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The addition reactions with phosphine anion andthe CsF promoted Strecker reactions were chose to evaluate the relationship of thesetwo chiral groups in the diastereoselective additions of the imino group.

通过亚磷酸负离子的加成反应和CsF促进下的Strecker反应研究了这两个手性基团的不对称诱导的匹配情况。

Gondii, were cloned by PCR respectively. The PCR products were digested by the corresponding enzymes and ligatd into the intermedial vectors. Finally, the inducible RNAi vector, pBSK-HSP70/5UTR-IntronC-HSP70/3UTR, with the HSP70 gene promotor as a promotor, the intron C sequence ofβ-tubulin gene as intervening sequence, 3UTR sequences of HSP70 gene as transcription stop signals, was constructed successfully, and the results of sequencing were correct. 3The construction of the inherited and inducible RNAi vector system of T. gondii: The fragment of SAG1/5UTR-eGFP-SAG1/3UTR in pBSK-SAG1/GFP vector was cloned into the vector of pBSK-HSP70/5UTR-IntronC-HSP70/3UTR to construct pBSK-GFP-Hairpin vector, then the fragment of GFP-Hairpin in pBSK-GFP-Hairpin vector was cloned into pHANA-0.5 vector.

弓形虫可诱导的反向重复序列RNAi载体的构建:设计引物,通过PCR分别扩增弓形虫HSP70基因5&UTR启动子序列(HSP70/5UTR)、HSP70基因3&UTR序列(HSP70/3UTR)及β-微管蛋白基因内含子C序列,通过酶切连接,构建以弓形虫热休克蛋白HSP70基因启动子进行驱动的,以β-微管蛋白基因内含子C序列作为间隔序列,以HSP70基因3UTR序列作为转录终止信号的反向重复序列RNAi载体pBSK-HSP70/5UTR-IntronC-HSP70/3UTR,序列测定结果正确。

Mechanistically, the dsRNA-induced gene actiation requires the Argonaute 2 (Ago2) protein and is associated with a loss of lysine-9 methylation on histone 3 at dsRNA-target sites.

在分子构成上,dsRNA诱导的基因激活需要有Ago2蛋白,同时还伴随有dsRNA打靶部位的组蛋白3上的赖氨酸-9甲基化的缺失。

In experimental animals, exposure to a common herbicide, rotenone, induces features of Parkinsonism; mechanistically, rotenone-induced destruction of dopaminergic neurons has been attributed to its inhibition of the activity of neuronal mitochondrial complex Ⅰ.

例如,Greenamyre和他的同事在2000年报道了经大鼠颈静脉慢性灌流鱼藤酮复制了帕金森病的主要特征,包括选择性黑质纹状体多巴胺系统变性,Lewy小体样细胞浆包涵体形成和一些行为变化,例如运动减少和僵直等。从发病机制上考虑,鱼藤酮诱导的多巴胺神经通路损伤被认为是由鱼藤酮抑制线粒体复合体Ⅰ活性引起的。

AIM: To examine the role of matrix metalloproteinases expressed by the tartrate-resistant acid phosphatase-positive mononuclear and multinucleated cells in articular cartilage damage.

目的:观察在胶原诱导的C57BL/6小鼠类风湿性关节炎模型中,基质金属蛋白酶-2、9在耐酒石酸盐酸性磷酸酶阳性的单个核及多核细胞中的表达及其在关节软骨损伤中的作用。

FIZZ1 had no effect on the expression of scavenger receptor CD36 in cultured macrophages. FIZZ1 can promote the development of AS by the up-regulation of the expression of SR-A and the phagocytose of fatty substance in VSMCs.

FIZZ1不影响体外培养的巨噬细胞清道夫受体CD36的表达,FIZZ1能促进ox-LDL诱导的体外培养的平滑肌细胞SR-A表达,从而可能加速平滑肌细胞吞噬脂质,促进动脉粥样硬化进展。

This review focuses on our current understanding of chemotherapy-induced nausea and vomiting and the status of pharmacologic interventions for their prevention and treatment.

这篇综述的话题针对当前对于化疗诱导的恶心和呕吐的理解,以及用药物对其进行预防和治疗的状况。

After 3 days treatment with RA, the cells began to show phenotypical changes, assuming a neuronal-like morphology with contemporaneous formation of dendritic-like structures.

诱导的d1、3、7,用相差显微镜观察细胞形态的变化;用免疫细胞化学染色、RT—PCR等方法检测p27^Kip1、p21Cip1及其相关的cdk2、cyclinE的变化。

The black spots ocurred in the fore wings of male were under photoperiodic control.

雄虫前翅黑斑的出现是由光周期控制的,只有在短日照下诱导的滞育蛹,滞育解除后羽化出来的雄虫才会出现黑斑。

Objective To investigate adenoviral vector mediated exogenous gene expression in mouse lun gs and the effect of mIFN-γ transgene expression on allergen-induced pulmonar y eosinophil infiltration in a murine asthmatic model.

目的研究重组复制缺陷腺病毒在小鼠肺内气道上皮细胞中的转基因表达水平及其介导小鼠γ-干扰素的转基因表达对过敏原诱导的小鼠哮喘模型的预防性治疗作用。

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She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了,但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场,你等着挨绳子吧!

Several theories about reigniting and extinguishing of the arc have been refered.

本文综合考虑了几种电弧重燃和熄灭理论。