诱导地

At passage 3, fetal MSCs were induced in 100 nmol/L dexamethasone, 10 mmol/L β-glycerophosphoric acid and 50 mg/L vitamin C for 14 days.

取第3代胎儿间充质干细胞，以100 nmol/L地塞米松、10 mmol/L β-甘油磷酸和50 mg/L维生素C持续诱导14 d。

BMSCs were isolated from adult rats by modified bone marrow culture method. BMSCs were inoculated in basic medium containing 10%fetal bovine serum, high glucose DMEM, 100 U/L penicillin, 100 U/L streptomycin and 2 mmol/L glutamine in the control group. BMSCs were inoculated in conditioned medium (basic medium, 10 mmol/L β-glycerophosphoric sodium, 10-7 mol/L dexamethasone, 50 mg/L vitamin C).

通过改良的骨髓培养法分离成年大鼠骨髓间充质干细胞，对照组以基础培养液(10%胎牛血清+高糖DMEM+100U/L青霉素+100 U/L链霉素+2 mmol/L谷氨酰胺)培养，实验组用条件培养液(基础培养液，10 mmol/Lβ-甘油磷酸纳，10-7 mol/L地塞米松，50 mg/L维生素C)进行诱导培养。

To further investigate their roles in cell proliferation apoptosis and differentiation, three human leukemia cell lines (K562, U937 and HL60) exposed to various concentration of hexamethylene bisacetamide, a potent inducer of differentiation of transformed cells both in vivo and in vitro, were used as an in vitro model for the study of cell proliferation, differentiation and apoptosis.

为了进一步研究cyclin A和p21在白血病细胞增殖，分化，凋亡中的作用，我们用在体内，外均有抗肿瘤作用的药物六亚甲基二乙酰胺在体外抑制白血病细胞株K562，U937和HL60增殖，诱导其分化，成功地建立了与药物干预浓度呈剂量-效应关系的，具有不同增殖，分化，凋亡状况的实验细胞模型。

The hydroxamic acid compounds can also inhibit histone deacetylase and are suitable for use in selectively inducing terminal differentiation, and arresting cell growth and/or apoptosis of neoplastic cells, thereby inhibiting proliferation of such cells.

所述的异羟肟酸化合物还可以抑制组蛋白脱乙酰基酶并且适合于选择性地诱导终末分化，以及阻止赘生性细胞的细胞生长和/或细胞程序死亡，由此抑制这些细胞的增殖。

Our results indicate that VacA disrupts the apical membrane-cytoskeletal interactions in gastric parietal cells,and thereby causes hypochlorhydria.

我们对VacA感染的细胞中Ezrin的水解现象进行研究，为全面地认识幽门螺旋杆菌诱导胃酸分泌减少的现象提供了一个全新的方位。

The result fights allergic experiment to show, dispel the wind stops land of can different level restrains urticant Ding allergy of akin and passive skin mixes the bandicoot that antiserum causes 2, the sex of small rat late hair of chloric benzene be caused by exceeds 2 nitro- of 4- quick the thymus index that reaction; still can increase small rat when dosage greatly and lienal index; stop urticant experiment shows, dispel the wind stops urticant Ding can reduce the time of fit of small rat itching with dextrose lead anhydride and itching duration apparently, can increase Guinea pig to be able to bear or endure those who suffer phosphoric acid histamine send urticant threshold.

结果抗过敏试验显示，祛风止痒酊能不同程度地抑制抗血清诱发的大鼠同种被动皮肤过敏和2，4-二硝基氯苯所致的小鼠迟发性超敏反应；大剂量时还可增加小鼠的胸腺指数及脾指数；止痒试验显示，祛风止痒酊能明显降低右旋糖酐诱导的小鼠搔痒发作次数及搔痒持续时间，并能增加豚鼠耐受磷酸组胺的致痒阈。

Results We obtained a lysogenic bacteriophage MZTP01with clear plaque and 1 mm diameter. Fragment D with 2362bp (Genebank No. AY639599) was obtained after the phage DNA hydrolyzed by HindⅢ/EcoRⅠ. Among fragment D, the pep gene with molecular weight of 47 kDa and length of 1101bp was cloned and expressed. Recombinant M15 (pQE30pep) was built and overexpressed in Escherichia coli with a 47kDa clear band. At the same place a clear band was observed by Western blot. Judging from the time course expression, we could conclude that PEP protein produced at 1 hour after induction and then increased gradually. PEP protein was mainly in the form of inclusion body in the recombinant and slowed the growth speed of host. Homologous comparison of PEP protein from phage MZTP01 with other PEPs from BLAST were that phage MZTP01 PEP protein had 100% homologe with that of Escherichia coli K12, and most of others took the similarity in the range between 37%~84%.

诱导获得的溶原性噬菌体MZTP01斑点清晰，直径约1mm，成斑时间12h；从噬菌体基因组DNA双酶切(HindⅢ/EcoRⅠ)片段中回收长度为2362bp的D片段(Genbank登录号： AY639599)，又从D片段中克隆了长度为1101bp、编码367aa、分子量为47kDa的pep基因，表达载体M15(pQE30pep)在大肠杆菌(Escherichia coli， E.coli)中表达获得了47kDa的清晰表达带，在1h 时开始产生蛋白并有逐步上升的趋势； Western blot 也在47kDa处得到一条清晰的条带；可溶性分析表明PEP蛋白在重组菌株中是以不可溶的包含体形式存在的，该蛋白的产生明显地抑制了宿主的生长速度；噬菌体PEP氨基酸序列之间的同源性比较表明，噬菌体MZTP01 PEP蛋白与来自E.coli K12噬菌体的PEP蛋白的同源性程度最大。

To induce callus tissues and establish suspension culture of Marchantia polymorpha L.

目的探计对地钱愈伤组织的诱导和悬浮细胞培养的条件。

Successful induction of the callus tissues and establishment of suspension culture of Marchantia polymorpha L. provide a new pathway for exploring the pharmaceutical moss to produce secondary metabolism and a new theory guidance for suspension culture of moss cell in a large scale.

结论地钱愈伤组织的诱导成功和悬浮细胞培养条件的建立，为探索药用苔藓植物次生代谢产物生产提供新的途径以及对苔藓植物细胞规模化培养提供新的理论指导。

Our study showed that about 3% mononucleated cells of human peripheral blood could be induced to osteoclast-like cells which have the ability of bone resorption and show TRAP-positive when coculture with UMR106 in the presence of 1,25 dihydroxyvitamin D3, macrophage-colony stimulating factor and dexamethasone.

在1，25_2D_3、地塞米松和M-CSF存在下，人外周血单个核细胞与UMR106细胞共培养，约3%的单个核细胞可被诱导成为具有骨吸收能力且抗酒石酸酸性磷酸酶染色阳性的破骨细胞样细胞。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。