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With in situ DNA hybridization, the EBV genomes have been demonstrated in the labial glands of the Sjogren′s syndrome patients (16/28), moreover the elevated content of EBV DNA has been identified in those with more severe destruction of labial glands. It is concluded that EBV, vhich has a normal site of latency at salivary glands in a nonpathogenic state, may be reactivated in Sjogren′s syndrome patients and play a role in the pathogenesis of this disease.

另外,我们用原位杂交方法观察了EB病毒DNA在干燥综合征病人唇腺组织的存在状况,结果发现28例干燥综合征病人中16例为阳性,其中腺泡组织破坏严重者,EB病毒DNA的含量亦高,并且EB病毒主要分布于大量浸润的淋巴细胞中,其次位于腺泡上皮细胞内,少量见于腺泡腔中,以上结果提示,在干燥综合征病人EB病毒处于活跃的复制状态,干燥综合征病人靶器官受损与EB病毒的活跃复制及其诱导的免疫反应密切相关。

Open field test showed a low level of locomotion, rearing and grooming, which indicate a disruption of engagement in both a social and nonsocial nature and decreased explorational ability. These findings all fit the behaviors of depression. The increased adrenal weight and increased total number of CRF immunoreactive neurons in PVN of hypothalamus indicated the hyperactivity in HPA axis.

以上指标符合抑郁动物模型的标准;肾上腺重量增加和下丘脑室旁核CRF阳性神经元数目增加提示HPA活性升高;DST显示,预给地塞米松(dexamethasone,DEX)不能减少CUMS处理的大鼠下丘脑室旁核CRF阳性神经元数目,与之对应的是,预给DEX能够显著减少急性应激诱导的大鼠下丘脑室旁核神经元的增加,这提示DST的失抑制。

We also give all positive self-adjoint extensions ofsingular differential operators,and all positive self-adjoint operators generated by theproducts of differential expressions 〓,where l is an nth order differentialexpression.The result that each positive self-adjoint operator is not necessarily theform of operator product 〓.This answers an open problem proposed by theauthors recently.

我们也给出了奇型微分算子的所有正自伴扩张形式及乘积微分算式〓所诱导的所有正自伴算子形式,证明〓所诱出的正自伴算子不必须是由算子乘积〓为l所生成的算子)的形式,从而回答了作者新近提出的一个公开问题。

Objective The arteria carotis externa was ligated,and arteria carotis interna was kept opening The left carotid artery was exposed and placed over an ultrasonic flow probe.The brain flow volume was recorded with an ultrasonic volume meter.The pursiness time of mice was observed to evaluate the effect of Erigeron breviscapus pellets on cerebral ischemia.Methods Born's method was used to investigate effects of Erigeron breviscapus pellets on rabbit platelet aggregation induced by adenosine diphosphate,arachidonic acid,and platelet-acti-vating factor,in vitro or in vivo.Antithrombotic effects of Erigeron breviscapus pellets was monitored accord-ing to Peter's method.

目的 应用二道血流速度流量仪超声探头测定鼠颈总动脉以反映云南植物药业有限公司提供的灯盏花颗粒对脑血流量的影响;记录小鼠断头后张口喘气时间,观察灯盏花颗粒的抗脑缺血作用方法采用Born方法测定灯盏花颗粒在体内外对AA、ADP及PAF诱导的家兔血小板聚集功能的影响;运用电刺激大鼠颈动脉血栓形成方法,评价灯盏花颗粒对血栓形成的阻抑作用。

HISI treatment prolong the survival time of mice in toxic condition induced by sodium nitrite and duration of electrocardiograph in trachea closed mice .HISI could obviously improve the ischemic electrocardiogram T-wave induced by Pituitrin.ST segment drifts on electrocardiogram in acute myocardial ischemia rats induced by Isoprotereno were obviously opposed by administration of HISI. The myocardial infarction areas, releasement of lactic dehydrogenase 、 creatine phosphokinase significantly increased in myocardial ischemia dogs, HISI treatment prevented these effects .

