诱导

Cells in the induction group were treated with L-DMEM consisting of 10% fetal calf serum and 10 μg/L basic fibroblast growth factor for 24 hours, then induced with L-DMEM consisting of 2% dimethyl sulphoxide and 200 μmol/L butyl hydroxy anisol for 1, 3 and 5 hours.

设立2组，诱导组用含10%胎牛血清和10 μg/L成纤维生长因子的L-DMEM培养基预诱导24 h后，再以含2%二甲基亚砜和200 μmol/L丁羟基茴香醚的L-DMEM无血清培养基诱导1，3，5 h。

NodD binds to and bends target promoters through anchoring two tandem and individual specific DNA sites. NodD functions as a tetramer, which has a V-shaped main body. Tetrameric NodD is to change its own conformation rather than its oligomeric forms in response to small signal molecules. The specific interaction between each NodD DNA-binding domain and each specific DNA site does not alter itself in spite of naringenin induction, and the induced conformational change is transferred from protein to DNA. Only the DNA conformation incited by induced NodD is competent for RNA polymerase to form the transcriptional open complex. It cannot be excluded that NodD may have protein-to-protein contacts with RNA polymerase, and that the NodD conformational change may also directly contribute to the transcriptional open complex formation. However, the NodD conformational change itself cannot serve as the determinant of the transcriptional molecular switch.

通过研究，我们提出了初步的NodD操纵子激活模型：第一，四聚体是NodD蛋白的功能单位，它通过铆钉两个串联的相对独立的DNA靶位点结合被诱导的启动子；第二，小分子配基的结合是改变NodD四聚体的构象而不是引发不同形式的寡聚体，在我们的模型中，NodD四聚体缩小其V形主体的弯折角，进而缩短其DNA结合功能域的间距；第三，小分子信号的诱导并没有改变NodD的DNA结合域和其DNA靶位点的相互作用，NodD的构象改变由蛋白质经其双铆钉位点传递给DNA；第四，只有诱导状态的NodD激发的DNA构象才能有效地使RNA聚合酶形成转录开放复合物；第五，不排除NodD与RNA聚合酶可能有直接的相互接触位点，不排除NodD构象的改变可能直接有利于RNA聚合酶形成转录开放复合物，但是我们认为NodD构象改变本身不是充当转录激活开关的决定因素。

These results indicated that the efficient tuberization of potato could be obtained by using a half-solid medium.The half-solid medium took shorter period to tuberize,produced higher tuberizing rate,costed less and was more suitable for mass production of potato microtubers in large containers.And 0.1mg/Lpaclobutrazol could effectively promote the yield and quality of potato microtubers.But different cultivar for paclobutrazol treatment had genetic differences in microtuber inducement.

试验结果表明，两种培养基都以半固体方式培养诱导试管微型薯效果最好，结薯率高，成本低，适用于在大容器内规模化培养诱导生产微型薯；在马铃薯试管微型薯生产中，诱导条件下添加0.1mg/L多效唑，可以提高试管薯的产量和质量，但存在基因型差异。

However, higher concentration of CRP may increase the expression of protein and mRNA of inflammatory factor in endothelial cells; 2 Higher concentration of CRP may participate in the development of inflammatory process in endothelium by the mechanism that CRP induce cells apoptosis and activate signal pathway involved in cell proliferation. 3 CRP is the member of pentraxins family, in which two faces of pentamer form a special bi-phase structure: one face is ligand recognition phase; the other face is effector phase.

总结： 1CRP是一种保守蛋白，在正常生理水平下对内皮细胞没有明显的诱导炎症发生的作用，仅在高浓度条件下有可能诱导细胞炎症相关蛋白及mRNA的表达； 2高于生理水平的CRP对内皮细胞的致炎作用主要通过诱导细胞凋亡及激活细胞增殖相关蛋白信号通路的表达； 3CRP是一种双面五聚体蛋白，结构具有特殊的二相性，能够分别结合内皮细胞和E-LDL。

After induction, a depolarizing or non-depolarizing neuromuscular blocking drug can be given intravenously to facilitate tracheal intubation.

