诱导因素

OBJECTIVE: To establish an inducing system for differentiation of bone marrow mesenchymal stem cells into chondrecytes in two-dimensional culture in vivo, to analyze the best concentration of transforming growth favor beta-1 (TGF-β1) to induce the differentiation and the correlated influence factors for the directional differentiation, and to observe the changes of cell form and phenotype.

目的：建立二维培养条件下骨髓间充质干细胞分化为软骨细胞的诱导体系，分析转化生长因子β1作为诱导条件的最佳浓度，以及诱导骨髓间充质干细胞定向分化为软骨细胞的相关影响因素，观察细胞诱导后的细胞形态及表型变化。

OBJECTIVE: To establish an inducing system for differentiation of bone marrow mesenchymal stem cells into chondrecytes in two-dimensional culture in vivo, to analyze tbe best concentration of transforming growth favor beta-1 (TGF-β1) to induce tbe differentiation and the correlated influence factors for the directional differentiation, and to observe the changes of cell form and phenotype.

目的：建立二维培养条件下骨髓间充质干细胞分化为软骨细胞的诱导体系，分析转化生长因子β 1作为诱导条件的最佳浓度，以及诱导骨髓间充质干细胞定向分化为软骨细胞的相关影响因素，观察细胞诱导后的细胞形态及表型变化。

After this, the AL4 elicitor concentration and handling time were optimized for improving the essential oil yield in suspension cells of A.

此后以茅苍术悬浮细胞挥发油产量为指标，优化了内生真菌AL4诱导子添加浓度和诱导子诱导时间这二个因素。

In thispaper, the characteristics of protein gelation formation were introduced by physical and chemical aspects.

从物理和化学方面阐述了蛋白形成凝胶的特点，蛋白凝胶的内在因素和外部因素，主要介绍蛋白的热诱导、酸诱导和酶诱导凝胶的形成及其影响因素。

The results showed that 6-BA is a major factor for induction of axianry buds and roots, and that TDZ is the key to induction of tufty bud.

极差分析表明，6-BA是诱导荞麦茎段腋芽和根发育的主要因素，TDZ是诱导丛生芽的关键因素。

Using colchicine for the induction of polyploidy plants is one of the ways of artificial induction. Colchicine, root length and light were selected as the influencing factors to induce polyploidy Amorpha optimal with the root tip smeared, to explore the optimal induction conditions for polyploidy Amorpha optimal.

用秋水仙素对植物进行多倍体诱导是人工诱导的方法之一，为了探究诱导紫穗槐多倍体的最优化条件，选取秋水仙碱，根长以及光照情况3个影响因素，对紫穗槐根尖进行涂抹处理诱导多倍体。

Nerve stem cell can be differentiation into astroblast, oligodendrocyte and nerve cell induced by factors.

神经干细胞的增殖分化可受各种因素的影响，目前已知，许多细胞因子，生长因子，营养因子以及细胞外环境等因素的作用，可诱导神经干细胞向不同的终末细胞分化，若能在体外分离培养神经干细胞并在促分化因子的作用下，诱导神经干细胞向某一特定方向分化，将为干细胞的分化机制提供有力的实验依据。

Statistic analysis indicated that the main factor which influenced callus induction and the inducting rate of fasciculated shoots were explant, the optimal medium for callus induction was MS+1.0mg/L BA+1.0mg/L NAA+0.5mg/L 2,4-D+Leaves.

论文摘要：采用正交设计方法，研究了6-BA、NAA、2，4-D及外植体4种不同因素及其组合对钟花樱愈伤组织及丛生芽诱导率的影响，试验结果统计分析表明：外植体是诱导愈伤组织和诱导丛生芽的最主要因素。

ABSTRACT It is because that the excellent acceptors for maize transgenic engineering are insufficient in our country, especially in the southwest mountain areas of china and hereditary variation regularity for the two characters such as efficiency of embryonic callus induction and number of regenerating plant (these two characters were abbreviated to the nduction efficiency and number of regenerating in the following of the paper, respectively), which hint the maize culturing capacity, is not very clear. Therefore, aiming at picking out superior acceptors, we had made systematic researches on the two characters with combing traditional quantitative-character genetic analyzing methods such as single-factor genetic mating design, diallel crossing genetic design, genetic effect analyzing method and the modern molecular locating method such as QTLs'. The main results are followed.(1) 50 superior inbred lines and about 30 crosses in our country, especially in the southwest of China were used for identifying and selecting the superior genotypes in the above two investigated characters under the same culturing condition in 2000 and 2001. There was very significant difference among the genotypes in the both characters. But the two characters were not certainly related. Some genotypes such as 18-599 and 18-599 were very good in them. For some ones such as zong 31, induction was higher than 18-599 and 18-599 in the efficiency, but it was only 1/3 to the later in regenerating number. In some genotypes such as S37, R08, R15, P138, A318, induction efficiency was just about 3% and scarcely any regenerating plants were got. On the whole, hybrids acted better than inbreeds in the both characters.(2) Two kind of inbreeds were selected as parents of the Griffing's method 1. 18-599 and 18-599 and the inbred line zong 31 are one kind because they are not only superior in the characters of maize cross breeding, such as CA, resistance to disease and the important agricultural characters, but also excellent in transformation characters as the induction and regeneration.

