诱导

ERAF17 gene expresses differently between male and female reproductive organs. Its expression is induced by ethylene or ethylene released compounds that can promote femaleness. No evidence directly shows the relationship between ERAF17 gene and femaleness, however. So this work focus on proving the positive correlation between the expression of ERAF17 gene induced by ethylene and development of female flowers,then search for the signal pathway of cucumber female reproductive organ development.

ERAF17基因在黄瓜的雌雄生殖器官的表达不同，可以由乙烯诱导表达，从而推动雌性化，但是它的作用机制却不清楚，并没有明确的证据表明ERAF17基因与雌花的发育有直接关系，所以本论文研究重点在于证明乙烯诱导ERAF17基因的表达对黄瓜雌花的分化有确定的正相关，从而可以探索黄瓜雌性器官发育存在的可能的信号通路，并对应用前景进行评估。

The adhesion test showed that small intestinal epithelium cells of 30-35-day-old postweaning piglets with both genotype M307~ and M307~ could adhere to the standard E.coli strain express F18ab fimbriae, the recombinant E.coli express F18ac rE.coli1534 and the recombinant pnirBMisL-fedF E.coli displaying FedF subunit on the surface of E.coli, but small intestinal epithelium cells with genotype M307~ and 3-day-old piglet could not adhere to the three kinds of bacteria descried above, the latter with good adherence capability of 987P fimbrial E.coli.

9仔猪小肠上皮细胞的黏附试验结果显示，30～35日龄M307~基因型和M307~基因型断奶仔猪的小肠上皮细胞均能与表达F18ab菌毛标准菌株107／86、诱导表达F18ac菌毛的重组大肠杆菌rE.coli1534及诱导菌体表面展示表达F18黏附亚单位FedF的重组大肠杆菌pnirBMisL-fedF发生黏附作用，而同日龄M307~基因型断奶仔猪的小肠上皮细胞均不能与上述三株大肠杆菌发生黏附作用。3日龄易感仔猪小肠上皮细胞也不能黏附上述三株大肠杆菌，但可以很好地黏附表达于987P菌毛的大肠杆菌。

The expression of BjCHI1 was highly inducible by methyl jasmonate treatment, wounding, caterpillar feeding, and pathogenic fugal infection. These observations suggest that the promoter of BjCHI1 gene might contain specific cis-acting elements for stress responses.

BjCHI1基因受伤害、茉莉酸甲酯、虫食、真菌侵染等的强烈诱导表达，这暗示BjCHI1启动子可能含有相应的诱导元件。

In fundic strips, ghrelin and GHRP-6 could decrease the on-response induced relaxation and increase off-response induced contraction of the muscle, with the effect of Ghrelin obviously stronger than that of GHRP-6.L-NNA could increase the effects of Ghrelin and GHRP-6-induced muscle contraction, and L-AA could decrease their effects.

在胃底部，Ghrelin和GHRP-6可使开电刺激诱导的平滑肌舒张活动减弱，断电刺激诱导的肌条收缩活动增强，且Ghrelin作用明显强于GHRP-6.L-NNA可显著增强Ghrelin和GHRP-6的促平滑肌收缩效应，但L-AA可显著减弱该作用。

The off-contractions were also cholinergically mediated as they disappeared under non-adrenergic,non-cholinergic conditions using atropine and guanethidine.In fundic strips,ghrelin and GHRP-6 induced on-relaxation and off-contraction and both ghrelin and GHRP-6 may significantly reduced on-response and increased off-response.

在胃底部，Ghrelin和GHRP-6可使on-response诱导的平滑肌舒张活动减弱，off-response诱导的肌条收缩活动增强，且Ghrelin作用明显强于GHRP-6.L-NNA可显著增强Ghrelin和GHRP-6的促平滑肌收缩效应，但L-AA可显著减弱该作用。

However, the binding patterns between the HU-induced and uninduced HEL cells were different. The data in Southwestern-blot analysis showed that the molecular weights of the GATA factors binding to the HS2core DNA sequence were 43kd and 47kd respectively.

反义寡核苷酸技术及RT-PCR的结果表明，GATA-1的反义核苷酸抑制了羟基脲诱导的β-珠蛋白基因的表达，进一步证明了GATA-1因子在羟基脲诱导的HEL细胞分化过程中是不可缺少的促进因素。

Gentiana total glycosides can induce apoptosis of SMMC-7721 cells and U937, whereas there was no A2780 cells apoptosis induction.

一定浓度的秦艽总苷可诱导SMMC-7721细胞和U937细胞凋亡；对A2780无诱导凋亡作用。

The cotyledon explants excised from 5-day-old germfree seedlings were cultured on the MS medium containing 1.0 mg/L 6-BA in darkness for 3 days, and then further cultured under light condition for induction of adventitious buds.

结果显示：将从5 d龄甜瓜无菌苗上切取的子叶外植体接种在MS+6-BA1 mg/L的不定芽诱导培养基上，黑暗条件培养3 d，再转至光下继续培养，诱导出高效不定芽丛。

The best explant in the culture was brown leaf,and the explant sterilized in 0.1% aqueous mercuric chloride for 5min has the best effect;At the initial stage in induction,3% glucosan have the best effect following with 1% glucosan+2%sucrose are optimum in the process of culturing;the optimum culture medium is 1/2MS; the preferable medium of callus is 1/2MS+ KT(0.1mg/L)+6-BA(0.05mg/L)+2.4-D(0.4mg/l);differentiation1/2MS+6-BA(0.3mg/l)+NAA0.2+KT(0.05mg/l);and the preferable medium of rooting is :1/2MS+NAA(0.05mg/L).

安祖花组织培养的最佳外植体为棕色叶片；最佳消毒时间为0.1%的升汞消毒5min；最佳碳源：诱导初期3%的葡萄糖的诱导效果最佳，后期培养以2%蔗糖+1%葡萄糖的培养效果较好；最佳基本培养基为1/2MS。

First passage of human amniotic epithelial cells were inoculated at a density of 1×108/L and cultured in medium containing fetal bovine serum of 0.1 volume fraction, 100 nmol/L dexamethasone, 50 mg/L ascorbic acid and 5 mmol/L β-glycerophosphate.

取第1代人羊膜上皮细胞，设立两组：诱导组以1×108 L-1密度接种于培养皿内，24 h后更换为含体积分数为0.1的胎牛血清、 100 nmol/L地塞米松、50 mg/L抗坏血酸、5 mmol/Lβ-甘油磷酸的诱导培养液。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。