诱导

Finally, we demonstrate that human BAMBI can attenuate TGF-β-induced cell growth and cell migration.

最后，我们证实，过量表达BAMBI能够减弱TGF-β诱导的细胞生长阻滞以及促进细胞迁移的作用。

Objective: To clone human augmenter of liver regeneration cDNA, construct the recombinant expression vector, express and purify its product.

目的 ：克隆人肝脏再生增强因子 c DNA，构建重组表达载体并对其诱导表达、纯化。

Objective: To express rALR in GS115 with the constructed pichia pastoris expression plasmid of rat augmenter of liver regeneration and purify it with ultrafiltration and identify its bioactivity in vitro, for studying its biological functions.

目的：利用已构建的大鼠肝再生增强因子毕赤酵母重组表达质粒pPIC9K-rALR，在酵母菌GS115中诱导表达rALR，并进行纯化和体外生物学活性鉴定，为进一步研究rALR的生物学功能提供基础。

To study the expression, purification and bioactivity of human augmenter of liver regeneration in Pichia Pastoris, the expression plasmid pPICZαA- ALR was constructed and transformed into P. Pastoris by the method of electroporation transformation. Induced with 0.5% methanol, the 30 kD protein in the culture supernatant of recombinant P. Pastoris was confirmed to be rhALR by SDS-PAGE and Western blot. Quantitative analysis showed that the target protein was in a level of 66% of the total protein of the culture supernatant, with a yield of 40mg/L.we had performed DEAE anion exchange chromatography two times with excessive and regular adsorption quantity consecutively, and then the rhALR above 95% purity and 52% protein recovery could be obtained by G75 molecular sieve chromatography at last step. The bioactivity assay of the purified product showed that rhALR could stimulate the proliferation of HepG2, SMMC-7721 and NIH-3T3 in vitro.

为在毕赤酵母中分泌表达人肝再生增强因子，以色谱法分离纯化后进行体外活性研究，构建表达载体pPICZαA- ALR，经电穿孔转入毕赤酵母中，用0.5％甲醇诱导表达；重组酵母培养上清经SDS-PAGE电泳和western blot鉴定后表明， rhALR以分子量为30kD的二聚体为主；定量分析结果表明，重组酵母培养上清中rhALR约占总蛋白的66％，表达量约为40mg/L；经DEAE柱和G75柱纯化后，获得的rhALR纯度大于95％，得率为52％；体外生物学活性实验表明，rhALR能明显促进HepG2、SMMC-7721和NIH-3T3细胞的增殖。

Compared with aurum, palladium and platinum as seeds, Silver is much cheaper and lower pollutional and can also activate electroless plating, although it is still rarely investigated.

我们采用激光诱导化学镀技术在聚酰亚(来源：ABC论文1313网www.abclunwen.com)胺薄膜表面上用四种方法选择性沉积金属银作为活化种，然后进行化学镀从而在聚酰亚胺表面上沉积上图形化的铜镀层。

Proteins were prepared by the combination of various extraction methods based on the method of inducer plus autolysis from waste brewer yeast .

本研究在采用诱导剂+自溶法的基础上，结合多种提取方法，通过对比和正交试验确定啤酒废酵母中蛋白质的最佳提取工艺及参数，并用胃蛋白酶-胰蛋白酶联合消化法测定提取蛋白质的消化率。

Background and objective It has been proven that many kinds of drug could induce autophagy in tumor cells.

背景与目的已有的研究表明，很多药物可以诱导肿瘤细胞发生自噬。

Objective:To study the effect of the compound membrane containing bFGF on the autoplastic nerve graft.

在修复长段神经缺损的研究中，多种桥接物(神经桥、肌桥、静脉、基膜、硅胶管等)对再生神经的定向诱导作用已无异议。

The results showed that the best effect of autotetraploid was obtained with 2.0 gL-1 colchicine solution for 6 times, and the frequency was 4.5%.

结果表明：经2.0 gL^(-1)秋水仙素处理6次获得同源四倍体的效果最佳，四倍体诱导率达到4.5%。

The results showed the autotetraploid and triploid cultivars had lower cold resistance than that of diploid under the condition of 12℃ temperature and 1000Lx. With the increase of stress time, the SOD and POD activities of three ploidy cultivars were gradually increased.

低温胁迫对不同倍性西瓜叶片膜脂过氧化及保护酶的影响：通过对蜜枚西瓜幼苗进行低温弱光处理（12℃，光强为10001x，光/暗处理时间为14h/10h）的试验结果表明，在没有冷诱导时M4x、M3x的耐冷性不如M2x。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。