiocarpa, the biogenensis pathway of new compound MT-3 is approached. Furthermore, the bacteriostasis of the 6 compounds to Escherichia coil, Pseudomonas aerusinosa, Bacillus megaterium, Sarcina lutea and Bacillus subtilic are determined by flat dilution method.

此外，从其石油醚萃取部分用毛细管色谱法分析鉴定出75种挥发性化学成分，并测定了各组分的相对百分含量，结果表明，地涌金莲挥发性化学成分以高级烷烃、高级脂肪酸酯及醛、酮、芳香和芳香杂环化合物为主；根据从地涌金莲中鉴出和分离得到的化合物，探讨了新结构化合物MT-3的生源代谢途径；采用平板稀释法测定了地涌金莲中六种化学成分对大肠杆菌、绿脓杆菌、巨大芽孢杆菌、藤黄八叠球菌和枯草芽孢杆菌的抑菌作用。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

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如果有任何问题，你可以随时联系我。