诱导物

objective the aim of this study is to investigate the expression and the distribution of the nerve growth factor during the period of neural tube development of human embryo.method early development of neural tube was studied in human embryos about 35 gestational days by using immunocytochemical abc technique.result there were ngf immuno-positive substances in the cytoplasm and nuclei of neuroepithelial cells in the ventricular zones of neural tube.in the intermediate zone of neural tube,ngf immunoreactivity was detected in the nuclei of some neurons,or the processes of other neurons which contained no ngf-immunoreactive substances in their nuclei;the expression pattern of ngf in the marginal zone of neural tube was similar to that of the intermediate zone.the density of ngf-immunorecative particles was higher on the rostrum side of neural tube than on the caudal side.the ngf immuno-positive cells were also observed among the somites of embryo under the neural tube.conclusion these results suggest that ngf was an important signal molecule to induce neural tube differentiation,and that ngf may play a significant role in regulation of the biological function of neurons in developing neural tube.

目的 研究捷安肽素的抗真菌作用机理。方法采用形态学方法和同位素标记法。显微形态观察经捷安肽素处理后的供试真菌的形态学变化。进一步采用14c同位素标记的特异底物&尿苷二磷酸-（14c）-葡萄糖&示踪，研究捷安肽素对真菌(1，3)-β-d-葡聚糖合成酶活性反应的影响。结果研究神经生长因子在早期人胚神经管发育过程中的定位表达。方法采用免疫细胞化学 abc法染色，研究35天人胚的发育情况。结果在人胚神经管的室管带中，神经元的细胞质和细胞核ngf免疫反应阳性；在中间带，一部分神经元的细胞核ngf免疫反应阳性，另外一部分神经元的细胞核ngf免疫反应阴性，而其突起ngf免疫反应阳性；在边缘带ngf的表达与中间带相似。在神经管的头侧ngf阳性反应较强，神经管的尾侧ngf阳性反应较弱。结论 ngf在人胚神经管免疫反应阳性，表明ngf可能是诱导神经管分化发育的重要信号分子，提示ngf可能在人胚神经管的发育中具有十分重要的作用。神经生长因子；人胚；神经管；发育

The results led to the conclusion that induction and repression were under the control of specific proteins, called repressors, which were coded by regulatory genes.

得出了诱导和阻遏是由被称为阻遏物的特殊蛋白质控制的结论，阻遏蛋白是由调节基因编码的。

The fluorescence of DNP can be quenched by RNA and its constitutive bases; at the same time, significant fine stucture of the excimer band can be induced by these quenchers.

RNA及其组成碱基除了能猝灭DNP的荧光以外，还诱导其激基缔合物谱带产生精细结构。

Then, it was condensed with-valine methyl ester to form a chiral imine, which was reacted with allyl zinc bromide in the presence of CeCl3·7H2O to afford the corresponding chiral homoallylic amine with high optical activity.

-N-Boc-3-苯基-2-氨基丙醛与-缬氨基酸甲酯缩合生成手性亚胺，在七水合三氯化铈（CeC13·7H2O）催化以及底物的手性诱导作用下，该手性亚胺与烯丙基溴化锌经不对称加成反应合成了高光活性的单烯丙基化手性胺。

The highest PLB multiplication rate was obtained on the media containing 2 mg/L of zeatin or BA, about 70% of the explants generating 16 or more PLBs in a month. The PLBs proliferated from the above experiment were subcultured one by one on the MS basal salt media with the organic compound made of yam bean or potato homogenate for shoot formation investigation. After 60 days, PLBs, from the zeatin treatment, subculture on the medium containing yam bean had the highest shoot formation rate.

进一步将上述试验所得之PLB单颗接种於含有80g/L豆薯或马薯的基本培养基中，比较有机添加物对PLB之芽体形成之影响，结果显示自2mg/Lzeatin处诱导所得之PLB，接种在豆薯培养基中有最高之芽体形成；而自IBA处中所诱得之PLB在豆薯及马薯培养基中皆有高比之芽体形成。

Devise a pair of primer to put out the section of pgE which code the dense antigen episode, clone into pET-32a, name the masculine plasmid with pET-32pgE. Transform the BL21 strain with pET-32pgE, choose several single bacterium drops at random and contaminate 2X YT. vibrate at 37癈 over night. Contaminate another tube with the above culture to the final concentration of 2%, continue to culture to OD6oo0.6, add IPTG to the final concentration of Immol/L. inducing 6 hours. Analyse the expression with SDS-PAGE and Western-blot, the result is that the expression has actually happened and the product has immunoreactivity.

设计一对引物从gE全基因克隆载体pgE中调出抗原决定簇密集编码区，克隆进PET-32a表达载体，双酶切和PCR鉴定出阳性者，命名为PET-32pgE，转化BL21菌株，挑取单菌落在加有氨苄的2×YT培养基中37℃过夜长至饱和，按2％体积转种另一管，培养至OD_(600)为0.6，加IPTG至终浓度1mmol／l，诱导4～6小时，SDS-PAGE和Westernblot分析，证明确实表达并具有免疫反应性。

Sliver nanoplates were prepared through a visible light induced reduction process by a reaction between sodium citrate and silver nitrate in an aqueous solvent at room temperature. UV-Vis spectra were employed to monitor the growth of the silver nanoplates.

在水相体系中以柠檬酸钠和硝酸银为反应物，利用可见光诱导的还原过程在室温下制备盘状的银纳米粒子，通过紫外-可见光谱监控了不同反应条件下银纳米粒子的生长过程及体系的稳定性。

Based on the results, a substrate-induced fusion of the vesicles is proposed and get support from a fluorescence resonance energy transfer assay.

对此我们提出了底物诱导的囊泡相互融合的机理，并用荧光共振能量转移的方法证明了此机理。

Refining grain behavior of the heat affected zone of E36 high strength low alloy steel was studied, when E36 steel was welded by three wires submerged arc welding with 110 kJ/cm large heat input.

研究了焊接热输入为110kJ/cm三丝埋弧自动焊E36高强度低合金钢热影响区的晶粒细化行为；用自动图像分析仪测量了焊接热影响区诱导晶内铁素体形核夹杂物的大小、分布；用透射电镜观察了具有自细化行为晶内铁素体的形貌。

Refining grain behavior of the heat affected zone of E36 high strength low alloy steel was studied, when E36 steel was welded by three wires submerged arc welding with 110 kJ/cm large heat input .

大热输入焊接时，氧硫复合物诱导热影响区的晶内铁素体形核，并促进晶内铁素体的感生形核，晶内铁素体及其感生晶内铁素体使热影响区粗晶区的晶粒细化，确保焊接热影响区粗晶区的强度与韧性不降低。

The reasons of iron ions content overproof in grade Ⅱ desalting water system,such as variation water quality,contamination of regenerant , operation adjustment of pretreatment system and switching operation of bed were discussed.

对二级脱盐水系统中铁离子含量超标的原因，如来水水质发生波动、再生剂受到污染、预处理系统操作调整、床体运行切换等进行了论述。

You were hired to drum up new business, so go and do it.

公司雇你招徕新业务，你就做你的事好了。