诱导

The first passage of YS-MSCs which grew in good condition was obtained, and adipogenic differentiation of YS-MSCs was induced by dexamethasone and insulin, and angioblast was induced by vascular endothelial growth factor and basic fibroblast growth factor.

取生长状态良好的第1代细胞，以地塞米松、胰岛素定向诱导卵黄囊间充质干细胞向脂肪细胞方向分化；以血管内皮生长因子和碱性成纤维细胞生长因子体外诱导其向血管内皮细胞方向分化。

Nerve stem cell can be differentiation into astroblast, oligodendrocyte and nerve cell induced by factors.

神经干细胞的增殖分化可受各种因素的影响，目前已知，许多细胞因子，生长因子，营养因子以及细胞外环境等因素的作用，可诱导神经干细胞向不同的终末细胞分化，若能在体外分离培养神经干细胞并在促分化因子的作用下，诱导神经干细胞向某一特定方向分化，将为干细胞的分化机制提供有力的实验依据。

Key words:Dendrobium candidum; Axenic nodal segments; In vitro propagation; Concentration of hormone; Organic susbstance

方法将铁皮石斛试管苗茎节接种于不同NAA浓度的MS培养基上诱导腋芽萌发，并在含不同激素配比和有机添加物的培养基上继代和诱导生根。

In the study, embryo bud of Impatiens balsamina L was cultured on hormone-free MS medium for promoting, MS medium with 6-BA 3 mg/L+NAA 0.5 mg/L for shoot and root formation, and MS medium containing 6-BA 3 mg/L+NAA 0.5 mg/L for flowering respectively. During about 60 days, plant regeneration and flowering of Impatiens baisamina L was finished in vitro.

研究以凤仙花无菌苗胚芽段作为外植体，经过不含任何激素的MS(0)培养基预培养、MS+6-BA3mg/L+NAA0.5mg/L培养基的诱导芽和根分化、MS+6-BA1mg/L+NAA0.1mg/L培养基的诱导开花，在60d左右实现了凤仙花离体培养再生植株并在试管内开花。

After treatment of benzamide which is an inhibitor of poly polymerase,β-galactosidase activity assays and PCR analysis were performed to test the deletion of LacZ gene and c-Ha-T24ras gene. The results showed that:(1)LacZ gene was deleted from both A13.4-NIH3T3 cells and B12.7-NIH3T3 cells, but neigher from A13.4-HeLa cells nor B12.7-HeLa cells. So it is possible that there is cell line specificity in gene deletion induced by BA.(2) In A13.4-NIH3T3 cells, LacZ gene and c-Ha-T24ras gene were completely deleted at the same time.(3) The transcription activity of exgenous DNA fragment may have same effect on its sensitivity to the deletion induced by BA.

用聚ADP核糖聚合酶的NAD位点抑制剂苯甲酰胺分别处理四种转化细胞后，检测细胞中整合的外源LacZ基因与c-Ha-T24ras基因的删除情况，结果如下：BA诱导的基因删除可发生于NIH3T3转化细胞系，但不能发生于HeLa转化细胞系；位于同一外源表达载体上的LacZ基因与c-Ha-T24ras基因的删除过程是同步的；外源整合DNA片段的转录强度可能直接影响其被BA诱导删除的敏感性。

The soluble peptidoglycan is gained by short dose benzylpenicillin sodium and sephadex G-100; the SLP reagent kit revealed the prepared soluble peptidoglycan is peptidoglycan; the limulus test showed that the content of LPS in the prepared soluble peptidoglycan(10ng/ml) is lower (0.01ng/ml); the improved LOWRY reagent kit showed the content of protein in the prepared soluble peptidoglycan(1.0mg/ml) was 0.06mg/ml; the optimization condition is 0.75mg/ml of the dosage of benzylpenicillin sodium,1h of the incubation time of benzylpenicillin sodium addition and 5×108CFU/ml of the bacterial concentration.

