诱导地

The plant expression vectors were constructed and two genes were transferred into A. thaliana and N. tabacum, antikanamycin plants were obtained.4. The trials of adventive bud regeneration directly and callus induction was conducted to select out the optimal explants and formula. The results revealed that double scale can produce 13~16 adventive buds per centimeter in MS medium added 10.0 mg/L 6-BA, 1.0 mg/ L NAA, 40 g/L sugar and 7.5 g/L agar. Scale, leaves, scape and different tissue of flower can be induced to produce callus in appropriate medium, but the filament with anther was the optimized explant.

构建了两个基因的pBI121和pCAMBIA1300植物表达载体，并通过农杆菌将上述基因转入烟草和拟南芥中，得到了卡那抗性的再生植株。4、以中国水仙不同部位外植体进行了不定芽直接分化和愈伤组织诱导分化试验，结果表明，双鳞片外植体在MS0+6-BA 10.0 mg/L+NAA 1.0 mg/L+蔗糖40 g/L+琼脂7.5 g/L培养基上可以大量地直接再生，再生芽数量平均可达13~16个/cm双鳞片，比传统的鳞茎盘之间分化提高30~50%；鳞片、叶片、花葶及花部不同组织在适宜的配方中均可不同程度地诱导出愈伤组织，但以带花药的花丝诱导出的愈伤组织最好。

Results TFC significantly inhibited mice ear swelling and rat palm swelling and rat cotton pellet granuloma. It also showed that the content of PGE2 and LTB4 from PMФ were inhibited by TFC in dose-dependent manner.

结果 TFC对二甲苯诱导的小鼠耳肿胀、角叉菜胶诱导的大鼠足爪肿胀、棉球诱导的大鼠肉芽肿具有抑制作用；TFC在0.05~50mn/L范围内能够浓度依赖性地抑制PMФ中PGE2和LTB4的产生。

From the research that drug expenditure affective facors modol according to the drug consuming of three cities,we find that the quantity and introduce new drug is as important facors as the price factor,so we can\'t consider the price policies only,but to igore controling drug consuming quantity.

研究中还发现如果没有人监督医生的诱导需求行为，病人因无法识别医生的诱导需求而不加选择地雇用医生，则无论病人给医生多高的工资，也无法控制医生诱导需求的行为。

Immune response research shows one of the antimicrobial peptide, Attacin, was upregulated expressed in the tsetse fat body after trypanosome infection. But the infection was not effectively cleared due to the late response or expression site discrepancy .

对采采蝇的免疫研究发现它在感染锥虫后会诱导表达一种抗菌蛋白，Attacin，但由于时间太晚以及表达部位的原因，并不能有效地清除体内的锥虫感染，而当人为地诱导表达Attacin后再感染锥虫则可以发现感染率显著下降。

Cells in the induction group were incubated in 1.5 mL of osteoinductive medium, supplemented with 10-8 mol/L dexamethasone, 10 mol/L β-glycerophosphoric acid, and 50 mg/L vitamin C.

选取第3代骨髓间充质干细胞和脂肪间充质干细胞，按5×107 L-1接种后各分为2组：诱导组加入含10-8 mol/L地塞米松、10 mol/L β-甘油磷酸、50 mg/L维生素C的成骨诱导培养基1.5 mL，未诱导组不加入成骨诱导培养基。

METHODS: The third passage of BMSCs and AMSCs at a density of 5×10^7 L^(-1) were divided into two groups. Cells in the induction group were incubated in 1.5 mL of osteoinductive medium, supplemented with 10^8 mol/L dexamethasone, 10 mol/L β-glycerophosphoric acid, and 50 mg/L vitamin C. Cells in the non-induction group were not treated with osteoinductive medium.

选取第3代骨髓间充质干细胞和脂肪间充质干细胞，按5×10^7L^(-1)接种后各分为2组：诱导组加入含10^8mol/L地塞米松、10mol/L β-甘油磷酸、50mg/L维生素C的成骨诱导培养基1.5mL，未诱导组不加入成骨诱导培养基。

Bone marrow mesenchymal stem cells could differentiate into osteoblasts, adipocytes and neural like cells with osteoblast inductor (β-sodium glycerophosphate, dexamethasone, vitamin C), lipoblast inductor (dexamethasone, 3-isobutyl-1-methylxanthine, bovine insulin, indometacin) and serum-free medium inductor (dimethyl sulphoxide, butylated hydroxyanisole) respectively. Osteoblast marker (alkaline phosphatase, osteocalcin mRNA, calcium node), adipocyte marker (lipid droplet, PPAR γ-2mRNA) and neural cell-like marker (nissl body, neuron specific enolase, neurofilament protein) were respectively determined by the immunohistochemical method, polymerase chain reaction and immunocytochemical method.

