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The ORF of profilin of Caryota mitis pollen was amplified by RT-PCR using specific primers and cloned into the expression vector pET-28a. The recombinant Caryota mitis pollen profilin was expressed in Escherichia coli BL21 (DE3). The recombinant protein was purified by affinity chromatography using Ni(superscript 2+) coupled sepharose.

然后设计带有酶切位点的特异性引物,采用RT-PCR获得整个短穗鱼尾葵花粉profilin的开放阅读框,将其与pET28a载体连接并转化大肠杆菌BL21(DE3)进行诱导表达,通过Ni(上标 2+)亲和层析柱对重组蛋白进行纯化,采用Western-blot检测其IgE结合活性。

Therefore, the axially chiral monometallic or bimetallic complexes could be formed by the chelation-induction concept upon complexing process of axially achiral ligands.

因此通过金属配位诱导,在配位基团上手性元素的影响下得到轴手性金属配合物。

Immunocytochemical staining was conducted for the neural stem cells, neuron and neuroglia specific cell surface marker such as nestin, MAP-2, NSE and GFAP.

免疫细胞化学方法检测诱导前后神经前体细胞、神经元和神经胶质细胞特异标记物如nestin,MAP-2,NSE和GFAP的表达。

Based on studies about current development of Methods of separating meiofauna, a new method is advanced in this paper. A suit of device was designed to Separate meiofauna from benthic sediments sample automatically, accurately and efficiently, which based on the technology of Laser-induced Fluorescence Detection, and used other technologies, such as noctilucence, electronics, biophysics, computer, mechanism, and so on. The scheme of macrocosm, the design of light route, the hardware and software design of the photoelectricity detection system and machine control system, the method of signal processing are introduced, especially the hardware and software design and the method of signal processing.

论文分析了目前国内外所使用的小型底栖生物分离方法现状,并以此为基础,自主开发了一套基于激光诱导荧光技术,并且集荧光化学技术、电子物理技术、光电测量技术、计算机技术及机械技术为一体的小型底栖生物标本自动分离系统,实现了从沉积物样品中自动分离小型底栖生物标本,从而大幅度提高室内小型底栖生物分样的工作效率和精度的目的,并以此完成了大洋协会"十五"攻关项目:"小型底栖生物标本自动分离系统研制"。

Our result shows that for block copolymers in nonselective solvents,we can induce the micellization through the hydrogen bonding complexation betweenone of the block and its complementary polymer.

我们的结果表明,嵌段共聚物在非选择性溶剂中,能通过某一嵌段与另外一种高分子之间的氢键作用导致的络合诱导胶束结构的形成。

This phenomenon is caused by the kinetic competition between nucleation process and growth process.

基于这一发现,我们改变剪切诱导亚稳区中的升温速率,在共混物中发现两类不同的变化。

The results also suggested that a better capacity for osmoregulation in S. przewalskii might account for its greater capacity for freezing tolerance than S. chinensis .

祁连圆柏的相对含水量和自由水含量均低于圆柏,3种有机渗透调节物含量均高于圆柏,表明祁连圆柏在抗冷冻性诱导中具有更广泛的适应性策略。

With temperature increasing, granular outshoot prefer to grow along with vertical direction of surface of BN core, as well as the wrapped leaf-like shape form from granular ones. And the productions always kept complete morphology of sphere, which was due to the uniform growth of fluffy film on the BN core. Wrapped leaf-like structure of BN piece in fluffy film favored increasement of measured surface area and elimination of oxygen element.

凝并在球核表面的中间产物受到初始BN球核在空间上的诱导作用,随着温度的升高,突出物倾向沿着垂直于球面的方向向外生长,形貌由普通的粒状转变为卷叶状;绒膜的厚度均匀一致,产品得以保持规整的球状形貌,绒膜中卷叶状的BN结构利于氧杂元素的脱除,同时增加了比表面积。

The effect of supplementing ammonium sulfate, peptone and yeast extract on the rljGH production was further investigated.

进一步考察了在诱导阶段添加硫酸铵、蛋白胨和酵母抽提物的影响。

In the work, the laser-behavior under exposure of Ar〓 laser was discussed. The kinetic process of photochemical reaction, intermediate, sensitive process of diazophthoquinon system were studied by laser fluorescence spectrum and laser induced fluorescence attenuation. The nonlinear relation between photodecomposition rate of the system and laser power and concentration of solution was investigated in detail.

论文也进行了感光组成物感激光性能的研究:利用激光荧光光谱及激光诱导荧光衰减法实现了实时探测邻叠氮萘醌型光敏剂感激光光化学过程、中间体的产生与结构以及增感作用的研究,该方法在国内外文献中未见有报道。

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推荐网络例句

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称;三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起,称之为挽歌体诗人或者史诗诗人,他们被称为诗人,似乎只是因为遵守格律写作,而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。

This is not paper type of business,it's people business,with such huge money involved.

这不是纸上谈兵式的交易,这是人与人的业务,而且涉及金额巨大。