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The apxⅢA gene of Actinobacillus pleuropneumonie was amplified by PCR. The amplified DNA fragment 3 466bp was cloned into pMD18-T. After R.E. analysis and sequencing, the apxⅢA gene in pMD18-T was ligated into pBluescripⅡSK, the recombinant expression plasmid pET-28b/apxⅢA was constructed and analysed with R.E., the protein of apxⅢA gene expressed in E.coli BL21 was detected by Western blotting.

因胸膜肺炎放线杆菌的致病性主要是由毒素决定的,故参照猪胸膜肺炎放线杆血清2型菌株的序列(GenBankL1 2 1 4 5)设计了一对特异性引物,用PCR的方法扩增apxⅢA基因并得到了长 3 466bp的片段,然后将其克隆到pMD 1 8T中,经酶切鉴定和序列分析表明克隆是成功的;再将apxⅢA插入到原核表达载体pET 2 8b后,转化BL2 1 (DE3),在IPTG诱导下获得高效表达,经Westernblotting检测证实表达产物有活性。

The results show that perfect anatase nanocrystalline TiO2 can be obtained by SGRTSE method and the photocatalytic activity of TiO2 can be improved by doping and surface treatment. In the experimental system, the addings of proper salts are beneficial to the photocatalytic degradation of DBSNa. The pH value of solution, adding amount of TiO2, flow rate of air, and initial concentration are the main influence factors for the photocatalytic degradation of methyl orange, and the apparent reaction order is pseudo-first-order, which could be described by Langmuir- Hinshelwood equation.

结果表明:sol-gel法结合室温溶剂蒸发诱导可制备出晶型良好的锐钛型纳米TiO_2;过渡元素和稀土元素杂原子掺杂,贵金属掺杂和表面酸化处理是有效提高TiO_2光催化活性的途径;试验体系中,在DBSNa的降解过程中加入适量的盐,可以大大加速降解效果;光催化降解水中有机物,溶液pH值,TiO_2投加量,曝气速率,被降解物起始浓度等是影响降解效果的重要因素;对起始浓度较低的有机物光催化降解过程可利用简化的Langmuir-Hinshelwood动力学方程进行描述,反应级数为拟一级。

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推荐网络例句

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称;三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起,称之为挽歌体诗人或者史诗诗人,他们被称为诗人,似乎只是因为遵守格律写作,而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。

This is not paper type of business,it's people business,with such huge money involved.

这不是纸上谈兵式的交易,这是人与人的业务,而且涉及金额巨大。