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The direct incorporation of U-13C glucose was estimated by the intensity increase of m/z to F(F was parent fragment, and n was the carbon number in the fragment), while the total isotope incorporation from the added 13C could be calculated according to the abundance ratio increment summation from m/z (Fa+1) through (Fa was the fragment containing all original skeleton carbons, and T was the carbon number in the amino acid molecule). The 13C enrichment in the target compound was expressed as atom percentage excess, and that of D-amino acid needed to be corrected by the coefficient of hydrolysis-induced racemization. The 13C enrichment reflected the carbon turnover velocity of individual amino acid enantiomers, and was powerful to investigate the dynamics of soil amino acids.

外加葡萄糖直接合成氨基酸的比例可利用质谱碎片的相对强度变化来评价(n为质谱碎片F中含有的碳原子数目);而源于葡萄糖的13C同位素在氨基酸分子中的富集程度是所有同位素峰相对强度变化的总和。13C在目标化合物中的富集比例用原子百分超评价,D氨-基酸的APE还需进一步利用水解诱导的外消旋化系数校正。13C在目标化合物中的富集程度可反映各氨基酸手性异构体的碳循环速率大小,是研究土壤氨基酸动态变化的有力工具。

The results show that the increasing Marangoni number will lead to more nonlinear distribution of temperature field, but streamfunction of the cell center; vorticity on the wall and peak value of velocity diminish first, then increase, finally lose stability. The thermocapillary convection in the whole liquid zone appears to get stronger along with the augmentation of Prantal number. The effect of increasing of Biot number on temperature and flow fields is not obvious although the convection is strengthened a little. As increasing the aspect ratio A, thermocapillary convection induced by temperature gradient and liquid viscosity becomes stronger.

结果表明:温度场的非线性分布随着Ma数的增大而加剧,但对流涡胞中心流函数、壁面涡量和轴向、径向速度峰值并不象温度场随着Ma数的增大而单调增大,而是先减小,然后增大,最后失稳,表明Ma数对热毛细对流的影响比较复杂,针对不同的物理模型,会导致完全不同的流态;Pr数愈大,流体的热扩散能力在粘性扩散与热扩散能力之比中愈占支配地位,当Ma数一定时,热毛细对流随Pr数的增大在整个液体区域内有增强的趋势;Bi数增大,热毛细对流有所增强,但不甚明显;A的增大使得由温度梯度和流体粘性诱导的热毛细对流随之增强。

In chapter Ⅶ the perturbation theory on the optical ac Stark effect (see chapter Ⅳ) is applied to study the NMR spectroscopy in paramagnetic and diamagnetic systems.

在第七章,我们将第五章中应用于ESR系统的光移动的处理方法推广应用到NMR体系,探索研究了在激光场的作用下,顺磁体系和具有简并激发态的逆磁体系的NMR谱的变化,提出关于激光诱导的NMR频移的理论。

In addition, it was also found that the sulfated α-D-1, 3-glucans with higher degree of substitution had suppressive effects on dimethylbene-induced ear shell swell of mice, and possessed antivirus (HSV-1 and Influenza A) activities in vitro, suggesting that the sulfated glycans have potential usages in the release of inflammation of nerve system and the infection of some virus.

另外,还发现,具有较高取代度的硫酸化灵芝多糖对二甲苯诱导的小鼠耳壳肿胀,单纯疱症病毒(HSV-1)对Vero细胞的感染及A型流感病毒对MDCK细胞的感染均有一定的抑制作用,说明硫酸化灵芝多糖对神经系统炎症及病毒感染具有潜在的治疗作用。

The LD50 of acute toxicity to mice and chronic accumulated toxicity to bandicoots of crude and processed Radix Aristolochice were observated.Intestinal and myokinetic influence of normal and revulsive hyperactive gastrointestinal motility of mice induced by neostigmine were observated by giving aqueous extract and alcoholic extract of crude and processed Radix Aristolochice.Relieving pain and eliminating inflammation to mice also were observated .

采用小鼠急性毒性和大鼠蓄积性毒性方法观察青木香炮制前后对小鼠急性毒性LD50和对大鼠的慢性蓄积性毒性作用;观察了青木香炮制前后的水煎剂和醇提物对正常和新斯的明诱导的胃肠运动机能亢进小鼠肠肌运动的影响;并观察了对小鼠的镇痛和抗炎作用。

Minocycline represses diabetes-induced inflammatory cytokine production, reduces the release of cytotoxins from actiated microglia, and significantly reduces measurable caspase-3 actiity within the retina.

