诱导

METHODS: The rhBMP-2,expressed in Escherichia coli, was washed by Triton X- 100 and further purified by DEAE chromatography.The inclusion bodies were resolved in 8 mol/L urea, and were refolded and dimerized in the redox systems (reduced and oxidized glutathione). Finally, a one - step purification procedure based on the heparin affinity chromatography was implemented. The biological activity of purified rhBMP - 2 were tested by induction of the alkaline phosphatase activity in C2C12 cells.

在大肠杆菌中通过温度诱导表达重组人骨形成蛋白-2经过Triton X-100清洗之后，又通过DEAE离子交换层析纯化包涵体，包涵体在8 mol/L尿素变性溶解，在氧化-还原(还原型和氧化型谷胱甘肽)复性系统中，通过简单的稀释复性，通过肝素亲和层析一步纯化法纯化重组人骨形成蛋白-2，最后通过诱导C2C12细胞产生碱性磷酸酶检测重组人骨形成蛋白-2活性。

In our study, we first designed the high and low-degree lens according to the real diopters of guinea pigs, and explored the sensitivity of guinea pigs to lens-induction.

本研究中，我们首次根据豚鼠眼的实际屈光度数设计不同高低度数的诱导透镜，观察豚鼠眼对透镜诱导的敏感性。

The results suggest that the EGFR clustering induced by 50 Hz MF depends on intact lipid rafts on cellular membrane, and the ASM might participate in the process of EGER clustering.

工频磁场诱导FL细胞膜表面EGFR发生聚簇与脂筏结构密切相关，ASM有可能参与了工频磁场诱导的受体聚簇及/或信号转导过程。

By the study of elicitors' action mode on Taxol biosynthesis, their action point in Taxol biosynthesis pathway an...

本文通过对诱导子促进紫杉醇合成的作用模式研究，推导诱导子在紫杉醇合成途径上的作用部位，确定其配伍特性。

The effects of 6 elicitors on taxol biosynthesis and their mechanism were investigated in this paper. The optimum conditions for the elicitors' working were studied, under which, all the 6 elicitors could accelerate taxol accumulation in the cell cultures of T.

研究了6种诱导子在红豆杉培养细胞合成紫杉醇中的调控作用，确定了6种诱导子最佳活性表达条件，并对其作用的生理生化基础进行了初步研究探讨。

The IWFs were purified by (NH4)2SO4 precipitation fractionation, and then applied to Sephadex G-75 column. The column eluates were collected separately according to A280 nm and injected into the health wheat leaves to examine the eliciting activities by PAL, PO assays.

IWF经硫酸铵粗沉淀后，将蛋白沉淀用重蒸水重新溶解后进行凝胶过滤柱层析，以280nm紫外吸收检测、收集不同的蛋白峰，分别注射到健康的Thatcher叶片中，以苯丙氨酸解氨酶(phenylalanine ammonialyase，PAL)、过氧化物酶(peroxidase，PO)防卫反应作为评价诱导活性的指标，检测活性成分在各部分中的分布和诱导活性的强弱。

Epicotyls were cutted longitudinally and cultured in the dark first,on the MS basal medium with 3 mg/L 6-BA and 30 g/L sugar,100% adventitious shoot induction and an average of 33.8 shoots per explant were obtained.

上胚轴纵切结合前期暗培养，在MS附加6-BA 3mg/L、蔗糖30g/L 培养基中，不定芽诱导率为100%，每个外植体平均诱导的不定芽数达33.8

Epicotyls were cutted longitudinally and cultured in the dark first,on the MS basal medium with 3 mg/L 6-BA and 30 g/L sugar,100% adventitious shoot induction and an average of 33.8 shoots per explant were obtained.The base of the adventitious shoots was treated with 1000 mg/ L IBA for 10 min,then placed onto the filter papers which ends dipped into liquid medium with...

上胚轴纵切结合前期暗培养，在MS附加6-BA 3mg/L、蔗糖30g/L培养基中，不定芽诱导率为100%，每个外植体平均诱导的不定芽数达33.8；不定芽在1 000mg/L IBA溶液中浸泡10min后，移入1/4MT+1/2MT+10g/L蔗糖的液体培养基中，采用滤纸桥法进行培养，生根率达100%，平均根数2.5条；移苗成活率达100%。

The effect of AA on BEL-7402 was not significant; However, AA could effectively enhance AT-induced hepatocarcinoma cell apoptosis and lesion severity through activation of caspase-3 but not ERKs-Conclusion; Caspase-3 and ERKs proteins could involve in arsenic-induced hepatocarcinoma cell apoptosis and differentiation respectively as intracellular signaling molecules; The effect between AT and AA on hepatocarcinoma is synergistic, which further inhibits cell growth and induces apoptosis in human hepatocarcinoma cells through activation of caspase-3 but not ERRs.

AA对BEL-7402无明显的作用，但能有效的通过caspase-3而不是ERKs的激活促进AT对肝癌细胞的凋亡效应。结论：ERKs和caspse-3信号蛋白可能分别参与了砷剂诱导的人肝癌细胞促分化和凋亡效应，AT和AA对肝癌细胞的作用有协同性，他们通过caaspase-3而不是ERKs的激活进一步抑制了肝癌的生长，诱导其凋亡。

Methods Basolateral amygdala slices of rats were prepared. Field excitatory post-synaptic potentials of BLA were recorded by stimulating the external capsule. Two TBS's were applied to induce LTP in BLA. Each TBS included a brief, high-frequency pulse train (5 pulses at 100 Hz) given at the theta-rhythm (5Hz) for 4 seconds. Experiments compare the effects of different intervals of two TBS's on the expression of LTP in BLA.

制备杏仁核脑片，刺激外囊记录BLA场电位，应用两串θ频率波刺激诱导LTP，每串θ频率波刺激为20个(频率5Hz)短时间高频串脉冲(5个脉冲，频率为100Hz)，通过改变两串θ频率波的刺激间隔，分析不同参数诱导的LTP是否存在差异，并在灌流的人工脑脊液中加入TPK抑制剂genistein，观察其对杏仁核LTP的影响。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。