诱导的

The results demonstrated that the intracellular pH change was different when the Hela cells was treated with different anti-tumor drugs, some would caused intracellular acidification, some would caused ntracellular basification, and some would barely caused pH change. The single living Hela cells uptaken with the ratiometric pH nanosensor was also imaged with laser confocal microscope, which were treated with anti-tumor drugs.

通过利用该纳米传感器对硫酸长春新碱诱导后的Hela细胞内pH变化的活体、原位、实时监测，发现在硫酸长春新碱诱导引起凋亡的Hela细胞中，细胞内的pH值由诱导前的7.11酸化为6.51，并且在一定的浓度和时间范围内，硫酸长春新碱诱导后细胞内的酸化率与诱导药物的浓度和诱导时间成正相关关系。

Objective To compare antibacterial activity of antibacterial protein in the Musca domestica larvae by five inducements and observe the effect of the different inducements.

目的 比较5种诱导源所诱导产生家蝇幼虫抗菌蛋白的抗菌活性，观察不同诱导源对家蝇幼虫抗菌蛋白产生的诱导效果。

Esults When MSCs were cultured with HGF, FGF-4 and OSM, cuboidal morphology, which was a characteristic of hepatocytes, was observed, and the differentiated cells also expressed marker genes specific to liver cells in a time-dependent manner.α-fetoprotein was expressed on day 10, and CK18, ALB and TAT were detected on day 20, which was in consistent with the immunofluoresence results.

T-PCR和免疫荧光检测结果显示，AFP在诱导初期表达，在诱导后期表达下降；CK18、ALB和TAT的表达与诱导时间呈线性关系，第20天达到表达高峰；诱导20 d后免疫荧光检测，AFP、CK18、ALB和TAT均为阳性；诱导20 d细胞的PAS糖原合成反应呈阳性，即具备糖原合成和储存的肝细胞特有功能。

METHODS: The third passage of BMSCs and AMSCs at a density of 5×10^7 L^(-1) were divided into two groups. Cells in the induction group were incubated in 1.5 mL of osteoinductive medium, supplemented with 10^8 mol/L dexamethasone, 10 mol/L β-glycerophosphoric acid, and 50 mg/L vitamin C. Cells in the non-induction group were not treated with osteoinductive medium.

选取第3代骨髓间充质干细胞和脂肪间充质干细胞，按5×10^7L^(-1)接种后各分为2组：诱导组加入含10^8mol/L地塞米松、10mol/L β-甘油磷酸、50mg/L维生素C的成骨诱导培养基1.5mL，未诱导组不加入成骨诱导培养基。

Bone marrow-derived mesenchymal stem cells are capable of chondrogenesis, making them a possible source of cells for injectable cartilage tissue engineering. There exist different ideas on the ability of mesenchymal stem cells's chondrogenesis in monolayer culture. Because of this, the effect of adult rabbit's bone marrow-derived mesenchymal stem cells chondrogenesis in monolayer culture was studied. The mesenchymal stem cells was isolated from adult rabbit's bone marrow and monolayer cultured. TGF-β1, Vit-C and Dexamethasone were used. Immunohistochemistry analyses and histological staining of H-E, Methylaniline blue and Alcian blue were performed to identify the expression of collagen type Ⅱ and cartilage associated matrix. The results showed that the induced cells expressed and produced collagen type Ⅱ and cartilage associated matrix. This suggests that the differentiation of adult rabbit's marrow-derived mesenchymal stem cells into chondrocyte in monolayer culture is feasible and may be induced by TGF-β1, Vit-C and Dexamethasone.

骨髓基质干细胞的软骨分化潜能使其可能成为可注射组织工程化软骨研究的种子细胞，为探讨体外培养的骨髓基质干细胞在平面诱导条件下软骨分化的可行性，我们进行下列实验：获取并体外平面培养成体兔骨髓基质干细胞，应用TGF-β〓、Vit-C和地塞米松对其软骨分化诱导，诱导后的骨髓基质干细胞行细胞爬片组织学和Ⅱ型胶原免疫组化，结果证实，诱导后骨髓基质干细胞可分泌Ⅱ型胶原，组织学染色可见类似于软骨细胞，由此证明体外培养的骨髓基质干细胞在平面诱导条件下可以软骨分化，其软骨诱导因子为TGF-β〓、Vit-C和地塞米松。

Firstly, callus induction and subculture for Mikania micrantha were studied systematically. The research on callus induction showed that: Shoot tip has higher induction rate than other explants ; At 25℃ and dark regime, the callus was better cultivated on Murashige and Skoog medium (pH5.8) containing 2.0mg/L 2,4-D and 1.0mg/L KT.

