诱导物

OBJECTIVE: To investigate the biocompatibility of poly (DL-lactide-coglycolide). Copolymer ratio of lactide: glycolide was 85:15 or 50:50and evaluate its effect on nerve guidance.

目的：对共聚比为(85：15)及(50：50)的聚乳酸-聚羟基乙酸共聚物材料的生物相容性及神经再生诱导作用进行对比观测。

We aim to screen and identify the key potential trans-factors during hemoglobin switch. We firstly analyzed differential expression of mRNAs in erythroid induction cultures of CD34+ cells derived from normal umbilical cord blood, adult bone marrow, and bone marrow of a heterocellular hereditary persistence of fetal hemoglobin patient. We identified ZF (HCF-binding transcription factor, Zhangfei) and SH3GLB1(SH3-domain GRB2-like endophilin B1) that had differential expression in the above three cultures. Furthermore, we confirmed the different expression of the above two genes by quantitative real-time PCR.

为鉴别影响珠蛋白基因表达和开关的新的重要调节因子，我们首先分析了人脐带血、正常成人骨髓和异细胞型胎儿血红蛋白持续存在综合症患者骨髓细胞红系诱导培养物中基因表达的差异，发现ZF(HCF-binding transcription factor，Zhangfei)和SH3GLB1（SH3-domain GRB2-like endophilin B1）在这三种不同来源的红细胞内具有显著的表达差异，并通过实时定量PCR方法验证了差异的真实性。

When the readily available homochiral alcohols were used as substrates, highly optically active products 4-vinyl-2-oxazolidinones were easily obtained.

在这个基础上，我们利用手性底物出发进行了手性诱导的反应研究，在两价钯的催化下能够以很好的收率得到光学纯的产物。

When they combined with antibodies and deposited on the lamella lucida of BMZ, the classical pathway of complement was activated, and induced production of many kinds of mediators of inflammation, resulting in the disorganization of half desmosome and forming hydroa under the epidermis.

抗原抗体反应形成的免疫复合物沉积在基底膜带透明板部位，后者激活补体经典途径，诱导多种炎症介质的产生，导致半桥粒结构破坏并形成表皮下水疱。

In this study, the amount of ethylene and its key biosynthesis genes expression, typical allelochemical hydroxamic acid concentration and the key regulation genes (BX1 and BX9), as well as the dynamic expression and time of relevant defense key genes of secondary substance metabolism processes were compared in ethephon treatments of different concentration and duration.

为探明外源乙烯利对玉米化学防御作用诱导的浓度和时间效应，本文采用化学和分子生物学相结合的方法，研究了不同浓度乙烯利处理玉米后，叶片乙烯释放量及其合成调控关键基因ACS和ACO表达的变化，典型化感物质丁布的含量及其关键调控基因BX1和BX9表达的变化，以及其他调控次生代谢物的关键基因表达的时间动态。

The N gene ORF was then subcloned into pET-30a vector and the recombinant plasmid was transformed into E.coli BL21 (DE3) and induced with IPTG. The protein expression was determined by SDS-PAGE. The expressed protein had a molecular weight of 54.4 Ku that existed as inclusion body. Thin-layer scanning showed that the expression product accounted for 30.5% of the total bacterial proteins. The recombinant protein possessed native biological activity and could react with anti-PEDV hyperimmune serum in Western blot.

以阳性质粒为模板，用分别含有BamH Ⅰ和Xho Ⅰ酶切位点的上、下游引物扩增得到ORF，其PCR产物经BamH Ⅰ和Xho Ⅰ双酶切后定向克隆到pET-30a载体，构建的重组质粒命名为pET-30a-PN；将pET-30a-PN转化到大肠杆菌BL21 (DF3)中，在IPTG诱导下进行表达；SDS-PAGE结果表明表达出与预期大小相符的约54.4 Ku的重组蛋白，重组蛋白以包涵体形式存在；薄层扫描结果表明表达产物占菌体总蛋白的30.5%；Western blot分析表明表达的重组蛋白能与抗PEDV高免血清反应，说明该重组蛋白具有免疫学活性。

Gondii, were cloned by PCR respectively. The PCR products were digested by the corresponding enzymes and ligatd into the intermedial vectors. Finally, the inducible RNAi vector, pBSK-HSP70/5UTR-IntronC-HSP70/3UTR, with the HSP70 gene promotor as a promotor, the intron C sequence ofβ-tubulin gene as intervening sequence, 3UTR sequences of HSP70 gene as transcription stop signals, was constructed successfully, and the results of sequencing were correct. 3The construction of the inherited and inducible RNAi vector system of T. gondii: The fragment of SAG1/5UTR-eGFP-SAG1/3UTR in pBSK-SAG1/GFP vector was cloned into the vector of pBSK-HSP70/5UTR-IntronC-HSP70/3UTR to construct pBSK-GFP-Hairpin vector, then the fragment of GFP-Hairpin in pBSK-GFP-Hairpin vector was cloned into pHANA-0.5 vector.

弓形虫可诱导的反向重复序列RNAi载体的构建：设计引物，通过PCR分别扩增弓形虫HSP70基因5&UTR启动子序列(HSP70／5UTR)、HSP70基因3&UTR序列(HSP70／3UTR)及β-微管蛋白基因内含子C序列，通过酶切连接，构建以弓形虫热休克蛋白HSP70基因启动子进行驱动的，以β-微管蛋白基因内含子C序列作为间隔序列，以HSP70基因3UTR序列作为转录终止信号的反向重复序列RNAi载体pBSK-HSP70／5UTR-IntronC-HSP70／3UTR，序列测定结果正确。

The present calculations demonstrate that O－ associates quickly with C2H4 to form ion-induced dipole complex, which can subsequently isomerize and decompose to various products, corresponding to molecular anions dissociation and associative electron detachment pathways, respectively.

计算结果表明， O－与C2H4经碰撞快速复合形成离子诱导偶极络合物中间体，然后经历异构化、解离生成各种产物，分别对应分子离子异构解离与复合电子剥离反应通道。

The present calculations demonstrate that O(superscript -) associates quickly with C2H4 to form ion-induced dipole complex, which can subsequently isomerize and decompose to various products, corresponding to molecular anions dissociation and associative electron detachment pathways, respectively.

计算结果表明，O与C2H4经碰撞决速复合形成离子诱导偶极络合物中间体，然后经历异构化、解离生成各种产物，分别对应分子离子异构解离与复合电子剥离反应通道。

In oil system, zymolytes show stonger inhibition to the autooxidation of grease and linolic acid and delay the induction period.

在油质体系中，酶解物能很好的抑制猪油和亚油酸的自氧化的发生，延长亚油酸自氧化的诱导期。

The reasons of iron ions content overproof in grade Ⅱ desalting water system,such as variation water quality,contamination of regenerant , operation adjustment of pretreatment system and switching operation of bed were discussed.

对二级脱盐水系统中铁离子含量超标的原因，如来水水质发生波动、再生剂受到污染、预处理系统操作调整、床体运行切换等进行了论述。

You were hired to drum up new business, so go and do it.

公司雇你招徕新业务，你就做你的事好了。