诱导的

Combining SSH and cDNA microarray to screen of up-regulated ESTs in the water stress Erianthus arundinaceus based on the analysis of up-regulated ESTs,a full-length cDNA sequence was cloned from sugarcane through Rapid Amplification of cDNA Ends method,and the gene expression character was analyzed by real time RT-PCR.

应用消减文库技术结合cDNA芯片技术筛选水分胁迫诱导的基因的EST序列，根据筛选到感兴趣的上调表达基因的EST序列，用RACE技术获得SSADH的全长cDNA序列，并通过实时荧光RT-PCR技术对该基因的表达特征进行分析。

The homeotic gene not only plays a role in the tegulation of cell ar- rangement in plant s organ, but also is the base of the floral meristem identity, inflores- cence meristem identity and floral organ identity.

植物由营养状态转入生殖状态是发育过程中的重要一步，其转变过程受遗传固子及环境的双重控制，光周期与温度是影响开花诱导的主要环境固子，而植物的同源异型基因参与植物器官内细胞排列方式形成的调拉过程，是拉制花分生组织特异性、花序分生组织特异性和花器官特异性的基础。

In this study, the amount of ethylene and its key biosynthesis genes expression, typical allelochemical hydroxamic acid concentration and the key regulation genes (BX1 and BX9), as well as the dynamic expression and time of relevant defense key genes of secondary substance metabolism processes were compared in ethephon treatments of different concentration and duration.

为探明外源乙烯利对玉米化学防御作用诱导的浓度和时间效应，本文采用化学和分子生物学相结合的方法，研究了不同浓度乙烯利处理玉米后，叶片乙烯释放量及其合成调控关键基因ACS和ACO表达的变化，典型化感物质丁布的含量及其关键调控基因BX1和BX9表达的变化，以及其他调控次生代谢物的关键基因表达的时间动态。

Focal ischemia induced by photochemically thrombotic distal middle cerebral artery occlusion model to adult rats results in necrosis at the infarct core and activation of complex signal pathways for cell death and cell survival in the penumbra. The effects of ghrelin on regional cerebral blood flow in penumbra areas used by a laser Doppler Flowmeter.

本实验室前期工作初步证明ghrelin可保护谷氨酸及脂多糖诱导的原代培养大鼠脑皮质神经元的损伤，也能减少线栓大脑中动脉引起的脑梗塞体积，说明ghrelin对脑缺血引起的神经元损伤具有一定的神经保护作用，有可能成为一个内源性神经保护多肽，具有重要的理论价值和实践意义，值得深入研究。

METHODS:The gene of human ET1 was synthesized according to the preferential codons of E. coli, cloned into the EcoRI and SalI sites of vector pThioHisA. The recombinant plasmid pThioHisA-ET1 was constructed , sequenced and transformed into E.coli TOP10. Induced and expressed fusion protein were identified and analysed by 12% SDS-PAGE and densitometry analyses. After the elution, denature and renature, the fusion protein Thioredoxin-ET1 was obtained by ProBondTM chromatogragraphy. The purity of Thioredoxin-ET1 was detected by HPLC. Inoculate Thioredoxin-ET1 once per mouse every 2 weeks in 25, 50 and 100μg separately on 3 groups for 4 times. 10 days after last inoculation, we obtained venipuncture blood.

根据人ET1的多肽序列合成ET1基因，将其插入到pThioHisA的EcoRI和 SalI位点，重组质粒pThioHisA-ET1进行酶切鉴定及序列测定验证后转化TOP10，IPTG诱导的重组菌经SDS-PAGE检测融合蛋白Thioredoxin-ET1的表达量；表达的融合蛋白用ProBond亲合层析纯化并经HPLC鉴测其纯度；每只小鼠按25、50、100ug/次剂量的Thioredoxin-ET1每两周免疫一次，共4次，最后一次免疫10d后制备抗血清，经Western blot和ELISA检测证明Thioredoxin-ET1融合蛋白具有ET1免疫反应原性。