应用青霉素诱导和Sephadex G-100凝胶柱成功分离得到金葡菌可溶性PGN；所制备的可溶性PGN经SLP试剂检测结果为阳性；动态浊度鲎实验检测制备的可溶性PGN(10ng/ml)中LPS含量小于0.01ng/ml；改良LOWRY试剂检测可溶性PGN(1.0mg/ml)中蛋白含量为0.06mg/ml；青霉素诱导和Sephadex G-100凝胶柱法提取金葡菌可溶性PGN的最佳条件是：青霉素的使用浓度为0.75mg/ml，菌液浓度为5×108CFU/ml，加入青霉素后孵育时间为1h。

The results show that the porosity of the membrane prepared by ScCO_2 induced phase separation is higher than that prepared by conventional method;the membrane structures are significantly influenced by the process parameters;compared with the conventional immersion precipitation phase inversion,ScCO_2 induced phase separation process can effectively avoid the formation of macrovoids at suitable operating conditions;during depressurization process,the great differences in the effect of depressurization time on membranes structure for various polymer materials result from that the speed of CO_2 gaseous nuclei escaping from membrane substrate can not keep the pace with the outer depressurization rate.(2) The tendency that the interaction parameters affect the binodal curve position is illustrated by changing the interaction parameters in reasonable ranges.

研究结果表明：与传统方法相比，采用ScCO_2诱导相转化法可获得更高的膜孔隙率；通过改变操作参数可对膜的结构进行调控，且操作参数对膜结构的影响与铸膜体系的自身性质也紧密相关；在适宜的操作条件下，ScCO_2诱导相转化法能有效避免采用传统的浸入沉淀相转化法制膜时易出现的大空腔结构；泄压过程中CO_2气核从聚合物膜基体中逃逸的速度能否与膜基体外部的泄压速度保持同步，是泄压时间对不同材质膜结构影响截然不同的根源所在。

To study the similarities and differences between male sterility induced by physiology and heredity, different male-sterile materials induced by heredity [S-type male-sterile line ms2611, ms1376] and physiology (induced by high temperature and chemical hybridizing agents SQ-1), Xinong 1376 and Xinong 2611 were used as materials, and their spikes at mononucleated prophase stage, anther and flag leaf at mononucleated anaphase stage, binucleated stage and trinucleated stage were used to conduct protein analysis.

为了研究小麦遗传型和生理型雄性不育在生化机制上的异同，以遗传型［S型不育系ms2611和ms1376］和生理型雄性不育小麦西农2611和西农1376（高温诱导和化学杀雄剂SQ-1诱导的雄性不育）为材料，分别取其小孢子处于单核早期的幼穗，单核后期、二核期、三核期的花药及旗叶进行了可溶性蛋白质的比较研究。

Results: BIO-1211 aerosol at 1, 3 and 10 mg/ml significantly inhibited the changes in lung resistance and lung dynamic compliance after antigen challenge in the sensitized rats in a dose-dependent manner, BIO-1211 at 25, 50, 100 and 200μg/ml inhibited the fibronectin-induced neutrophil adhesion by 23.5%, 24.6%, 61.4% and 58.1%, respectively, and serum-induced adhesion by 29.9%, 35.9%, 35.3% and 15.4%, respectively. Inhalation of 10mg/ml BIO-1211 did not show any protection against histamine and actetylcholin-induced bronchoconstriction.

结果：给大鼠气雾吸入BIO-1211 (1、3、10mg/ml)呈剂量依赖抑制致敏大鼠抗原攻击引起RL的升高和Cdyn降低；BIO-1211(25、50、100、200μg/ml)明显抑制纤维黏连蛋白和血清诱导的中性粒细胞黏附反应，其抑制率分别为23.5%、24.6%、61.4%、58.1%和29.9%、35.9%、35.3%、15.4%；但BIO-1211 (10 mg/ml)给豚鼠气雾吸入不能抑制乙酰胆碱和组胺诱导的药物性哮喘。

In addition, the time of buds induction directly from leaf is shorter than those from calli.

并在时间上，从芽的长势、芽的生根情况进行比较，结果表明：直接诱导的不定芽比诱导愈伤组织分化的不定芽所需的时间短、长势好、生根快。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。