首先，对微甘菊愈伤组织诱导和继代增殖培养进行系统研究，愈伤组织诱导研究表明：外植体中芽尖诱导率最高，在含2，4-D2.0mg／L、KT1.0mg／L、pH5.8的MS培养基上25℃黑暗条件下愈伤组织诱导效果较好。

Expanded MSCs were induced to differentiate into nerve cells withthree different treatment protocol in the prensent study . On the following day, the medium was replaced with preinduction medium consisting of DMEM, 20% FCS, and 10 ng/ml basic fibroblast growth factor. After 24 h, the preinduction medium was removed, the cells were washed twice with PBS, and neuronal induction medium containing DMEM supplemented with 2% DMSO and200 m bu-tylated hydroxyanisole was added. In later experiments, we used DMEM with 5 mM p - mercaptoethanol or sulfo - glycerine as an alternative neuronal induction medium for the same incubation.

将待诱导分化的MSCs按0.4x10~6／ml接种于事先放置有消毒盖玻片的六孔板内制备细胞爬片，分别采用三种不同的方法诱导第2代至10代的MSCs向神经细胞分化，即方法1先用含bFGF的培养基进行预诱导，之后再用含叔丁基对羟基茴香醚，和二甲亚砜的无血清培养基进行诱导；方法2和方法3用B-巯基乙醇和硫代甘油进行预诱导，然后不同浓度的上述物质进行正式诱导。

The adopted lactose concentration for induction, the time point of induction, the duration of induction and the dynamics of uricase expression were optimized and studied in detail by shake flask experiment.

通过摇瓶试验对诱导所采用的乳糖浓度，诱导时机和诱导持续时间进行了优化，并考察在乳糖诱导下的目的产物表达动力学，随后在5 L发酵罐上进行扩大化培养以验证摇瓶优化的结果，进一步将乳糖作为诱导剂应用于高密度发酵过程。

1、Cur inhibits K562 cells growth and induces cell apoptosis may be correlated with the down-regulation of p210~、inhibition of protein tyrosine phosphorylation and the signaling molecules such as p-Erk1/2、c-myc which are relevant with cell growth and apoptosis; 2、Cur synergizes STI571 to inhibit K562 cell growth and induce cell apoptosis may be correlated with the down-regulation of p210~、inhibition of protein tyrosine phosphorylation and the signaling molecules such as Hsp90、PKC which are relevant with cell growth and apoptosis; 3、Cur reverses the resistance of K562/G01 cells to STI571, and synergizes STI571 to inhibit K562/G01 cell growth and induce cell apoptosis; 4、Cur inhibits human originated CML CD34~+ cell growth、induces cell apoptosis, and enhances STI571 to down-regulate the expression of p210~, finally inhibit cell growth and induce cell apoptosis.

从以上实验结果我们得出如下结论： 1、Cur抑制K562细胞增殖、诱导细胞凋亡的作用可能与其下调p210~、蛋白酪氨酸磷酸化水平以及抑制下游p-Erk1/2、c-myc等信号分子有关； 2、Cur协同STI571抑制K562细胞增殖、诱导细胞凋亡的作用可能与其下调p210~、蛋白酪氨酸磷酸化水平以及抑制Hsp90和下游PKC等信号分子有关； 3、Cur可逆转K562/G01细胞对STI571的耐药性，并与STI571协同抑制K562/G01细胞增殖和诱导凋亡，其抑制K562/G01细胞增殖、诱导细胞凋亡的作用可能与其下调p210~、蛋白酪氨酸磷酸化水平以及抑制下游Procaspase-3和NF-κB等信号分子有关； 4、Cur可抑制来源于CML患者骨髓的CD34~+细胞的增殖并诱导其凋亡，还可协同STI571下调CML CD34~+细胞p210~表达，进而协同抑制细胞增殖、诱导细胞凋亡。

Methods Marrow-derived mesenchymal stem cells were isolated and purified from mouse femoral bone and shinbones using differential adherent methods. Cells at the third passage were induced by 20% FBS in conditioned medium, conditioned medium alone, 20% FBS or 10% FBS alone respectively. Mouse aortic smooth muscle cells were cultured as the positive control. Levels of mRNA and protein expression of myoeardin and several smooth muscle cells marker genes were determined by immumofluorescenee, RT-PCR and Western blot before and 3, 7, 10, 14d after the induction. The presence of smooth muscle myofilaments was detected by using transmission electron microscope.

采用伞骨髓贴壁法分离骨髓问充质干细胞，CM联合20%FBS诱导骨髓间充质干细胞，同时设立持续10%FBS、单20%FBS及单CM诱导下的骨髓间充质十细胞对照组和SMC阳性对照组，分别于诱导前及诱导3、7、10和14 d时观察细胞形态的变化，并在相应的时间点用免疫荧光法、逆转录聚合酶链反应法、Western blot半定量分析法榆测myocardin以及SMC表面各种标志基因的表达变化，用透射电镜检测诱导后细胞内肌丝存在以此来证实诱导分化成功。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

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如果有任何问题，你可以随时联系我。