诱导地

PartⅡTransform the yeast, screen the excellent expression yeast strain, induce the yeast to express the fusion protein and depurate the target protein. The recombination plasmid was transformed into the yeast GS115 successfully by means of electrotransformation. The excellent yeast strain was got by G418 screening. We achived the yeast supernatant including target protein induced by methanol.

第二部分：酵母的转化、蛋白高表达菌株的筛选、重组蛋白的诱导表达及纯化实验利用电转化的方法，成功地将重组质粒pPIC9k-PTD-hA20导入毕赤酵母GS115，并通过G418浓度梯度筛选得到了蛋白高表达菌株，并对蛋白高表达菌株进行甲醇诱导表达，得到了含有目的蛋白的酵母上清液。

Three kinds of plant expressing vector pC1304- CamV35S+ Cbcor15b, pC1304-cold-induced promotor+ Cbcor15b*, pC1304- CamV35S+ Cbcbf for transforming dicotyledon plants was constructed successfully by substituting the report gene of pC1304 with the coding sequence of our destination gene. The recombinant vector was introduced into Agrobacterium tumefaciens with freeze-thaw method. The leaf discs of tobacco plants infected by A. tumefaciens were selected with hygromycin. The regenerated plants were tested by PCR.

在成功的构建pC1304- CamV35S+Cbcor15b，pC1304-冷诱导启动子+Cbcor15b*，pC1304- CamV35S+ Cbcbf三种植物表达载体之后，采用冻融法成功地将重组质粒转化到了根癌农杆菌菌株EHA105中，采用农杆菌介导的烟草叶盘转化法，将荠菜Cbcor15b，Cbcor15b*，Cbcbf，转入模式植物烟草中，在诱导生根后对转基因苗进行了PCR检测。

Yeast elicitor repeatedly added on days 15, 17 and 19 at the dose of 106 mg total sugar/1 medium improved PeGs accumulation further, and the final PeGs content and production reached 31.3 mg/g DW and 317.8 mg/1, which were 195%and 235% that of the control, respectively.

研究表明酵母诱导子的添加同时促进了细胞生长和PeGs合成，在培养第15、17和19天以106mg总糖／l的浓度三次加入酵母诱导子极大地刺激了PeGs的合成，其含量和产量在21天分别达31.3mg／g DW和317.8 mg／l，为对照培养的195％和235％。

A calculating method for finding the induced geodesic torsion was developed by using differential geometry and other mathematics tools.

为了分析线接触共轭齿面的诱导短程挠率，并清楚地诠释有关公式及计算推导的过程，运用微分几何、向量代数、线性代数等数学工具，寻找了一种计算诱导短程挠率的方法。

In recent years, some researches proved that TCM could induced MSCs differentiated into neuron cells and osteoblasts effectively and the inductivity was high.

近年来，有研究证明中药能有效地诱导MSCs分化为神经元细胞和成骨细胞，并且诱导率高。

These mutations greatly changed the level of protein synthesis, lipogenesis and JAK2 phosphorylation stimulated by GH, although they did not affect the expression of the GHR and ligand binding.

研究结果表明豚鼠GHR中的一些氨基酸残基的取代并不影响生长激素通过它而诱导的细胞事件，而另外一些氨基酸残基的取代，例如168位的酪氨酸和332位的丝氨酸分别用在其它种属GHR中高度保守的精氨酸和酪氨酸的取代后，则明显地影响着豚鼠GHR介导的GH诱导的生理效应。

As an ideal prime immunization vaccine,the DNA vaccine has the advantages of high purity,continuous expression of antigens with low dose, repeatable immunization and inducement of memory CTL with high titer.Similarly, recombinant virus can not only highly express the target antigen,simulate the inducement process of virus infection on body immunity,and induce various cytokines,but also be beneficial to the interaction of high-affinity antibodies with T cell receptors,and the generation of Th1 reaction and CTL reaction.

DNA疫苗抗原纯度高，持续表达低剂量的抗原，可多次重复免疫以及诱导高效价记忆性细胞毒性T淋巴细胞反应，是理想的用于初次免疫的疫苗；重组病毒能高效表达目的抗原，模拟病毒感染诱导机体免疫的天然过程，能诱发多种细胞因子，而且有利于高亲和性抗体和T细胞受体的选择，可以有效地产生Th1型和CTL反应。

Results Powder,the decocted ex-tracts and the non-heating extracts of lumbricus prolonged the occlusion time of the common carotid artery induced by the electrical stimulation in the dose-dependent way.

结果 地龙磨粉、水煎液和免加热提取物均可剂量依赖性地延长电刺激诱导的颈总动脉堵塞时间，地龙磨粉和水煎液抗动脉血栓作用强度相当，地龙免加热提取物抗动脉血栓作用强于同剂量的地龙磨粉或水煎液。

The hydrodynamic performance and the calculation of induced velocity along flow on the constant point after foil shows that if the effects of tip vortex and decay of vortex panel strength be considered, the accuracy of calculation can be increased. Velocity peak value in propeller wake current and distribution of induced velocity after three-dimensional short foil else can be calculated accurately.

通过对三维短翼的水动力性能和翼后给定点沿流向的诱导速度计算表明：采用考虑翼梢涡影响和涡的衰减影响的计算模型，可以提高三维短翼的水动力性能计算精度；可以准确地计算出螺旋桨尾流场的速度峰值；可以较好地计算出三维短翼翼后的诱导速度分布。

Western blot analysis with the antiserum ATP95 revealed a significant increase of protein amounts of the V-H〓-ATPase subunits B and c of S. slasa under 100 and 400 mmol/L NaCl treatment, which gave another evidence for a salt-induced coordinate up-regulation of V-H〓-ATPase subunits at transcription and translation levels. The coordinated salt-induced increase of subunit B, H and c of V-H〓-ATPase from S. salsa at transcription and translation levels indicated an increase of V-H〓-ATPase holoenzyme amounts, which might be the reason for the increase of V-H〓-ATPase activity of S. salsa under salt stress.

400 mmol／L NaCl处理盐地碱蓬植株分离其叶片液泡膜微囊进行Western-blot分析发现盐胁迫明显诱导了盐地碱蓬液泡膜H〓-ATPase B、c亚基蛋白的表达，证明盐胁迫下，盐地碱蓬液泡膜H〓-ATPase各亚基在转录、翻译水平存在协同表达。100、400mmol／L NaCl处理亦明显增加了盐地碱蓬叶片液泡膜微囊H〓-ATPase活性，表明盐胁迫下，盐地碱蓬液泡膜H〓-ATPase各亚基在转录、翻译水平的协同表达增加了H〓-ATPase全酶的数量，进而增大了H〓-ATPase的活性。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。