二甲胺四环素抑制糖尿病诱导的炎性细胞因子的产生,降低来自于活化的小神经胶质细胞的细胞因子的释放,同时显著降低视网膜内可检测的caspase-3的活性。

Gangliosides antagonized the apoptosis induced by corticosteron, and the underlying mechanism is related to activiation of neuronal calcium signal cascade, promoting neurotransmitter release. Gangliosides also have an ability to promote the neuronal filopodia formation and may have a antidepressive-like neuroprotecive effect. 2. Theory study: We explained the clinical symptoms showed in the syndromes of deficiency of kidney-Yang and deficiency of kidney-Yin with modern scientific technical terms. Moreover, we supposed the location of kidney of traditional Chinese medicine may be in the hippocampus but not in the hypothalamus, which is also the centre of learning and memory.

鹿茸有效成分能够拮抗皮质酮诱导的神经细胞凋亡,其机制与影响钙信号途径、促进神经递质释放有关,鹿茸有效成分还能促进神经细胞丝状伪足生长,并且可能具有抗抑郁药样的神经保护作用。2、理论研究:用现代科学语言对肾阳虚证和肾阴虚证临床症状的病理生理机制进行合理的解释,提出中医肾脑相关理论实质假说,推测补肾中药的调控点应当在下丘脑—垂体—肾上腺轴的高级中枢海马,而非下丘脑。

What we focused on were the photo-induced selective fissions ofα and β bonds, properties of low-lying electronic states, and mechanisms of photochemical reactions for the above molecules, which are the leading research fields enjoying the interest and attention of chemists.

在本论文中,我们应用CASSCF、MP2、MR-CI、DFT等精确的量子化学计算方法,以BrCH〓COCl、CH〓COCOOH、CH〓COCN、HCOCN、CH〓CHS、〓CS和HCSCN等体系为对象,对目前国际上感兴趣的前沿课题——光诱导的α和β键的选择性解离、低电子激发态的结构和性质以及光化学反应机理等进行了详细的理论研究,并得到了具有重要意义的结果。

objective to investigate staining methods for two-dimensional gel(2-de)electrophoresis in multidrug resistance of gastric cancer.methods cultured vincristine-resistant human gastric cancer cell line sgc7901/vcr and its parental cell line sgc7901.variant amount protein of those cells were separated by 2-de.gels were stained with silver nitrate or colloidal coomassie brilliant blue,and scanned by image scanner.results well-resolved,reproducible 2-de patterns of sgc7901/vcr and sgc7901 were established.silver staining was better when protein sample amount was low,overloaded protein will interfere resolution of the maps.gels stained with colloidal coomassie brilliant blue had more protein spots numbers and abundance without apparent trails when increased loading protein sample.conclusion two staining methods were influenced largely by the sum of protein samples,properly selection may be helpful for further study with proteomics in multidrug resistance of gastric cancer.

低甲氧基果胶的胶凝机理及防止预凝胶。。。他扎罗汀凝胶与克林霉素凝胶治疗痤疮。。。注射隆乳后经乳晕切口取出聚丙烯酰胺。。。目的分析利用蛋白质组学方法研究胃癌耐药相关蛋白质中双向电泳凝胶的染色显示。方法培养胃癌细胞sgc7901和长春新碱诱导的耐药胃癌细胞sgc7901/vcr,用双向凝胶电泳技术分离总蛋白,银染及胶体考马斯亮蓝染色,image scanner扫描仪扫描凝胶。结果获得了背景清晰、重复性好的双向凝胶电泳图谱,两种染色凝胶相比,硝酸银染色在样品少时显示更佳,过量则影响图像质量,而胶体考马斯亮蓝染色在上样量增加时的凝胶蛋白质点数目及丰度均增加,并无明显拖尾。结论两种显色方法受样品量影响较大,恰当选用有利于通过蛋白质组学研究胃癌耐药机制工作的进一步开展。

The study on themechanisums has been a hotspot of biology and radiobiology.

值得关注的是,自从国外学者提出电离辐射旁效应概念以来,许多研究者证实旁效应的存在,对&照射细胞核才会引起细胞致命的损伤&&只有受照射细胞才受到损伤&等某些传统观念提出了有力的挑战,辐射诱导的基因不稳定性、细胞质受到照射所致的突变及旁效应等在辐射致癌中同样具有重要作用,并得到放射生物学界的确认。

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In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国,慢性酒精中毒,肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题,你可以随时联系我。

Very pretty, but the airport looks more fascinating The other party wisecracked.

很漂亮,不过停机坪更迷人。那人俏皮地答道。