吸入诱导：吸入一氧化氮加上强有力的挥发性麻醉剂（如氟烷、安氟醚或异氟醚），能在3－5分钟内诱导麻醉，诱导后可经静脉给予去极化或非去极化的神经肌肉阻滞剂，以利于气管插管。

Using this model three competence-deficient pneumococci, which was identified by PCR and transformation experiment, were constructed. It is found different strain has different bacterial optimal density for being induced into competence. The optimal density for strains 1, 2 and 22 was 0.1, 0.08and 0.07 respectively. The optimal condition for transformation is c plus y culture at pH8.0. In c plus y culture at pH6.8, which restrained pneumococci forming transformation naturally, pneumococci could be induced to form transformation by CSP.It is concluded that we could induce different S.pneumoniae by CSP into competence in their optimal density in laboratory. 2. It was discussed the relationship between pneumococcal transformation formation and its opportunistic pathogenesis. Comparing their virulence with that of parental strains by challenging mice intraperitoneally and adhering to ECV-304 cell respectively.

结果表明不同肺炎链球菌株的转化最适菌密度不同，1、2和22号菌株的最适转化密度分别为0.1、0.08和0.07；用于转化的CTM培养基最适为pH 8.0的C+Y培养基；在自然转化下不能诱导转化发生的pH 6.8的C+Y培养基中，使用CSP诱导，也能促使细菌发生转化，只是转化效率较低；结论：实验室条件下，不同细菌在一定菌密度时可利用CSP诱导肺炎链球菌成为感受态发生转化。2、为探讨肺炎链球菌转化对毒力表达的影响，本研究分别通过体外粘附人脐静脉血管内皮细胞ECV-304和体内小鼠毒力实验观察1、2和22号肺炎链球菌和其转化缺陷菌株1t、2t和22t毒力的差异。

RESULTS: At 7 and 14 days following osteogenic induction, a significant positive reaction to alkaline phosphatase staining was detected, with calcium nodus.

结果：成骨诱导7， 14d，经诱导的骨髓基质干细胞和脂肪间充质干细胞呈碱性磷酸酶阳性，有钙结节形成，未诱导组呈阴性。

ResultsThe Ziziphus jujube leaf extract could inhibit autonomic activities of the mice, prolong the sleep time induced by pentobarbital sodium, enhance the rate of falling asleep induced by barbital sodium and inhibit the central stimulating effects induced by amphetamine.

结果酸枣叶提取物可对小鼠自主活动呈明显抑制作用，延长戊巴比妥钠诱导的小鼠睡眠时间，增加巴比妥钠诱导的小鼠睡眠发生率和缩短巴比妥钠诱导的小鼠睡眠潜伏期。

In Gravitarmata margarotana oviposition selection period, the total contents of volatiles in healthy needle branches were higher than healthy cone branches, and the total contents of volatiles in red cone tree were lower than green cone tree.

在油松球果小卷蛾危害期，虫害果枝中挥发物的含量均高于健康果枝，油松健康果枝挥发物的含量在化学诱导后明显增加，化学诱导与植食性昆虫取食对油松产生了相似的诱导反应。

The results showed that thrombin elevated the activity of RhoA and the binding capability of RhoA with mDia1 during thrombin-induced platelet aggregation and spreading on Fg coated coverslips. Wortmannin inhibited the rising of RhoA activity and the binding level of RhoA with mDia1 induced by thrombin.

结果表明：凝血酶能够诱导聚集血小板中RhoA活性上调，且与mDia1相结合的RhoA-GTP显著增高；渥曼青霉素能够阻断由凝血酶诱导的聚集血小板中RhoA活性上调，并阻断凝血酶诱导的RhoA-GTP与mDia1的结合。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。