诱导

The profile of liver CYP450 induction by DEX in male rat After 4 daily i.p. administering of 0,25, 50 and lOOmg/kg/d DEX in male Wistar rat, it was determined the total CYP450 content, the mRNA and protein expression levels of CYP3A1, CYP3A2 and CYP2B1/2, and the ERD (CYP3A catalytic activity), PROD (CYP2B catalytic activity) and BROD (total CYP450 catalytic activity) in livers.

DEX对雄性大鼠肝脏CYP450的诱导效应雄性Wistar大鼠腹注0、25、50和100mg DEX/kg/d诱导处理4天后，测定大鼠肝脏总CYP450含量，CYP3A1、CYP3A2和CYP2B1/2的mRNA及蛋白表达水平，肝脏红酶素脱甲基酶(ERD，CYP3A活性)，苄氧基试卤灵脱乙基酶(PROD，CYP2B活性)，苯氧基试卤灵脱乙基酶(BROD，总CYP450活性)，结果表明，CYP450含量、ERD、PROD和BROD在DEX多次诱导后都有升高，CYP3A1 mRNA表达水平、蛋白含量和酶活性有明显的升高，剂量效应关系明显；CYP3A2蛋白也有明显升高，而其mRNA表达水平却没有变化，提示CYP3A1和CYP3A2的诱导机制可能不同。

The profile of liver CYP450 induction by DEX in male ratAfter 4 daily i.p. administering of 0,25, 50 and lOOmg/kg/d DEX in male Wistar rat, it was determined the total CYP450 content, the mRNA and protein expression levels of CYP3A1, CYP3A2 and CYP2B1/2, and the ERD (CYP3A catalytic activity), PROD (CYP2B catalytic activity) and BROD (total CYP450 catalytic activity) in livers.

DEX对雄性大鼠肝脏CYP450的诱导效应雄性Wistar大鼠腹注0、25、50和100mg DEX/kg/d诱导处理4天后，测定大鼠肝脏总CYP450含量，CYP3A1、CYP3A2和CYP2B1/2的mRNA及蛋白表达水平，肝脏红酶素脱甲基酶(ERD，CYP3A活性)，苄氧基试卤灵脱乙基酶(PROD，CYP2B活性)，苯氧基试卤灵脱乙基酶(BROD，总CYP450活性)，结果表明，CYP450含量、ERD、PROD和BROD在DEX多次诱导后都有升高，CYP3A1 mRNA表达水平、蛋白含量和酶活性有明显的升高，剂量效应关系明显；CYP3A2蛋白也有明显升高，而其mRNA表达水平却没有变化，提示CYP3A1和CYP3A2的诱导机制可能不同。

Study on the rapid Propagation Technique of Lycoris. Herb and get follow results: In the period found of axenic clone 0.1%HgCl2 is the best disinfector to deal with the Lycoris" bulb ,as to neat part, such as root, leave, and bulbil, is fit to use 0.1%HgCl2 antisepsis 7min. And found the best effect is the bulb scale with base. Root, leaf and bulb scale without base all were not inducement adventitious buds. Different position of bulb had different culture effect. 3-15 of middle part of bulb can be induced most adventitious buds but inner and outer of least it. Incised the bulb with three types (pieces of eight, pieces of six, piece of four), and found the type of pieces of six is best to Lycoris mass production. L.sprengeri is fit MS+BA5mg/l+NAA0.1mg/l and L.squamigera is fit MS+BA5mg/l+NAA0.5mg/l, but the medium fit to culture L.longituba haven"t be found.In the period of Subculture-Found of mass production, the most multiplication of adventitious buds in MS+BA5mg/l+NAA0.5mg/l during subculture of L.sprengeri, L.squamigera.