针对我国、特别是西南山地所需玉米转基因工程育种优良受体极为匮乏和反应玉米幼胚培养能力的2个主要性状，即玉米幼胚胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数的遗传变异规律十分不清楚的实际情况，本研究从筛选玉米转基因工程所需要的优良受体入手，采用单因素遗传交配设计、双列杂交遗传交配设计、世代基因效应等传统数量性状分析方法，以及现代分子标记定位主效QTL分析方法，对玉米幼胚胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数等2个性状进行了较为系统的分析研究，取得以下主要研究结果：(1)于2000年和2001年通过对我国、特别是西南地区近50份优良自交系和近30个杂交组合，在相同培养条件下，对幼胚培养胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数等2个幼胚培养能力性状进行了筛选与鉴定，发现玉米不同基因型具有完全不同的幼胚培养胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数，但幼胚培养胚性愈伤组织诱导率与胚性愈伤组织绿苗发生数并不具有必然的相关关系，有的基因型，如自交系18-599和18-599在胚性愈伤组织诱导率和愈伤组织绿苗发生数等2个性状都表现相当优异；有的基因型，如自交系综31，仅幼胚培养胚性愈伤组织诱导率性状表现高于19-599和18-599，但在胚性愈伤组织绿苗发生数这一性状则与它们有相当大的差距，仅为19-599和18-599的1/3左右；有的基因型，如S37、R08、R15、P138、A318等玉米自交系不仅幼胚培养胚性愈伤组织诱导率很低，平均仅在3％左右，而且胚性愈伤组织绿苗发生数表现也很差，基本上没有分化成苗。

The summary results are below:1. GUS expression under the driving of the BjCHI1 promoter (-1060/+17) was essentially undetectable in the young seedlings under normal growth conditions. GUS activity was first detected in the stigma of young flowers, peaked in the young siliques, and decreased when the siliques became older. No GUS expression was found in the mature siliques, seeds or root.2. The BjCHI1 promoter (-1060/+17) was inducible by NaCl, PEG, wounding and MeJA treatments. High levels of GUS expression were detected in the transgenic tobacco and Arabidopsis plants after wounding, NaCl, PEG, and MeJA treatment, indicating that the BjCHI1 promoter responses to both biotic and abiotic stresses.3. RT-PCR analysis confirmed that the expression of the BjCHI1 gene in B. juncea was inducible by PEG and NaCl.4. The transcription start site was determined by 5′-RACE, and was located at the 17th nucleotide upstream of the translation initiation codon of the BjCHI1 gene.5. A -805/+17 promoter fragment was enough to response to wounding and MeJA induction, which was proved in transgenic tobacco and Arabidopsis plants. The 397 bp region between -805 and -409 of the BjCHI1 promoter contains a cis-acting element that is essential for the wounding and MeJA inducibility.6. The -695/-620 region was necessary but not sufficient to confer MeJA-responsive expression. A T/G-box locates in -353 play an important role in the expression of the BjCHI1 gene in response to MeJA treatment. The 76 bp region is coupled with the T/G-box to confer full MeJA-inducible transcription of the BjCHI1 gene.

主要结果如下：1、利用转基因拟南芥植株分析表明，正常生长条件下，BjCHI1启动子(-1060/+17)驱动GUS基因主要在花柱中表达，幼嫩的荚也有表达，并随着果荚的成熟而减弱，成熟的果荚、种子和根没有显示GUS活性。2、BjCHI1启动子(-1060/+17)能驱动GUS基因在转基因烟草和拟南芥中响应伤害的诱导，转基因拟南芥的分析还证明BjCHI1启动子也受MeJA、NaCl和PEG的诱导，证明BjCHI1启动子是一个伤害、MeJA、NaCl和PEG等生物和非生物因素诱导启动子。3、RT-PCR进一步证明芥菜中BjCHI1基因也受NaCl和PEG的诱导表达。4、5′-RACE法鉴定了BjCHI1启动子的转录起始位点，位于翻译起始位点ATG上游第17个碱基A.5、转基因烟草和拟南芥分析证明，-805/+17的启动子片段足以响应伤害和MeJA的诱导，-805和-409之间397 bp的启动子片段含有对伤害和MeJA诱导必要的元件。6、本明烟叶片瞬时表达系统分析证明，一段76 bp的序列(-695/-620)对BjCHI1启动子响应MeJA的诱导是必要的，但不足以响应MeJA的诱导，位于-353的T/G-box也参与MeJA的诱导。76 bp的序列(-695/-620)与T/G-box协同起作用，赋予BjCHI1启动子MeJA诱导性。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。