It is suggested that mechanisms protecting epidermis of squamata species from UVB damage may involve in (1) Physical defensive mechanism: the death epidermal layer limited UVB transmitting to the viable cells;(2) Biochemical defensive mechanism: up-regulated CAT may protect skin from reactive oxygen species which may induced by UVB. Moreover, the lipid and proteins in the β-, meso- and α-layer may absorb incident UVB and formed damaged products that were limited in the original site;(3) Cellular defensive mechanism: quick initiating of proliferation of the germinal cells and forming of the new epidermis and shedding out the damaged epidermis shortened the damage process and restricted the damage area.

1物理性防御：角质层和中层阻挡绝大部分UVB透过，最大限度地减轻生发层的损伤；(2)生化性防御：通过上调CAT活性以减轻LVB诱导的活性氧对表皮的损伤，同时角质层蛋白质和中层脂类可吸收UVB，且形成的有害产物被局限于原位；(3)细胞性防御：通过迅速启动再生修复过程形成新的表皮，及时蜕去积累了脂类过氧化产物、受损蛋白质和晒斑细胞等的受损表皮，有效防止损伤的持续和蔓延。

The results suggested that vascular remodeling was the most prominent in LCA subjected to low shear stress accompanied by hypertension, which is regulated through the changing expression of p-Akt, p-p38, Rho GDIα. Tributyrin could affect the flow mediated carotid remodeling by both increasing apoptosis and decreasing proliferation of VSMCs through PI-3K/Akt, bcl-2, p53, p27. Tributyrin could have potential roles in treatment of vascular diseases, such as artherosclerosis and vascular restenosis, etc.

结果提示，高血压与切应力协同作用对血管重建的影响最为显著，Akt、p38和Rho GDIα信号通路参与了高血压与低切应力诱导的血管重建过程；Tributyrin对血管重建的干预作用是通过影响PI-3K信号通路，调节凋亡相关蛋白bcl-2以及细胞周期调控蛋白p53和p27的表达水平而实现的；Tributyrin有可能成为一种在动脉粥样硬化、血管再狭窄等疾病防治中有潜在临床应用价值的药物。

To get more factors associated with erythropoiesis in K562 cells, modified differential display reverse transcription polymerase chain reaction method was applied to identify genes with differential expression in uninduced and hemin-induced K562 cells. Remarkable differential ESTs were firstly selected for cloning, sequencing and analyzed with bioinformatics methods.

为了进一步获得分化过程中相关基因表达的信息，我们应用改良的差异显示RT-PCR技术，分析K562细胞在Hemin诱导的红系分化前后的基因表达变化，获得红系分化相关基因的线索，进而确定在红系分化中起重要作用的基因，并研究它们的作用机制。

In human, SP110b is the closest homology protein of IPR1 (41% identity), and its gene localizes in region of human chromosome 2. IPR1 and SP110b are both interferon inducible proteins, and they may have the same function involved in immunity in mice and human.

SP110b是IPR1在人类身上同源性最高的蛋白质(有41%的相似度)，它的基因座落在人类第二条染色体的区域，IPR1和SP110b都是受干扰素诱导的蛋白质，两者在小鼠和人类的免疫机制上可能有相同的功能。

Low concentration ryanodine can mimic BDNF to induce growth cone attraction and high dose ryanodine gradient is repulsive to the growth cone.

ryanodine 可以模拟 BDNF 的吸引作用。为进一步研究 Rho GTPases 在轴突转向中的作用，我们将 Cdc42及 RhoA 的显性失活突变基因(dominant negative， DN)及组成性激活突变基因（ constitutively active， CA ）注射到爪蟾胚胎2-4细胞期的一个细胞中，使它们在胚胎神经元中表达，并观察这些突变基因对 BDNF ， LPA ， ryonodine 诱导的轴突转向的不同影响，从而推测 Rho GTPases 是否直接参与并介导轴突导向。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。