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To establish a new method to isolate genomic regulatory sequence using unpurified nuclear extract, here a new strategy was developed based on modified whole genome PCR and electrophoresis mobility shift assay. The principle is as follows. After sonication and ligation with DNA linker, human genomic DNA is amplified and labeled with isotope by whole genome PCR.

为了建立一种利用未经纯化的细胞核粗提物就能从人基因组中快速、大量筛选DNA调节片段的新方法,本研究对原有的全基因组PCR技术进行改进,结合凝胶阻滞实验,建立了筛选DNA调节片段的新方法。

These imply that the statement of ischemia myocardial and anoxia and the function of endothelia cell can be also ameliorated by acupuncture.

CGRP与ET是一对调节血管舒缩作用的重要因子,在人体内构成一个调节心血管系统的环路,共同调节心血管功能。

Due to traverse motion unstable problem of riderless bicycle,simulating dynamics characteristic on human riding bicycle,and adequately using parallel mechanism s merits such as compact frame,great rigidity,rapid response rate and great loading capability,a traverse adjustment method of riderless bicycle based on 2-RHR redundantly actuated parallel mechanism was presented.

针对自行车机器人运动的侧向不稳定性,模仿人骑自行车的动力学特征,充分利用并联机构的结构紧凑、刚度大、响应速度快、承载能力强的特点,提出了一种基于2-RHR型冗余驱动并联机构进行自行车机器人侧向稳定调节的方法,系统地阐述了并联机构侧向调节方式的可行性和优越性。

These results indiacated that tranferring macrophages combinedwith cytokines could be a new adoptive immunotherapy protocol foradvanced cancer,and rTNF used in vivo locally could induce andactivate macrophages to kill tumor cells.Monocytes when activatedunderwent a series of phenotypic and functional changes includingthe expression of IL-2R which may provide an important immunoregu-latory pathway.The presence of lectin-like molecules on thesurface of monocytes and tumor cells may bring theeffector/target cells together,thus facilitating the inductionof apoptosis in target cells by triggering the production ofcytolytic factors and the modification of target of targer cell surface anti-gens.(such as HLA-DR).

综合以上结果可以得出以下结论:转输巨噬细胞并结合细胞因子的联合应用是肿瘤继承性免疫中的又一新的、有潜力的方法;抗肿瘤细胞因子rTNF可通过激活巨噬细胞在体内抑制对其不敏感的肿瘤细胞的生长;人单核细胞在一定条件下激活后,可表达IL-2R,进而一方面增强其自身对IL-2的敏感性,另一方面在体内也具有调节T细胞功能的作用;单核细胞和肿瘤细胞上存在的凝集素类受体可促使两种细胞之间在凝集素介导下相互接近,并诱发单核细胞产生细胞毒因子,以及通过调节靶细胞上表面抗原的表达,促进诱导靶细胞的细胞凋亡。

In some key moments, calling a timeout is no doubt necessary, let the players think about what they should do on court too often sometimes will give the chance to the opponent for a great comeback. If the Lakers desperate want to defeat the Celtics at home, they must show out some strong courage and uprightness, and also a serious-minded attitude. The attitude during the game is really a big gap between the Lakers and Celtics. Take on the Celtics, a tough and high pressure defense is necessary, and Andrew Bynum need to carry on the duty of low post offense, the protection of rebound and defense the paint, using the advantage of the body and make some inside score is a good choice.

过分让球员在场上自我调节反而会丢失很多好时机,而让对手有机可乘对付凯尔特人湖人要拿出血性和拼命认真的态度,而现在的湖人和凯尔特人就是态度上就有很大的差距对付凯尔特人,需要做到高强度防守,而拜纳姆也要负担起强攻内线、保护篮板、防守禁区的重任,多用身体优势强攻内线是一个不错的选择三角进攻在进攻端一定要灵活执行,以加索尔为轴心

The battle of the Bobs may not be completely over, but it has certainly been suspended.

因此,至今为止,首要的问题是,任何民主党人的经济观点常常取决于他们的立场,里奇和鲁宾,他们分属于左翼和中间派,亦即政府调节和市场调节。

Unscrew the adjustment screw ring, stretching from the exposed screw people like a thin tool bar, you can toggle adjustment ring on the teeth, so that adjustment ring turning left.

拧下调节圈固定螺钉,从露出的螺孔伸人一根细铁棍之类的工具,即可拨动调节圈上的轮齿,使调节圈左右转动。

Aiming at the lack of the approach of education of the ability of emotion regulation of junior high school students, using for reference of the reasonable composition of emotion regulation, self-education, combining the unsteady actuality of emotion development of junior high school students, adopting the manner of the research-oriented study toward the ability of emotion regulate of junior high school students have proceeded to the education experiment, which will overcome the deficiency and limitation of current research-oriented study only paying attention to person and nature, person and society, and adds the contents of the person and self.

针对以往初中生情绪调节能力培养途径研究的不足,借鉴情绪调节、自我教育的合理成分,结合初中生情绪发展不稳定的现实,采用研究性学习方式对初中生情绪调节能力进行了培养实验,克服现有研究性学习只关注人与自然、人与社会的不足和缺陷,增加了人与自我的内容。

Unscrew the adjustment screw ring, stretching from the exposed screw people like a thin iron tool, you can toggle adjustment ring on the teeth, so that adjustment ring turning left.

拧下调节圈固定螺钉,从露出的螺孔伸人一根细铁棍之类的工具,即可拨动调节圈上的轮齿,使调节圈左右转动。

To determine the pilot dimensions on which the kinetic stabilization adjustment design of ejection seat depends,regression analysis of 292 pilots' dimensions and their combined gravity centers of pilot-seat systems were conducted. Stature and body weight were determined to be the depending parameters, and the regression equations were derived.

为确定火箭弹射座椅动力稳定调节方法设计所应依据的人体参数,对292例飞行员的人体参数及人椅系统组合重心数据进行了回归分析研究,综合确定了以体重和身高作为调节依据参数,并求出了该参数与人椅系统组合重心的回归方程。

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Finally, according to market conditions and market products this article paper analyzes the trends in the development of camera technology, and designs a color night vision camera.

最后根据市场情况和市面上产品的情况分析了摄像机技术的发展趋势,并设计了一款彩色夜视摄像机。

Only person height weeds and the fierce looks stone idles were there.

只有半人深的荒草和龇牙咧嘴的神像。

This dramatic range, steeper than the Himalayas, is the upturned rim of the eastern edge of Tibet, a plateau that has risen to 5 km in response to the slow but un stoppable collision of India with Asia that began about 55 million years ago and which continues unabated today.

这一引人注目的地域范围,比喜马拉雅山更加陡峭,是处于西藏东部边缘的朝上翻的边框地带。响应启始于约5500万年前的、缓慢的但却不可阻挡的印度与亚洲地壳板块碰撞,高原已上升至五千米,这种碰撞持续至今,毫无衰退。