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Depositing cells were incubated in high-sugar DMEM culture medium containing 15% fetal bovine serum, 100 U/mL penicillin, and 100 U/mL Streptomycin, then cells were seeded in a 25 T culture bottle after cell density was adjusted to 1×109 L-1, and incubated at 37 ℃, 0.05 volume fraction CO2 with saturated humidity. After 72 hours, the culture medium was replaced, and red blood cells and other nonadherent cells were removed. Cells began to passage at 70%-80% confluence.

实验方法:穿刺抽取15 mL脐带血,密度梯度法分离吸取分层界面的白色环形絮状物,其中富含单个核细胞,离心后沉积细胞用含15%胎牛血清、100 U/mL青霉素、100 U/mL链霉素的DMEM高糖培养基悬浮,调整细胞密度为1×109 L-1接种于25 T培养瓶内,放置在37 ℃、体积分数为0.05的CO2饱和湿度培养箱中培养,72 h后更换培养基,去除红细胞及其他未贴壁细胞,至70%~80%融合时传代。

The Federal Reserve said consumer borrowing rose at a seasonally adjusted 2.4% annual rate in February to $2.54 trillion, half the 4.9% pace recorded in January. Credit-card borrowing rose at a 5.9% pace in February, but demand for auto loans and other kinds of nonrevolving debt slowed to a 0.4% rate.

据美国联邦储备委员会公布的数据,2月份美国消费者贷款额达到2.54万亿美元,经季节性因素调整后增幅折合年率为2.4%,此前1月份的增幅为4.9%。2月份的信用卡借款增幅为5.9%,而汽车贷款和其他类别的非循环借款增幅却跌至0.4%。

The prevention complication occurs in the morning, is mainly the blood pressure blood sugar blood fats long-term stability control under the quite good condition, does not smoke, does not drink the protosalt low fat diet, regularly to hospital inspection, adjustment treatment blood sugar normal range, empty stomach blood sugar 60 to 100 milligrams each liter.

防治并发症上午发生,主要是将血压血糖血脂长期稳定控制在比较好的状态下,不抽烟、不喝酒低盐低脂饮食,定期到医院检查,调整治疗血糖的正常范围,空腹血糖60至100毫克每升。

Healthy kunming mice mate to produce embryo. sacifice prognant mice at 14dpc, make preparation of fetal liver and born marrow. then proceed 40%-70% percoll gradient seperation to obtain nucleated erythroid cells. resuspend the purified erythroid cells in DMEM medium without serum, adjust cell concentration to 5×10〓.

正常健康昆明小鼠交配,待胚胎发育至14天时,处死怀孕母鼠,取胎鼠肝脏,用另一组成年小鼠制备骨髓;然后应用40%-70%Percoll梯度分离法分离胎肝和骨髓中的有核红细胞,纯化后的细胞用无血清DMEM培养基悬浮,调整细胞浓度为5×〓/ml。

Results indicate that radial distance of the bushing from the rotor center and the radius of the hemispherical bushing significantly affect wear rate,and minimzing both two values will result in lowest wear for micro-rotor systems in MEMS.However,it is noted that the bearing radius sets a lower limit on the hemispherical bushing radius since the bushings are located beyond the rotor inner radius.It is desirable to reduce their effects on friction and wear on micro-rotors by regulating the radius of the bushing and the radial distance of the bushing from the rotor center within permissive range of micro-fabrication.Severe wear exists with increasing applied load and rotating speed of the rotor.Stress distributions in the contact region are different from Hertz solutions due to the convergence stresses apearing on the uncontinuous curvature line and the contact pressures display symmetrical parabola in the contact region.

结果表明:在MEMS微转子系统中,微转子轴衬偏心距离和转子轴衬半径影响线磨损率,以两者减至最小为较理想,但须注意设计中轴承半径的限制,并使轴衬偏心距离大于轴承内半径;在转子加工制造许可的范围内,可以通过调整转子轴衬的半径和偏心距离来减少摩擦磨损对微电机的影响,随着转子所承受载荷和转子旋转速度的增加,其磨损严重;接触区的应力分布不同于经典Hertz解,这是由于应力集中出现在不连续的曲率线上,且接触区的接触压力呈现轴对称抛物线状分布。

Orthodontic braces usually need adjusting every four to six weeks.

牙套矫正,通常需要调整,每4至6个星期。

Furthermore, monitoring the changes in Raman spectra of the electrochromic films with bleach-coloration states enables the discussion of the mechanism of colouration.

此外,本专题制作的影像显示元件,其操作电压范围在-4V至+4V间,可透过调整驱动电压大小来改变颜色的深浅,提高元件的视觉对比。

When properly adjusted, the flame-out light should come on within 10 to 20 seconds after a flame out.

当适当的调整后点火10至20秒后火焰就因该出现。

The new three-pot displaces 1.35L and can generate between 120 and 180 horsepower, depending on tuning.

新的三锅取代1.35L和可产生120至180马力,根据调整。

Overproduced YlyA existed as inclusion body, while the induction was carried out with 0.5mM IPTG, at 37℃ for 3h; Modified the induction condition to 0.05mM IPTG, at 25℃ for 5h, a portion of His-tagged YlyA turned into soluble; After purification treatment, the concentration of the fused protein reached to 204.2119μM.

结果表明pNG252中ylyA的插入方向正确,序列无突变;用0.5mM IPTG ,37℃诱导3h时,YlyA虽高效表达,但为包涵体形式;调整诱导条件至0.05mMIPTG,25℃,5h时,高效表达的YlyA部分转为可溶性蛋白。

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However, as the name(read-only memory)implies, CD disks cannot be written onorchanged in any way.

然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口,替代木托盘,免薰蒸,符合欧盟、北美各国对出口货物包装材料的法令要求;喷涂钢托盘适用于重载上货架之用,托盘表面根据需要制作成平板状、波纹状及间隔形式,满足不同的使用要求。

A single payment file can be uploaded from an ERP system to effect all pan-China RMB payments and overseas payments in all currencies.

付款指令文件可从您的 ERP 系统上传到我们的电子银行系统来只是国内及对海外各种币种付款。