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The pcDNA〓-apoE〓 plasmid was transfected to SK-N-SH Neuroblastoma cell by liposome. It could be screened by G〓. The immunohistochemical assay was shown that neuroblastoma cell transfected by pcDNA〓-apoE〓 expressed the recombinant apoE protein. The intracellular expressed recombinant protein could inhibit the death of neuroblastoma cell induced by beta amyloidal peptides 25-35 fragment.

用pcDNA〓—apoE〓真核表达载体与脂质体共转染神经成纤维胶质瘤细胞,用G〓选择压力筛选,细胞免疫荧光检测表明,pcDNA〓—apoE〓转染的神经成纤维胶质瘤细胞表达了apoE〓重组蛋白,这种内源性的apoE〓重组蛋白对25μM Aβ25—35多肽诱导的神经成纤维胶质瘤细胞的死亡具有拮抗作用。

RT-PCR amplification of ginseng β-amyrin synthase gene was successfully performed based on the total RNAs extracted from ginseng hairy roots induced by Agrobacterium rhizogenes A4 using a modified guanidine isothiocyanate-method.

以发根农杆菌A4菌株诱导的人参发根为材料,用改良的异硫氰酸胍法提取总RNA,得到了纯度好的完整的总RNA。

Methods The experimental arrhythmia models were induced with barium chloride (BaCl2),uabaina and aconitine in rats and cobayaes.The incidence rate and mortality rate of arrhythmia,the emergence time and the duration of arrhythmia induced with BaCl2 were recorded in rats pretreated with CASI (in dosages of 25,50, and 100 mg·kg-1 sublingual vein injection).At the same time,the dosages of uabaina and aconitine were observed when the ventricular premature contraction,ventricular tachycardia,ventricular fibrillation and cardiac asystole were appeard in rats and cobayaes pretreated with CASI (in dosages of 25,50, and 100 mg·kg-1 sublingual vein injection).

制备氯化钡(BaCl2)、哇巴因及乌头碱诱导的大鼠及豚鼠实验性心律失常模型,CASI按25、50、100 mg·kg-1分为3个剂量组,舌下静脉给药1次,不给CASI作为对照组,记录大鼠给BaCl2后心律失常的发生率、出现时间、持续时间及动物的死亡率;观察豚鼠及大鼠出现室性早搏、室性心动过速、心室颤动及心脏停搏时哇巴因及乌头碱的用量。

Doetschman TC, Eistetter H, Kemler R, et al. The in vitro development of blastocyst-derived embryonic stem cell lines: formation of visceral yolk sac, blood islands and myocardium [J]. J Embryol Exp Morph, 1985,87:27~45.[2] Robbins J, Doetschman T, Jones WK, et al. Embryonic stem cell as a model for cardiogenesis[J]. Trends Cardiovasc Med, 1992,2:44~50.[3] Moreadith RW, Radford NB.

诱导的心肌细胞的判定除常规指标外,我们还用了心脏特异性引物β-MHC对不同收缩时间的心肌细胞总RNA进行RT-PCR扩增和RNA斑点杂交,发现该心肌细胞不仅具有正常心肌细胞的特性,而且在分化早期就高度地表达心脏特异性基因。

The value of creating a laser-induced chorioretinal venous anastomosis to treat RVO may bypass the occluded vein、relieve the venous obstruction、restore retinal circulation、 benefit retinal function and improve visual acuity.

激光诱导的脉络膜视网膜静脉吻合治疗RVO,可以缓解阻塞静脉的血液回流障碍,帮助恢复视网膜循环,改善视网膜功能,提高视力,是一种较安全的、可行的治疗RVO一种新方法。

Using the intracellular recording technique for monitoring the excitatory postsynaptic potentials of CA1 pyramidal neuron and delivering an activator of protein kinase C and/or inhibitors of PKC and 〓/calmodulin-dependent protein kinase Ⅱ into postsynaptic cell in hippocampal slices, we studied the role of postsynaptic PKC and CaMKⅡ in long-term potentiation induced by high frequency stimulation at the CA1 synapses made by Schaffer collateral/commissural and perforant pathway afferents.

用电生理学方法在大鼠海马脑片CA1区锥体细胞记录Schaffer侧枝和穿通通路到CA1神经元突触的兴奋性突触后电位,通过送蛋白激酶抑制剂和激活剂进入突触后细胞,我们研究了突触后蛋白激酶C和依赖钙/钙调蛋白的Ⅱ型蛋白激酶在高频刺激诱导的长时程增强产生和维持中的作用。

They are expressed at high levels constitutively; however, the expression of some members of the HSP family is induced by stress-causing conditions such as heat shock, glucose deprivation, etc.

它们大都是高水平的组成型表达,然而HSP家族的一些成员的表达是在应激条件下被诱导的,比如热休克,去除葡萄糖等。

In order to investigate the effect of tumor necrosis factor α on the activation of astrocyte, the oligodeoxynucleotide was used to inhibit the production of TNF α from astrocytes stimulated by Coriaria Lactone.

为进一步探讨 TNF-α在星形胶质细胞激活中的作用,用 TNF-α反义寡核苷酸抑制由马桑内酯(coriaria lac-tone,CL )诱导的星形胶质细胞 TNF-α的产生,观察 NFκBp6 5核转位和 IκBα降解的变化。

Activities of phenylalanine ammonia - lyase, cinnamic acid 4 - hydroxylase and diterpene cyclase in rice plants are induced when their leaves are irradiated with UV light for 12 hours and 24 hours.

紫外光处理水稻化感材料后12 h和24 h,被诱导的苯丙氨酸氨基裂合酶、肉桂酸-4-羟化酶(CA4H)和二萜环化酶的酶活力比紫外光处理的无化感作用水稻中的要高2-4倍。

When the readily available homochiral alcohols were used as substrates, highly optically active products 4-vinyl-2-oxazolidinones were easily obtained.

在这个基础上,我们利用手性底物出发进行了手性诱导的反应研究,在两价钯的催化下能够以很好的收率得到光学纯的产物。

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