诱导

Part Ⅳ Further Studies on Effects of Anti-S. japonicum Egg Granuloma and Hepatic Fibrosis Induced by SCP Combining Reagent Further to probe the effects of anti-S. japonica and antiS. japonicum hepatic fibrosis induced by shark cartilage powder cooperating"425"preparation.

第四部分 SCP合剂诱导的抗血吸虫卵肉芽肿及抗肝纤维化效应的进一步研究为进一步探讨鲨鱼软骨粉联用&425&制剂诱导的抗血吸虫卵肉芽肿及抗血吸虫性纤维化效应。

Allogeneic and syngeneic fresh bone, autolyzed antigen-extracted bone, bone matrix gelatin, demineralized bone matrix were implanted into the muscle pouch of mice, at different times after implantation, the immunological, histological and alkaline phosphatase assay were conducted. The results revealed that all four kinds of allogeneic implants activated specific cellular and humoral immune responses, most notably in fresh bone group, the AAA, BMG and DBM inhibited the proliferation of the lymphocytes in vitro and BMG had the most powerful inhibiting action, allogeneic AAA, BMG and DBM might induce heterotopic osteogenesis in vivo, however, there were obvious difference in ALP and histomorphometry between allografts and isografts.

在小鼠肌肉内植入同种异体或同系新鲜骨、自消化抗原去除骨、骨基质明胶、脱钙骨基质，术后免疫学、碱性磷酸酶、组织学检查发现：4种同种异体植入物均可引发特异性细胞免疫反应和体液免疫反应，其中以新鲜骨移植引发的免疫反应最为显著；自消化抗原去除骨、骨基质明胶、脱钙骨基质在体外具有抑制淋巴细胞增殖的作用，骨基质明胶对淋巴细胞增殖的抑制作用最显著；同种异体与同系4种植入物在体内成骨活性有显著差异；自消化抗原去除骨、骨基质明胶、脱钙骨基质均有异位诱导成骨活性，其中骨基质明胶骨诱导活性最佳。

Inducing earlier is a consummate condition and method of culturing and inducing in vitro.

早期诱导是较为完善的体外诱导培养条件和方法。

The thesis mentioned that the conformation of lipase molecular can be induced by substrate to be an activated one in organic phase through constructing a water-in-oil micro-emulsion system. The activated conformation was recorded using high cross linking degree polymer. The effect of inducement on the activity of recorded lipase polymer was studied, the catalyzed property of recorded lipase polymer in water phase was investigated, and the fluorescence spectrum of lipase solution and recorded lipase polymer under different kinds of denaturalized circumstance were analyzed.

本文提出通过构建油包水型微乳体系，使酶分子能够在有机相中被反应底物诱导出活性构象，利用高交联度聚合物对该构象进行刻录，比较了底物诱导对刻录酶活性的影响，考察了刻录酶在水相中催化特性，并对脂肪酶溶液和刻录酶聚合物在各种变性条件下的荧光光谱进行分析。

It is concluded that the Ag granules deoxidized in solution infiltrated into substrate while depositing on surface of substrate.

目前，国内外多数使用短波长的激光(如Ar+、氦氖及Nd：YAG等)作为诱导光源，从各种介质中诱导金属沉积[4]。

Here we present evidence that NMDA receptors not only contribute to normal synaptic responses induced by stimulation of local layer V or white matters, but also contribute to generation of action potentials induced by a depolarizing step applied to the soma.

我们的研究表明，NMDA受体不仅参与刺激前扣带皮层的第五层细胞或刺激白质诱导的突触反应，而且参与在胞体施加去极化跃阶电流诱导的动作电位的发放。

Using whole-cell patch clamp recording, we observed multiple distinct ATPinduced currents in isolated DRG neurons of adult rats, each with unique desensitizing kinetics.β,γ-meATP, agonist of P2X1 receptor also induced fast inactivating and slowly desensitization current in small DRG neurons. PDBu, a selective agonist for PKC, decreased the P2X receptor-mediated inward currents in a dose-dependent manner, while SNP, nitric oxide donor, potentiated the ATP-evoked current through NO-cGMP pathway.

全细胞膜片钳记录观察到，在成年鼠离体DRG伤害性感受相关的小直径神经元上，ATP可诱导出多种具不同反应型式及脱敏动力学的由P2X受体介导的内向流；P2X1受体激动剂β，γ-meATP亦可诱导出快脱敏及慢脱敏两种型式的内向流；PKC激动剂可剂量依赖地削弱P2X受体介导的内向流，而NO供体硝普钠可通过NO-cGMP通途显著易化ATP介导的内向流。

In Part 1 of our research, BGSCs were sorted through immunomagnetic beads marking by CD133 and cultured in vitro, and character as a stem cell was identified by stem cell markers (CD133 and Nestin) and differentiated cell markers [ microtubule-associated protein 2(MAP2), glial acidic fibrillary protein and myelin basic protein] , ultrastructure observing with electron microscopeand engrafting to severe combined immunodeficiency mice for tumorigenesis test. The results were as following: Only a small subset of CD133+ glioma cells in glioma cell lines and fresh specimens from various pathologic grade could express stem cell markers CD133 and Nestin, view ultrastructure of a stem cell and be capacity of serial passage in culture. These CD133+ cells possese a marked capacity for multipotent differentiation and could differentiate into tumor cells expressing MAP2,β-TubulinⅢ, GFAP and MBP; When engrafted into SCID mice, they can generate and form tumors that phenotypically resembl the tumor from the patient.

在本课题中，在第一部分实验中采用以CD133为标志的免疫磁珠法从人脑胶质瘤组织和细胞株中分离脑胶质瘤干细胞并进行体外培养，通过免疫荧光技术检测干细胞标志物CD133、Nestin，诱导分化后检查分化细胞标志物MAP2、GFAP、MBP以及电镜超微结构观察和移植SCID鼠致瘤实验，对其干细胞特性加以鉴定，得到如下结果：不同病理分级的新鲜胶质瘤标本和胶质瘤细胞株中存在一小部分CD133+的胶质瘤细胞，能表达干细胞的标志物CD133和Nestin，符合干细胞的超微结构特点，体外培养能连续传代；具有多向分化潜能：诱导分化后能产生MAP2、β-TubulinⅢ、GFAP、MBP染色阳性的细胞；移植SCID鼠后能形成与亲本肿瘤表型一致的移植瘤。

AIM: To investigate the expression of human β-defensin-2 (hBD-2) mRNA and the changes of NF-κB activity induced by lipopolysaccharide in human primary epitheliums. METHODS: The pulmonary primary epitheliums from human were stimulated with LPS and the expression of hBD-2 mRNA was detected by reverse transcription polymerase chain reaction.

目的：观察脂多糖诱导人气道上皮细胞人β防御素－2(hBD-2)的表达及核转录因子κB活性的变化，探讨NF-κB在LPS诱导人气道上皮细胞hBD-2表达中的作用。

Flow cytometry analysis showed that the 1% and 2% of lactic acid bacteria fermentation filtrates induced the apoptosis of Hela cells at 48 h and 72 h, while the 5% of the filtrate induced the apoptosis at 24 h, 48 h and 72 h, respectively.

流式细胞仪分析，1%、2%的乳酸杆菌DM9811发酵滤液在48、72h可诱导Hela细胞凋亡；5%的乳酸杆菌发酵滤液在24、48和72h均可诱导Hela细胞凋亡。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。