石蒜属植物快速繁殖技术研究中，在无菌无性系建立阶段：鳞茎以0.1％HgCl_2消毒10-12分钟效果最好，而叶、根尖、鳞芽等较干净部位选用0.1％HgCl_2消毒7分钟；用三种石蒜属植物的叶片、根尖、鳞芽及带基盘与不带基盘鳞片进行培养，以带基盘鳞片诱导分化效果最理想，鳞芽易培养出芽，但数量有限，而叶片、根尖与不带基盘鳞片均未诱导分化；带基盘鳞片为石蒜属植物快速繁殖最佳外植体，以鳞茎中部3-15层芽诱导率高，较外层稍次之，内部鳞片诱导率最低；选用八等分法、六等分法、四等分法切割鳞茎，六等分法综合效果最好；每外植体带三鳞片培养最为适宜；三种石蒜属植物各自适合的培养基成分不同，换锦花在MS+BA5mg／l+NAA0.1mg／l中培养效果最好，夏水仙为MS+BA5mg／l+NAA0.5mg／l，而白花长筒石蒜在各培养基组合中培养效果均不理想，其适合的培养条件有待于进一步研究。

The effect of bud-inducing and multiplication was better on the medium MS+6-BA2.0mg/L+NAA0.2mg/L,and the inducing rate of asepsis plantlet was 80%, the inducing rate of clumpy buds was 100％.

在以MS+6-BA2.0mg/L+NAA0.2mg/L的培养基中进行的不定芽诱导生长和继代培养，诱导和增殖效果较好，丛生芽的一代诱导率为80%，二代以后丛生芽诱导率为100%。

Recombinant ptotein made up 39.2%(pET-20b-bglA) and 33.5%(pET-28a-bglA) of the total proteins in the intracellular fraction, the solubility proportion of the enzyme up to 32.8%(pET-20b-bglA) and 40.1%(pET-28a-bglA), the activities of the enzyme were 66.8 (pET-20b-bglA) and 71.2 U/mg (pET-28a-bglA). These results showed that E. coli BL21-CodonPlus (DE3)-RIL with argU tRNA, ileY tRNA and leuW tRNA genes helped to improve expression of the enzyme through enhanced identification of the rare codons AGA/AGG, AUA and CUA. Further optimized the conditions for inducing, the solubility proportion of the enzyme was 70.6% at 16 °C and 1.2 times higher than 37 °C. The solubility proportion of the enzyme increased from 12.3% to 32.8% when IPTG concentrations dropped from 1000 μM to 25 μM. The recombinant enzyme was purified by heat treatment, DEAE Sepharose anion-exchange chromatography and TOYOPEARL HW-55F.

maritima MSB8 的-葡萄糖苷酶基因 bglA克隆至表达载体 pET-20b 和 pET-28a，构建重组质粒 pET-20b-bglA 和 pET-28a-bglA，然后转化不同大肠杆菌 E.coli 宿主，Tm-SIGlA 在 E.coli BL21-CodonPlus(DE3)-RIL 中获得高效表达，重组蛋白的表达量为 33.5%(pET-28a-bglA)和 39.2%(pET-20b-bglA)，可溶性比例为 32.8%(pET-20b-bglA)和 40.1%(pET-28a-bglA)，比酶活达 66.8 (pET-20b-bglA)和 71.2 U/mg (pET-28a-bglA)，这些结果表明，E.coli BL21-CodonPlus(DE3)-RIL 宿主带有的 argU tRNA、ileY tRNA 和 leuW tRNA 基因，分别增强对稀有密码子 AGA/AGG、AUA 和 CUA 的识别，有助于提高该酶在 E.coli 中的表达；进一步优化诱导条件，重组 E.coli BL21-CodonPlus(DE3)-RIL/pET-20b-bglA 在 37 ℃下诱导培养，IPTG 浓度由 1000 μM 降至 25 μM，目的蛋白可溶性表达由 12.3%增至 32.8%，提高 1.7 倍，16 ℃下低温诱导实现目的蛋白 70.6%的可溶性表达，较 37 ℃下诱导培养提高 1.2 倍。

OBJECTIVE: To establish an inducing system for differentiation of bone marrow mesenchymal stem cells into chondrecytes in two-dimensional culture in vivo, to analyze the best concentration of transforming growth favor beta-1 (TGF-β1) to induce the differentiation and the correlated influence factors for the directional differentiation, and to observe the changes of cell form and phenotype.

