调素

In whole test process, to compared with control group, hepatic hepcidin gene expression of test group on different phases had obviously differences with retinoic acid was administered. On early phase of test group , relative gene expression contents of hepcidin were gradually fall and the lowest level was on 8th、9th、10th day, then the hepcidin level gradually was ascendent. The curve was like a cavate parabola.

在实验周期内，模型组与对照组比较，肝脏铁调素基因表达在维甲酸灌胃的不同时间，存在明显差异：早期5、6、7天铁调素基因相对表达量逐渐下降，在第8、9、10天达到较低值，随后有回升，呈现凹陷的抛物线变化趋势；而对照组基本处于水平的轻微波动变化。

This research is based on our previous successful cloning of a completely novel calcium metabolism related protein—calmodulin-like protein from pearl oyster and calmodulin. The main purpose of this research is to find out the function of CaLP on the cell level and compare functions of these two proteins.

为进一步揭示贝类生物矿化机理，本研究以目前我国用于生产海水珍珠的主要贝类合浦珠母贝为研究材料，以本实验室已克隆出的新蛋白钙调素类似蛋白(calmodulin-like protein，CaLP)为研究对象，对CaLP在细胞水平上的功能进行了进一步的研究，并将其与本实验室已克隆出来的另一钙代谢相关蛋白钙调素(calmodulin，CaM)进行了比较。

Under drought stress, 5mmol/L and 10mmol/L Ca~(2 ) test can increase thecontent of endogeny calcium, then improve the drought resistance of strawberry. And5mmol/L Ca~(2 ) test can increase the content of endogeny CaM, because CaM adjustinner enzyme activity, improve the drought resistance of strawberry.

在干旱胁迫下，5mmol／L和10mmol／L钙处理能提高草莓内源钙含量，从而提高草莓的抗旱性。5mmol／L钙处理能提高草莓内源钙调素含量，是由于钙调素调节了体内酶的活性，从而增强了草莓的抗旱性。

According to experimental results and etiopathogenisis and pathology of osteoporosis , and analyzing review investigation on hepcidin、associations of bone metabolism and iron metabolism and relations of ferri ion and calcium ion,we can make conclusions as follows: 1、We applied single manmade intervention approach to induce osteoporosis model in this study, there was no other influential factor. In the termination of test , rats bone density and bone histological anatomy indicated that osteoporosis model was creditable,meanwhile hepatic functional enzyme and hepatic microtome section demonstrated that hepatic function had noci-influence induced by medicinal herbs resource. Accordingly, we could believe that hepatic hepcidin gene expression and serum hepcidin contents can represent internal iron metabolism changes at selected test time. 2、In this investigation, different findings between test group and control group indicated that:according to changes of hepcidin gene expression measured by RT-PCR and changes of serum hepcidin contents determined by ELISA kit , there were correlations between hepcidin and rat osteoporosis model induced by retinoic acid. 3、Under the condition of this investigation,there was a interactive hypothesis as follows: Hepcidin iron metabolism←→ferriion←→calcium ion←→osteoporosis.

根据本次实验结果，对骨质疏松的病因病理的认识及铁代谢与铁调素、骨代谢与铁代谢关系、铁离子与钙离子的关系系列研究的回顾，经分析得出如下结论：1、本研究骨质疏松模型采用单一人工干预方法，没有其他影响因素；实验结束时大鼠骨密度、骨组织学检查表明骨质疏松形成，同时肝脏功能酶和HE切片显示肝脏功能未受药源性产生明显损伤性影响；因此，同期不同时间组大鼠肝脏铁调素基因表达、血清铁调素含量可以代表体内铁代谢不同时间的变化。2、在本研究中，模型组与对照组研究结果的差异表明：铁调素基因RT-PCR变化、血清铁调素ELISA测定含量变化与维甲酸制作的大鼠骨质疏松模型形成有实验相关性。3、在本研究平台下，形成了一个互相影响的假设图：铁调素←→铁代谢←→铁离子←→钙离子骨质疏松。

The GFP-OsCaM61 fusion protein was membrane-associated whereas OsCaM61-GFP was mainly detected in the nucleoplasm.

体外活性测定实验表明，0sCAM61可以激活钙调素的靶酶，说明它具有钙调素的功能。

We consider that the edge toneme peeling is the main reason of prosodic phenomenon in the concatenation of continuous speech units.

我们认为：汉语连续发音时各语音单元的组合导致相应边缘调素的脱落，其韵律现象产生的根本原因在于边缘调素的脱落。

The concentrations of free calcium ion, calmodulin and cyclic adenylic acid in the rat brain were determined.

结果显示，模型大鼠脑内游离钙离子、钙调素和环核苷酸含量降低，阿米替林可使模型大鼠脑内游离钙离子、钙调素浓度升高，而对环核苷酸则无影响。

Cell membrane nonpermeable inhibitor of calmodulin, W7_agarose, and the anti_serum of calmodulin could decrease the cytosolic calcium level. The results show that the endogenous extracellular calmodulin may play an important role in maintaining and increasing the cytosolic calcium level in pollen grain cell.

不能透过细胞膜的钙调素拮抗剂W7_agarose和植物钙调素抗血清处理都可以使花粉细胞质中游离钙离子浓度降低，表明内源细胞外钙调素可能在维持和促进花粉细胞质中游离钙离子方面具有重要作用。

Cell membrane nonpermeable inhibitor of calmodulin -W〓-agarose- and the anti-serum of calmodulin can decrease the cytosolic calcium level, the results show that the endogeneous extracellular calmodulin may play an important role in maintaining and accelerating the cytosolic calcium level in pollen grain cell.

不能透过细胞膜的钙调素抗血清和大分子钙调素拮抗剂W〓-agarose处理后细胞内钙离子水平降低，免疫前血清对花粉细胞内钙离子浓度没有明显影响；进一步支持了上述结果，并且表明内源细胞外钙调素在维持和提高细胞内钙离子浓度方面发挥作用。

The highest expression level in different tissues was found at full blossom, followed by before blossom and after blossom. Calmodulin mRNA accumulated mainly in ovaries and fruitlets, and reached the highest at blossoming.

在细胞水平上，钙调素mRNA的表达主要集中于细胞的胞间隙和胞间层及细胞核；钙调素mRNA的表达水平在各组织不同发育阶段也表现为盛花时最高，花前稍高于花后。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。