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Results hPDLP wasisolated from human periodontium and most of the cells retained their fibroblastic spindle shape. hPDLP can be in-duced into osteoblast-like cells and adipocyte-like cells, and calcium deposition and lipid droplets were detected perspectively.

结果 第1代hPDLP的CD146和STRO-1阳性率分别是27.20%±3.98%和4.23%±4.08%;经矿化诱导可以形成矿化结节,有钙盐沉积;经成脂诱导可见特异性脂滴形成,特异性转录因子过氧化物酶体激活物增生受体2(PPARγ2)和脂蛋白脂酶表达上调。

In 5 μM. pseudolaric acid B-treated HeLa cells, caspase-3 proenzyme decreased followed by the degradation of caspase-3 substrates, ICAD (inhibitor of caspase dependent DNase) and PARP poly-(ADP-ribose polymerase.

土槿皮乙酸5μM作用于HeLa细胞后,caspase-3的酶原被降解,caspase-3底物ICAD(inhibitor of caspase activated DNase)和PARPpoly-(ADP-ribosepolymerase降解,caspase-3的抑制剂可部分抑制土槿皮乙酸诱导的细胞死亡,表明土槿皮乙酸诱导HeLa细胞凋亡时激活了caspase-3。

The reactant in an induced reaction that has an increased rate of reaction in the presence of the ''.

受体,受容体在一个诱导反应中的反应物,因诱导剂的存在而有增加的反应速度

CONCLIUSION: Human myoblast could be induced to neuron-like cells treated with ciliary neurotrophic factor and Salvia miltiorrhiza injection. The p44/p42 kinase participates in redifferentiation of myoblasts by phosphorylation.

经睫状神经因子预诱导后逐渐去分化的成肌细胞,在丹参注射液的诱导下能够成功分化为表达神经元特异蛋白标记物的神经元样细胞,p44/p42激酶通过磷酸化参与了成肌细胞的再分化过程。

According to analysis of root exudates by HPLC, aluminum induced roots of stylo to secret citrate and its secretion increased with Al concentration treated (0, 10, 20, 50 umol L-1) and the period of exposure to Al (6, 12, 18, 24 h), which indicated that Al-induced secretion of citrate is an important mechanism for stylo to resist the toxicity of aluminum.

采用高效液相色谱法对根系分泌物的分析发现,铝诱导柱花草根系分泌柠檬酸,并且其分泌量随铝处理浓度0、10、20、50 μmol L~(-1和时间(6、12、18、24 h)的增加而增加,表明铝诱导根系分泌柠檬酸是柱花草抵御铝毒害的重要机制。

In order to understand the adaptation of the microorganisms under the extreme cold environment conditions, the physiological characteristics of the 5 bacterial isolates from the Muztag Am ice core under the different temperatures were investigated. The csp gene was also amplified by PCR technique and the specific primer targeted for the cold shock protein. The observation results showed that there were three phenotypes of microorganisms: psychrophiles growing from 0~23°С(the optimum temperature at 15°C), psychrotolerant with growth range at 0~40°C (the optimum temperature at 23°С), and the thermophile with growth range at 0~45°C(the optimum temperature at 30°C).

为了解冰川微生物的生理生长特征及其在极端寒冷环境条件下的冷适应特性,从慕士塔格冰芯分离菌株中选取了5个代表菌株,分别对这些菌株的生长温度和酸度范围进行了观测,并利用微生物冷诱导蛋白的保守氨基酸序列,设计了1对冷诱导基因csp的特异引物,并对其进行了PCR扩增。

Key words:Dendrobium candidum; Axenic nodal segments; In vitro propagation; Concentration of hormone; Organic susbstance

方法将铁皮石斛试管苗茎节接种于不同NAA浓度的MS培养基上诱导腋芽萌发,并在含不同激素配比和有机添加物的培养基上继代和诱导生根。

Two asymmetric photochemical reactions were investigated in such medium: photochemical reduction of phenyl cyclohexyl ketone and photoelectrocyclization of tropolone methyl ether.

虽然固相光化学反应的手性诱导取得了可喜的进展,但对底物和诱导剂的要求比较苛刻,使其适用范围非常有限。

In conclusion, the hepatotoxicity of clivorine is caused by the hepatotoxic pyrrolic metabolites formed in vivo. CYP3A involved in the formation of pyrrolic metabolites and plays a key role in metabolism-induced hepatotoxicity of clivorine.

Clivorine的肝毒性是其在体内形成的肝毒性吡咯代谢物所致,CYP3A参与了肝毒性吡咯代谢物的形成并在clivorine的代谢致毒方面发挥了关键性的作用,因此CYP3A的诱导剂和抑制剂可以影响肝毒性吡咯代谢物的形成,从而影响clivorine的肝毒性。

In this study, We used longer DD primers in combination with a two-step PCR protocol to overcome these two problems in term of modified methods of Martin and Pardee.K562 erythroleukemia cell differentiation induced by HEMIN or PMA for 36 hours was used as erythriod or megakaryocytic differentiation model in vitro.Total RNAs were extracted from induced and uninduced K562 cells and were applied to mRNA differential display analysis.

为解决此问题,我们参照Martin和Pardee的改进方案[3],建立了一种使用长引物,进行二步PCR的改进DDRT-PCR方法,以氯高铁血红素和肉豆蔻佛波酯诱导K562细胞36小时作为体外红细胞和巨核细胞分化模型,取诱导前后的细胞总RNA进行mRNA差异显示分析。

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