目的：建立二维培养条件下骨髓间充质干细胞分化为软骨细胞的诱导体系，分析转化生长因子β1作为诱导条件的最佳浓度，以及诱导骨髓间充质干细胞定向分化为软骨细胞的相关影响因素，观察细胞诱导后的细胞形态及表型变化。

OBJECTIVE: To establish an inducing system for differentiation of bone marrow mesenchymal stem cells into chondrecytes in two-dimensional culture in vivo, to analyze tbe best concentration of transforming growth favor beta-1 (TGF-β1) to induce tbe differentiation and the correlated influence factors for the directional differentiation, and to observe the changes of cell form and phenotype.

目的：建立二维培养条件下骨髓间充质干细胞分化为软骨细胞的诱导体系，分析转化生长因子β 1作为诱导条件的最佳浓度，以及诱导骨髓间充质干细胞定向分化为软骨细胞的相关影响因素，观察细胞诱导后的细胞形态及表型变化。

RESULTS: Cells were spindle shape before induction, obviously varied following preinduction, and then the body began contracted after being induced in the medium for 1 hour, with the presence of a few small orbicular-ovate or spindle cells. Following 24 hours of induction, about 60% cells became double pole, multipolar or cone shape, showing neuron-like structure.

结果：①诱导分化前呈梭形细胞，在加入预诱导培养基后未出现明显的形态变化，换用诱导培养基1 h，胞体开始收缩，出现少数较小的卵圆形或纺锤形细胞，诱导24 h后，约60%细胞变成双极形、多极形和锥形，出现类似神经元细胞的形态。

Result: The inductivity of leaves was the highest about 87.5%, followed with the stem section and leafstalk; The inductivity of nutrient medium such as MS, B5 callus was higher than the ones like H, SH and the White callus amended one; It was found that low-grade Phvalue benefits the growth of callus. The experiment result showed that different pH showed little difference in quality. The best condition of culture was 25 ℃in temperature. Conclusion: The best culture condition for callus was the leaves as explantation.

结果：叶片的诱导率最高，为87.5%；茎段次之，叶柄较差；培养基MS，B5愈伤组织诱导率较高，达到了80%以上，高于培养基H，SH和改良White的愈伤组织诱导率；偏低的pH对愈伤组织生长有利，出愈率高，但从愈伤组织生长质量方面看，不同pH差异不明显；愈伤组织诱导最适温度为25 ℃。

Then, with some examples, the problems of directional indicator design for underground space are discussed systematically from angles of space inductive design and indicator sign system.

本文从理论上阐明了地下空间方向诱导设计的必要性，介绍了定位定向的相关理论，然后结合实例，从空间诱导设计和标示系统诱导设计两大方面对地下空间方向诱导设计的问题进行了较为系统的探讨。

The absorption and distribution of chromium were studied in ryeusing nutrient culture technique and pot experiment.

采用不同浓度K2CrO4(0，0.4，0.8和1.2 mmol/L)的Hoagland营养液处理黑麦幼苗，测定铬在黑麦体内的亚细胞分布、铬化学形态及不同部位的积累。

By analyzing theory foundation of mathematical morphology in the digital image processing, researching morphology arithmetic of the binary Image, discussing two basic forms for the least structure element: dilation and erosion.

通过分析数学形态学在图像中的理论基础，研究二值图像的形态分析算法，探讨最小结构元素的两种基本形态：膨胀和腐蚀；分析了数学形态学复杂算法的基本原理，把数学形态学的部分并行处理理念引入到家实际应用中。