诱导的

OBJECTIVE: To establish an inducing system for differentiation of bone marrow mesenchymal stem cells into chondrecytes in two-dimensional culture in vivo, to analyze the best concentration of transforming growth favor beta-1 (TGF-β1) to induce the differentiation and the correlated influence factors for the directional differentiation, and to observe the changes of cell form and phenotype.

目的：建立二维培养条件下骨髓间充质干细胞分化为软骨细胞的诱导体系，分析转化生长因子β1作为诱导条件的最佳浓度，以及诱导骨髓间充质干细胞定向分化为软骨细胞的相关影响因素，观察细胞诱导后的细胞形态及表型变化。

OBJECTIVE: To establish an inducing system for differentiation of bone marrow mesenchymal stem cells into chondrecytes in two-dimensional culture in vivo, to analyze tbe best concentration of transforming growth favor beta-1 (TGF-β1) to induce tbe differentiation and the correlated influence factors for the directional differentiation, and to observe the changes of cell form and phenotype.

目的：建立二维培养条件下骨髓间充质干细胞分化为软骨细胞的诱导体系，分析转化生长因子β 1作为诱导条件的最佳浓度，以及诱导骨髓间充质干细胞定向分化为软骨细胞的相关影响因素，观察细胞诱导后的细胞形态及表型变化。

Based on the previous literatures, the following major innovation works were carried out in this dissertation:(1) The derivatization reaction conditions of ephedrine and pseudoephedrine with sensitive derivation reagent in aqueous system were studied and two new methods were developed for the assay of them by microemulsion electrokinetic chromatography and micellar electrokinetic chromatography with LIF detection. The sensitivity and analysis times were greatly improved compared with the previous reports;(2) A new method of CZE with indirect LIF detection was developed for the simultaneous determination of six coumarin compounds (esculin, esculetin, isofraxidin, genistein, naringin and sophoricoside) with fluorescein as the probe. The proposed method enlarges the application range of LIF detector, and provides new approach for the analysis of certain compounds difficult to derivatize;(3) Based on the above research, an MEKC with indirect LIF detection method for the simultaneous determination of adenine and guanine in DNA extracts from fungus, maize and soybean was established;(4) A new method for the investigation of the complexes formed between human serum albumin and ampicillin sodium under the simulated physiology conditions using laser light scattering technique was developed.

该论文在综述前人工作的基础上，开展了如下未见文献报道的创新性的研究工作：(1)使用灵敏的衍生试剂对麻黄碱和伪麻黄碱在水体系中的衍生反应条件进行了系统研究，建立了微乳电动色谱-激光诱导荧光检测法和胶束电动色谱—激光诱导荧光检测法测定麻黄碱和伪麻黄碱的灵敏分析新方法，与以前的报道相比，灵敏度和分析时间均有很大改善；(2)使用荧光素钠作为背景荧光试剂，建立了同时分析测定六种黄酮类化合物(秦皮甲素、秦皮乙素、异秦皮定、染料木素、柚皮苷和槐角苷)的毛细管区带电泳—间接激光诱导荧光检测新方法，扩大了激光诱导荧光检测的应用范围，对难衍生化合物的分离分析提供了一种新思路和新途径；(3)以荧光素钠作为背景荧光试剂，建立了一种用于同时测定食品提取DNA中的腺嘌呤和鸟嘌呤含量的胶束电动色谱—间接激光诱导荧光检测新方法；(4)用动态和静态激光散射法研究了模拟生理条件下人血清白蛋白与氨比西林钠盐相互作用所形成的复合物，为药物分子与蛋白质的相互作用、药代动力学等的研究提供了一种新思路。

Through two steps, advancing cultivation and inducing differentiation, principium founded tissue culture system of fodder beet with explant from footstalk, so established foundation for inducing polyploid fodder beet with tissue culture and enlarging reproduction.

通过预培养、诱导分化两步法，初步建立了以叶柄为外植体诱导不定芽再生的饲用甜菜组织培养体系，为进一步利用组织培养诱导饲用甜菜多倍体及多倍体扩繁奠定了基础。

The results showed that there were different and heterosis in rates of induced haploid among material of induced haploid.

结果表明，不同诱导材料的单倍体诱导率存在较大差异，单倍体诱导率可能存在超亲优势。

BA and GA3 in proper concentrations were the effective hormones to induce the germination of germ tube and the germinating rate was 3.3%. When treated with 6-BA + GA3 + IBA, the germinating rate doubled compared with single hormone treatments. However, the germ tube didn't form haustorium afterwards.

结果如下： 1、适当浓度的6-BA和GA3能够诱导肉苁蓉种子萌发形成芽管，芽管萌发率仅为3.3%，6-BA + GA3 + IBA组合使种子芽管萌发率提高2倍，激素只能诱导肉苁蓉种子萌发芽管，不能进一步诱导肉苁蓉种子形成吸器。

Then, with some examples, the problems of directional indicator design for underground space are discussed systematically from angles of space inductive design and indicator sign system.

本文从理论上阐明了地下空间方向诱导设计的必要性，介绍了定位定向的相关理论，然后结合实例，从空间诱导设计和标示系统诱导设计两大方面对地下空间方向诱导设计的问题进行了较为系统的探讨。

Based on the detailed analysis of a variety of factors affecting stability of taxol production in Taxus cells, it was discovered that initial cell density and the relevant concentrations of substrates, elicitors and precursors significantly affected stability of taxol production; farther study showed that addition of elicitors at appropriate cellular state was the key to steadily improve taxol yield, especially, the proper cellular state was at the end of lag phase and at the beginning of the stationary phase, which was also proved by the cellular ultrastructure.

对影响红豆杉细胞紫杉醇产量稳定性的各类因素进行详细分析，发现细胞的接种密度以及与之相适应的培养基的基质浓度、诱导子浓度和前体浓度是影响紫杉醇产量稳定的重要因素；进一步研究发现，选择细胞处于合适的状态时加入诱导子是稳定提高紫杉醇产量的关键，细胞合适状态特别是延迟期末和稳定期初最合适，细胞状态的超微结构观察结果也证明了这一点；同时研究发现采用合适的同步化处理获得的细胞初始周期时相加入诱导子与紫杉醇产量稳定密切相关，且以低温同步化处理得到的细胞周期的分裂中期相的细胞加入诱导子不仅大大提高了紫杉醇的产量，而且诱导子加入时间大大缩短，有利于大大提高紫杉醇生产率。

TGF-β1 is the major factor of chondrogenesis while 10 μg/L is the best inducing dosage.

MSCs在一定诱导条件下可以向软骨细胞分化，转化生长因子β1（TGF-β1）是诱导MSCs向软骨分化的关键性因素，10 μg/L是其最佳诱导剂量。

The investigated potassium carboxylates included: monocarboxylate potassium acetate, dicarboxylate potassium tartrate (K2Tart), tricarboxylate potassium citrate (K3Cit), and tetracarboxylate dipotassium of ethylenediaminetetraacetic acid (K2EDTA). KOAc can only induce calcium oxalate monohydrate, while K3Cit and K2EDTA induce COM and calcium oxalate dihydrate. The ability of multicarboxylates to induce COD formation was increased with increasing their concentrations.

加入一元醋酸钾只生成一水草酸钙晶体；三元柠檬酸钾(K3Cit)和四元乙二胺四乙酸二钾(K2EDTA)可诱导二水草酸钙形成，且随着其浓度增加，对COD的诱导能力增加，而二元酒石酸钾(K2Tart)同时诱导了COM， COD和三水草酸钙生成。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。