诱导的

The callus induction best culture medium is WPM ZT 1.5mg/L (above inculture medium sugar is 2.0%, agar-agar is 0.8%, the PH value is 5.6), its induction leads reaches as high as 88.0%.

愈伤组织诱导的最佳培养基为WPM ZT 1.5mg/L（以上培养基中均加入蔗糖2.0%，琼脂0.8%，PH值为5.6），其诱导率高达88.0%。

Along with the fluconazole solution density rise,the experimental two kind of strain various glucose density is higher,showe d the glucose consumption are less,takes the logarithmof the medicine de nsity,discovered the logarithm the medicine density and each glucose den sity presents the linear relations;Carries on the analysis comparison to under the fluconazole function two kind of strain linear relations,disc overed the relations of the two strains has the nonuniformity.3 Compare the fluconazole induction reaiatance SC5314 strain and sens itive strain compares,its difference gene expression mainly concentrates in:The code proteinase body and the protein hydroltyic enzyme gene,in the code sugar fat metabolism process is connected the protein gene,the cell cycle correlation gene,the duplication and the translation adjustme nt correlation gene,the stress response correlation gene,the line plast ochondria correlation gene,the cell wall function related gene.4 Candida albicans SC5314 induction resiatance strain was processed b y Xianglian solution,its expression change gene mainly is:Code stress re sponse family protein gene,biomembrane relevant gene,a code proteinase body gene race,code cell cycle related protein gene,duplication and tra nslation adjustment related protein gene.5 The clinical reaiatance strain Candida albicans was processed by Xi anglian solution,its expression change gene mainly is:Codes the hot sho ck protein gene,the serine/threonine protein activating enzyme gene,the proteinase body family gene,the regulation copies and translates the ge ne.

随着氟康唑药液的浓度上升，试验的两种菌株各孔葡萄糖浓度越高，说明葡萄糖消耗越少，经过药物浓度取对数后进行分析，发现取对数后的药物浓度和每孔中葡萄糖浓度者呈现线性关系；对氟康唑作用下的两种菌株的线性关系进行分析比较，发现对两种菌株作用具有不一致性。3氟康唑诱导的耐药SC5314菌株与诱导前的敏感株相比，其差异基因表达主要集中在：编码蛋白酶体及蛋白水解酶的基因，编码糖脂代谢过程中相关蛋白的基因，细胞周期相关基因，转录及翻译调节相关基因，应激反应相关基因，线粒体相关基因，细胞壁功能相关基因。4白念珠菌SC5314诱导耐药株经香莲外洗液作用后，其表达变化的基因主要是：编码应激反应家族蛋白的基因，生物膜相关性基因，编码蛋白酶体基因一族，编码细胞周期相关蛋白基因，转录及翻译调节的相关蛋白基因。5白念珠菌临床耐药菌株经香莲外洗液作用后，其表达变化的基因主要是：编码热休克蛋白基因，丝氨酸/苏氨酸蛋白激酶基因，蛋白酶体家族基因，调控转录及翻译基因。

The expression of spinach glycolate oxidase in pichia pastoris appeared to be induced by methanol, the molecular weight of glycolate oxidase was about 39.8kDa by SDS-PAGE and consisted with the data of literature.

甲醇诱导重组菌表达，并对诱导的条件进行优化，确定甲醇最适剂量为0.5%，最佳表达时间为72hr，最适温度为28℃，最佳pH为5.5，最佳表达菌体密度A600为0.8。

Results In the pyramidal neurons voltage-clamped at -60 mV, GABA evoked a transmembrane inward current and the peak amplitude of the current increased sigmoidally with increasing doses of GABA applied. Co-application of lidocaine resulted in a decline of IGABA and the IGABA was 4.05mmol/L.10mmol/L lidocaine resulted in right shift of concentration-response curve arid not only EC50 changing from 7.72mmol/L to 17.2mmol/L but also the maximal current unable to reach the top level.