实验结果表明:苦碟子氯化钠注射液能显著延长小鼠常压缺氧环境及亚硝酸中毒情况下的存活时间,延长小鼠气管夹闭后心电消失时间;可显著改善垂体后叶素诱发的大鼠急性心肌缺血心电图T波异常;明显对抗异丙肾上腺素诱导的急性心肌缺血大鼠心电图ST段的偏移;显著改善冠脉结扎所致急性心肌缺血犬的心外膜心电图缩小其梗死心肌面积,降低心肌耗氧量,减少心肌坏死导致的心肌酶如乳酸脱氢酶、肌酸磷酸激酶的漏出。

One positive bipolaron can be slipt into two positive polaron, and one positive polaron can be split into one a negative polaron and the other a positive bipolaron—where this photoinduced carrier fission does not need the aid of an external electric field, which is a typical symmetry breaking.

一个正的双极化子裂变成两个正的单极化子,以及一个正单极化子可以裂变成一个正的双极化子和一个负的单极化子。光诱导的载流子裂变不需要一个外电场的帮助,是一个典型的对称性破缺。

Methods] MTT assay was employed to test the lethal concentration 50 (IC50) of sensitive germline (SK-OV-3) and ovarian cancer cell subline induced by ADR (SK-OV-3/adr) treated by 4 drugs, resistance index and reverse multiple were calculated. The changes of drug content were observed by fluorescence microscope, the intracellular accumulation of ADR was evaluated by fluorospectrophotometry, the changes of mdr 1/P-gp resistance were observed respectively by RT-PCR and Western blot.

方法]MTT法检测药物对敏感细胞株(SK-OV-3)和诱导的耐药细胞株(SK-OV-3/adr)的半数致死浓度(IC50),计算耐药指数和加逆转剂后的逆转倍数,荧光显微镜观察不同时间细胞内的药物含量变化,荧光分光光度法测定细胞内ADR的含量,RT-PCR和Western blot法检测细胞耐药前后mdr1/P-gp的变化。

AIM: To isolate bioactive secondary metabolites from the fruits of Schisandra rebriflora Rehd. et. Wils and study their effects on HIV-1 infectivity. METHODS: Inhibition of syncytia formation, HIV-1 reverse transcriptase and protease, protection of HIV-1 infected cells, level of HIV-1 p24 antigen,blockage of fusion were detected. RESULTS: A lignan compound, named rubrifloralignan A, was first isolated as a natural product with modest inhibition effects on syncytium formation and HIV- 1 replication and could protect HIV-1 infected cells with weak inhibition effects on fusion and RT, but it showed no effects on the replication of HIV-1 chronic H9 and PR. CONCLUSION: Rubrifloralignan A is an efficient anti-HIV-1 product with effect on early stage of HIV-1 replication.

目的:从红花五味子果实中寻找活性代谢产物,研究其抗HIV—1活性和作用机制方法:通过合胞体抑制、HIV-1感染细胞保护、HIV—1p24抗原测定、融合阻断、逆转录酶和蛋白酶活性分析等实验,检测分离化合物的抗HIV—1活性并探讨其作用机制一结果:红花五味子甲素能够抑制病毒诱导的合胞体形成,保护病毒感染细胞,抑制病毒在细胞内的复制对HIV-1感染细胞与正常CD4^+细胞间的融合,逆转录酶活性有一定的抑制作用但是红花五味子甲素不抑制病毒在慢性感染细胞中的复制,也不抑制蛋白酶的活性、结论:首次从天然产物中分离得到红花五味子甲素,它对HIV—1病毒复制早期具有抑制作用

Through above Molecular Cloning and Characterization of four Novel cold-responsive genes and correlative regulators from Capsella bursa-pastoris, we validated that there are cold-responsive genes Cbcor15b、Cbcbf、Cbice53、 CbLos2 in Capsella bursa-pastoris.

通过以上荠菜中4条新的与冷胁迫相关的基因及其转录因子的克隆和特征功能分析,证明荠菜中存在Cbcor15b、Cbcbf、Cbice53、CbLos2等与冷胁迫相关的基因,且它们的表达都是受冷条件诱导的

It may imply that there are close relation between the enhancement of electric field in nearby particles and dipolar interaction.

基底上正电荷所诱导的金属纳米粒子的偶极的形成减弱了从金属纳米粒子到PATP分子的电荷转移,碘离子的共吸附使得金属纳米粒子表面的电荷重新分布。

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She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了,但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场,你等着挨绳子吧!

Several theories about reigniting and extinguishing of the arc have been refered.

本文综合考虑了几种电弧重燃和熄灭理论。