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The friable embryogenic callus with small grains and without proembryo formation was screened in the process of continuous subcultures for 4~6 times on the medium with low content of sucrose from embryogenic cultures induced from immature embryos in litchi (Litchi chinensis Soon.). The well-scattered embryogenic cell suspensions could be rapidly established from the friable embryogenic callus in 2 liquid initial MS media respectively supplemented with 2,4-D 2 mg/L or 2,4-D 2 mg/L, KT1 mg/L and AgNO3 5 mg/L shaking at 100~120 r/min within 10-14 days.

荔枝幼胚诱导的胚性培养物在低糖条件下连续继代4~6次左右,可筛选到颗粒细小、不含原胚的松散型胚性愈伤组织;以这种松散的胚性愈伤组织作为起始材料,在附加2,4-D 2mg/L或2,4-D 2mg/L、KT1 mg/L、AgNO3 5mg/L的MS液体启动培养基上振荡培养(100~120 r/min)10~14 d,即可建立起分散性良好的胚性悬浮细胞系。

Methods Porcine coronary SMCs were incubated with serum-free medium containing different concentrations of simvastatin. Infiltration of 3H-TdR into SMC DNA was measured as an index of cell proliferation. Simvastatin's effect on migration was tested with Sarkar's technique.

采用体外猪冠状动脉SMC培养技术,以3H-TdR的参入量表示冠状动脉的SMC DNA合成情况,应用划线方法测定冠状动脉SMC的迁移距离,观察辛伐他汀对OX-LDL诱导的冠状动脉SMC增殖和迁移的影响。

Among the most critical experiments carried out by Monod and his colleagues was that which showed that enzyme induction resulted in the synthesis of an new protein and not the activation of some preexisting protein.

Monod和他的同事所做的最重要实验表明了酶诱导的结果是新蛋白质的合成而不是已有蛋白质的激活。

Therefore, TH-regulated metamorphosis, mediated by TR, is an ancestral feature of all chordates.

因此,由甲状腺素诱导的变态过程受到了甲状腺素受体的调节,这种变态过程是所有脊索动物的祖先特征,这种调节网络的保守性也支持变态在脊索动物谱系中是具有同源性的。

It has been shown that both SA and JA are in wounding signal transduction pathways. It has been suggested that cytokinins and small GTP_binding proteins might be the switch of JA and SA signaling pathways.

水杨酸可抑制茉莉酸类的合成及其所诱导的蛋白基因的表达;茉莉酸能阻止病原侵染后所产生的水杨酸的增加。

It is evidents that "Wenjing" a mixture shows certain inhibitory effect on the production of TNF-α and IL-1β in synovial cells, which may be one of the mechanism of the therapeutic effect on rheumatoid arthritis.

提示问荆合剂对Ⅱ型胶原诱导的大鼠关节炎具有一定抑制作用,可通过抑制关节滑膜分泌的炎性细胞原子TNF-α、IL-β的水平,从而减轻关节滑膜的炎症,可能是其治疗类风湿关节炎的作用机制之一。

Methods Fe2+VitCinduced lipid peroxidation damage models were established by rat liver microsomes extraction. The expressions of malondialdehyde and superoxide dismutase were tested.

通过大鼠肝微粒体的提取,制备Fe2+VitC系统诱导的脂质过氧化损伤模型,检测车前子多糖对丙二醛的抑制作用以及对超氧化物歧化酶活性的影响,观察车前子多糖的抗脂质过氧化作用。

Baicalin could inhibit the initial-period and mid-period activation of mouse T lymphocytes stimulated by Con A or PDB,which suggests that baicalin have the inhibitory effect on signaling pathway of T lymphocytes activation and be a potential effective immunoinhibitory agent.

结论黄芩苷能够明显抑制多克隆刺激剂Con A或PDB诱导的T细胞早期和中期活化,且呈明显的量效关系。提示黄芩苷在T细胞活化过程的信号转导中有抑制作用,从而可以通过抑制T淋巴细胞的活化,发挥免疫抑制作用,是一种潜在的免疫抑制剂。

Based on this result, it is concluded that the change of acetylation level at the loci of hsp, mediated by histone deacetylase inhibitors, exertsimportant functions in hsp gene transcription.3, After immunolabeling with anti acelated-lysine monoclonal antibody on the polytene chromosomes of heat shocked flies, fluorescence signals were detected at the hsp loci.

3,利用抗乙酰化赖氨酸的单克隆抗体对热激处理后的果蝇幼虫多线染色体进行的免疫荧光实验结果表明在热休克诱导的hsp70基因的表达中存在乙酞化修饰。

Through systematic study, it is found that the ion dose is another important parameter in Ion Beam Manipulation and it has significant influence on the strutrual phase transition. If the dose is precisely controlled, the structural phase transition could be traced under a quasi-continuous way. Consequently, many new phenomena have been discovered by Ion Beam Manipulation.

一、研究了离子束剂量在离子束操纵技术中的重要影响;研究表明离子束剂量可以精确地控制,使材料中的结构演变过程接近于连续地变化,因此可以对离子束诱导的结构相变进行准连续状态的跟踪,发现了许多新的现象。

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