结果 将钳制电位固定在－60mV，GABA以剂量依赖性方式诱导出内向电流；利多卡因明显抑制GABA诱导的Cl电流I(下标 GABA，50%抑制浓度(IC50)为4.05mmol/L，10mm/L可使GABA浓度效应曲线明显右移，50%有效浓度(EQ50)由7.72mmol/L增加到17.2mmol/L，且最大电流明显降低。

The content of CYP0 tented to decrease in the formation process of rat primary carcinoma of liver induced by DAB.DAB could not induce the activity of ANDM,but it could induce to increase the active level of GST.

DAB诱导的原发性大鼠肝癌形成过程中肝脏的 CYP 0的含量有下降的趋势，DAB不能诱导 ANDM活性，但能诱导增强 GST的活性水平。

Objective To study the differential display of promyletic leukemia cell lines induced and non-induced by all-trans retinoid acid.

摘 要］目的对经全反维甲酸诱导和未诱导的HL-60细胞差异表达基因进行初探。

RESULTS ① TLSFJM could induce apoptosis of PHA plus IL-2-activated mouse thymo cytes;② TLSFJM could induce apoptosis of alloantigen activated human pe ripheral blood mononuclear cells and hold the cell cycle at G1 phase;③ TLSFJM neither influenced the CD25 expression nor induced apoptosis of PMA activated human PBMC .

结果 TLSFJM 在体外可诱导PHA/IL-2活化的小鼠胸腺细胞的凋亡。 TLSFJM 在体外可诱导alloantigen活化的人外周血单个核细胞的凋亡，且把细胞周期阻遏在G1期。 TLSFJM 对PMA诱导的人PBMC活化抗原CD25的表达无明显影响，也不引起凋亡。

Cell lines were co-tranfected with bi-plasmids and cultured at 1% O〓 condition, luciferase assay demonstrated that all of the expression systems with different domains could be hypoxia-activated. GAL-CAD achieved more than 100-fold induction in A549 cell and more than 40-fold in HepG2; expression level of the system markedly increased after addition of glycin arm, almost 10-fold enhancement in HepG2 cell. Because basic expression also increased, the induction fold by hypoxia decreased.

双质粒共转染不同细胞株后，在1％O〓环境下培养24h，荧光素酶活性检测结果提示含不同结构域的表达系统均有受缺氧激活的能力，其中GAL-CAD在A549中获得100倍以上的诱导，在HepG2中获得40倍以上的诱导；加入甘氨酸臂后该表达系统的转录活性大为增强，在HepG2细胞中增强了10倍以上，但是由于基础表达也有所增加，受缺氧诱导的倍数下降。

The effects of phenolic acid compounds in vegetables and fruits on metabolicactivation of BaP in rats,as well as 〓 and TCA induced lipid peroxidation inmouse and Salmonella typhimurium TA104 cells were investigated using DNAadducts as a biomarker to assess DNA damage induced by xenobiotic carcinogens.BaP was used to study the genotoxic carcinogenesis,while 〓 and TCA were usedto study the non-genotoxic carcinogenesis.The possible protection mechanisms ofphenolic acid compounds on DNA damage were studied.

本研究根据化学致癌过程的遗传毒性和非遗传毒性机理，以DNA加合物作为机体接触外来化学物而导致DNA损伤的生物学标志物，通过观察酚酸类化合物对大鼠体内BaP活化代谢以及〓和TCA诱导小鼠肝微粒体脂质过氧化的影响，及酚酸类化合物对BaP和TCA诱导的沙门氏菌TA100和TA104形成DNA加合物的影响，探讨蔬菜、水果中酚酸类化合物对外来化学物诱导的DNA损伤的保护机制。

Although the first intron of hsp90β contains several typical HSEs which can bind PHA activited HSF trimers in other hsps, it has no effects of PHA induced expression of hsp90β.

PHA通过5＇上游远端的序列诱导hsp90β的表达，hsp90β的第一内含子虽然含有数个典型的HSE，却不参与PHA诱导的应答；启动子虽然可以介导高水平的基础转录，却对PHA的诱导不敏感。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

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如果有任何问题，你可以随时联系我。