分别采用成骨细胞诱导剂（β-甘油磷酸钠，地塞米松，维生素C）、成脂肪细胞诱导液（地塞米松，甲基异丁酸黄嘌呤，牛胰岛素，吲哚美辛）及二甲基亚砜和羟基丁酸苯甲醚无血清培养基诱导剂干预细胞向成骨、脂肪、神经细胞分化，经免疫组织化学染色、PCR、免疫细胞染色方法检测成骨标志物（碱性磷酸酶、骨钙素mRNA、钙结节）、脂肪标志物（脂滴、PPARγ-2mRNA）、以及类神经标志物（尼克氏体、神经烯醇化酶、神经丝蛋白）。

Bone marrow-derived mesenchymal stem cells are capable of chondrogenesis, making them a possible source of cells for injectable cartilage tissue engineering. There exist different ideas on the ability of mesenchymal stem cells's chondrogenesis in monolayer culture. Because of this, the effect of adult rabbit's bone marrow-derived mesenchymal stem cells chondrogenesis in monolayer culture was studied. The mesenchymal stem cells was isolated from adult rabbit's bone marrow and monolayer cultured. TGF-β1, Vit-C and Dexamethasone were used. Immunohistochemistry analyses and histological staining of H-E, Methylaniline blue and Alcian blue were performed to identify the expression of collagen type Ⅱ and cartilage associated matrix. The results showed that the induced cells expressed and produced collagen type Ⅱ and cartilage associated matrix. This suggests that the differentiation of adult rabbit's marrow-derived mesenchymal stem cells into chondrocyte in monolayer culture is feasible and may be induced by TGF-β1, Vit-C and Dexamethasone.

骨髓基质干细胞的软骨分化潜能使其可能成为可注射组织工程化软骨研究的种子细胞，为探讨体外培养的骨髓基质干细胞在平面诱导条件下软骨分化的可行性，我们进行下列实验：获取并体外平面培养成体兔骨髓基质干细胞，应用TGF-β〓、Vit-C和地塞米松对其软骨分化诱导，诱导后的骨髓基质干细胞行细胞爬片组织学和Ⅱ型胶原免疫组化，结果证实，诱导后骨髓基质干细胞可分泌Ⅱ型胶原，组织学染色可见类似于软骨细胞，由此证明体外培养的骨髓基质干细胞在平面诱导条件下可以软骨分化，其软骨诱导因子为TGF-β〓、Vit-C和地塞米松。

The trial results show that with the disposal of induced caving roof by presplitting blasting and induced caving roof blasting, about 17.8 m in thickness of rock cushion is successfully formed in the inferior abandoned stope, which can bring down the harm of dashing blast of an explosion caused by roof collapse; apparent run-through flaws arise between presplitting blasting holes, and controllable caving is formed in the roof, abrupt collapse of large area roof is prevented, the productional safe surroundings of underground mining is improved; eventually, the resources' reclamation rate is improved from about 70% to 84.6%, and the dilution rate is abated from 25%40% to below 15%, so efficiently withdrawing is actualized.

试验结果表明：采用预裂爆破与诱导崩顶爆破的方法，通过对顶板的诱导崩落处理，成功地在下方采空区形成了厚度为17.8 m左右的岩石缓冲垫层，有效地降低顶板冒落带来的冲击气浪危害；在预裂孔间产生明显的贯通裂隙，从而在顶板上形成诱导可控崩落，防止顶板大面积的突然垮落，改善地下开采安全生产环境；最终资源的回收率由70%左右提高到84.6%，贫化率由25%~40%降低到15%以下，实现了资源的高效回收。

METHODS: Normal human BMSCs were isolated and cultured by the whole bone marrow method. Cells of the third passage at 1×108/L were incubated on coverslip in a 6-well plate, and randomly divided into 3 groups: osteogenic induction group, which was added with primary medium and osteogenic inducer, supplemented with 10-8 mol/L dexamethasone, 10 mmol/L β-phosphoglycerol and 50 mg/L vitamin C; leptin osteogenic induction group was added with 50 nmol/l leptin in addition to osteogenic inducer. Cells in the blank control group were only treated with LG-DMEM containing 10% fetal bovine serum.

采用全骨髓法体外分离培养人骨髓间充质干细胞，传至第3代以1×108 L-1密度接种至预置盖玻片的6孔培养板中，设立3组：成骨诱导组添加原代培养液后，再加入含10-8 mol/L地塞米松、10 mmol/L β-磷酸甘油、50 mg/L维生素C的成骨诱导培养基；成骨+瘦素诱导组添加成骨诱导培养基后，再加入50 nmol/L瘦素；空白对照组仅加入含体积分数为10%胎牛血清的LG-DMEM培养液。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。