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First ,the project acquired complete technical route of tissue culture of Anthurium andraeanum and m...

首先,本项目完成了红掌组织培养的整套技术路线,完成了叶诱导愈伤组织、愈伤组织扩繁、芽丛增殖、生根和移栽等培养基培方的研究。

With the corm and scale of A. albus in Zigui County in Hubei Province as tested material, MS was used as the basic medium with different hormones to respectively match 10 kinds of media for induction, differentiation for tissue culture of A. albus The induced clumpy buds were cut into single plants and were put into the rooting medium of MS+NAA 0.1-0.5 mg/L and the effects of different hormone matching on callus formation, bud differentiation and rooting were studied.

方法]以湖北省秭归县的花魔芋球茎和鳞片作为外植体,以MS培养基为基本培养基,添加不同激素,分别组配成10种诱导培养基和分化培养基进行组织培养,然后将诱导的魔芋丛芽切成单株后接入MS+NAA0.1~0.5 mg/L生根培养基,研究不同激素配比对愈伤组织形成和芽分化以及生根的影响。

More precisely, we obtain an important relation between the mapping Ψ, which plays an important role in defining obstruction set, and the mapping on the cotangent bundle induced by the original diffeomorphism. We show that the only difference between them is a nonlinear factor.

在§3.2中我们得到了在定义阻碍集这一概念中起着关键作用的Ψ映射与原来映射在其余切丛上所诱导映射之间仅仅只相差一个非线性因子这一重要关系。

And then adding NAA at low concentration in MS was used to give rise to roots. We also found that lower level of hormone could control effectively browninng and vitrifaction during the culture and G1 n (6mg/L) and AgNOj (2mg/L) supplemented in shoot induction media could improve the shoot information rates apparently (about90%).The whole period of plant regeneration from leaflets of peanut could be divided as five steps: germination - shoot induction -shoot elongation-rooting- tranplant.

用1/2MS培养基萌发花生种子,9-10d后,从无菌花生苗上切取幼嫩叶片中部为外植体。2500Lux光照和27±1℃条件下,在诱芽培养基(MS+BA3mg/L+NAA0.8mg/L+AgNO_32mg/L十Gln 6mg/L)培养12-14d即可观察到明显芽点或瘤状突起,较前人报道的培养时间大大缩短了,4w后芽点进一步发育成丛生芽,芽诱导率达90.2%,每个外植体平均产9个丛尘芽,然后转至培养基MS+BA3 mg/L+AgNO_32 mg/L上诱导芽的伸长,3-4w后可长至3-4cm,切下带有2-3片叶片的幼芽移至生根培养基(MS+NAA0.8mg/L+AgNO_32mg/L),1w后切口处可见白色不定根形成。

It was established that during the period studied choroidal epithelium of NGF and TrKA positive reactivity were present.

研究表明,在胚胎发育期间,脉络丛上皮细胞的分化能诱导脉络丛基质及血管的分化[1]。

The cotyledon explants excised from 5-day-old germfree seedlings were cultured on the MS medium containing 1.0 mg/L 6-BA in darkness for 3 days, and then further cultured under light condition for induction of adventitious buds.

结果显示:将从5 d龄甜瓜无菌苗上切取的子叶外植体接种在MS+6-BA1 mg/L的不定芽诱导培养基上,黑暗条件培养3 d,再转至光下继续培养,诱导出高效不定芽丛。

Hemadynamics changing during the induction and maintenance phase, doses of propofol and atracurium, spontaneous breath recovery time, awake time, time of endotracheal tube extubation and side-effect were investigated and compared. Results There were no significant differences in doses of propofol and atracurium between two groups.

选择ASA 分级Ⅰ-Ⅱ级择期在颈丛复合全身麻醉下行甲状腺次全切除术病人40例,随机分为瑞芬太尼组及芬太尼组,R组采用瑞芬太尼和丙泊酚诱导和维持麻醉,F组采用芬太尼和丙泊酚诱导和维持麻醉,观察并比较两组病人麻醉诱导及维持期血流动力学的变化、术中丙泊酚和阿曲库铵的用量、术毕停药后病人自主呼吸恢复时间、清醒时间、拔管时间以及苏醒期不良反应等。

Methods Forty ASAⅠ-Ⅱpatients scheduled for elective thyroidectomy under cervical plexus block combined with general anesthesia were enrolled and randomly divided into two groups: group remifentanil and group fentanyl. Group R were induced and maintained with remifentanil and propofol, while group F with fentanyl and propofol. Hemadynamics changing during the induction and maintenance phase, doses of propofol and atracurium, spontaneous breath recovery time, awake time, time of endotracheal tube extubation and side-effect were investigated and compared. Results There were no significant differences in doses of propofol and atracurium between two groups.

选择ASA 分级Ⅰ-Ⅱ级择期在颈丛复合全身麻醉下行甲状腺次全切除术病人40例,随机分为瑞芬太尼组及芬太尼组,R组采用瑞芬太尼和丙泊酚诱导和维持麻醉,F组采用芬太尼和丙泊酚诱导和维持麻醉,观察并比较两组病人麻醉诱导及维持期血流动力学的变化、术中丙泊酚和阿曲库铵的用量、术毕停药后病人自主呼吸恢复时间、清醒时间、拔管时间以及苏醒期不良反应等。

Group R were induced and maintained with remifentanil and propofol, while group F with fentanyl and propofol. Hemadynamics changing during the induction and maintenance phase, doses of propofol and atracurium, spontaneous breath recovery time, awake time, time of endotracheal tube extubation and side-effect were investigated and compared. Results There were no significant differences in doses of propofol and atracurium between two groups.

选择ASA 分级Ⅰ-Ⅱ级择期在颈丛复合全身麻醉下行甲状腺次全切除术病人40例,随机分为瑞芬太尼组及芬太尼组,R组采用瑞芬太尼和丙泊酚诱导和维持麻醉,F组采用芬太尼和丙泊酚诱导和维持麻醉,观察并比较两组病人麻醉诱导及维持期血流动力学的变化、术中丙泊酚和阿曲库铵的用量、术毕停药后病人自主呼吸恢复时间、清醒时间、拔管时间以及苏醒期不良反应等。

Methods Forty ASAⅠ-Ⅱpatients scheduled for elective thyroidectomy under cervical plexus block combined with general anesthesia were enrolled and randomly divided into two groups: group remifentanil and group fentanyl. Group R were induced and maintained with remifentanil and propofol, while group F with fentanyl and propofol. Hemadynamics changing during the induction and maintenance phase, doses of propofol and atracurium, spontaneous breath recovery time, awake time, time of endotracheal tube extubation and side-effect were investigated and compared. Results The re were no significant differences in doses of propofol and atracurium between two groups.

选择ASA 分级Ⅰ-Ⅱ级择期在颈丛复合全身麻醉下行甲状腺次全切除术病人40例,随机分为瑞芬太尼组及芬太尼组,R组采用瑞芬太尼和丙泊酚诱导和维持麻醉,F组采用芬太尼和丙泊酚诱导和维持麻醉,观察并比较两组病人麻醉诱导及维持期血流动力学的变化、术中丙泊酚和阿曲库铵的用量、术毕停药后病人自主呼吸恢复时间、清醒时间、拔管时间以及苏醒期不良反应等。

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The split between the two groups can hardly be papered over.

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